Effects of cutting fluids on drilling aluminium casting alloy 390

The primary objective of this research project was to compare the tool wear resulting when a semi-synthetic cutting fluid was used, to that from use of a premium soluble oil. The secondary objective was to determine the effects of cutting speed, drill diameter, and fluid concentration, on tool wear. The experiments contained two key elements: (1) cast aluminium alloy 390 was used as the workpiece material and (2) deep-hole drilling to a depth greater than two diameters was performed. Numerous drilling tests were performed with each cutting fluid using a flood application. To meet the primary objective, an experiment was designed for each cutting fluid and the wear on the lands of twist drills was measured. The secondary objective utilized a statistically-designed 2³ factorial experiment which evaluated the effects that moderate and relatively high cutting speeds, two drill diameters, and two levels of fluid concentration, had on tool wear. After analysis of the test results, it was concluded that concentration had the most significant effect on land wear when the experimentally-formulated semi-synthetic cutting fluid was used. Cutting speed was the most significant factor when drilling with the commercially-available premium soluble oil. Tool wear while using the semi-synthetic fluid was slightly less than that for the premium soluble oil.     

Anticonvulsive Effects and Pharmacokinetic Profile of Cannabidiol (CBD) in the Pentylenetetrazol (PTZ) or N-Methyl-D-Aspartate (NMDA) Models of Seizures in Infantile Rats

In spite of use of cannabidiol (CBD), a non-psychoactive cannabinoid, in pediatric patients with epilepsy, preclinical studies on its effects in immature animals are very limited. In the present study we investigated anti-seizure activity of CBD (10 and 60 mg/kg administered intraperitoneally) in two models of chemically induced seizures in infantile (12-days old) rats. Seizures were induced either with pentylenetetrazol (PTZ) or N-methyl-D-aspartate (NMDA). In parallel, brain and plasma levels of CBD and possible motor adverse effects were assessed in the righting reflex and the bar holding tests. CBD was ineffective against NMDA-induced seizures, but in a dose 60 mg/kg abolished the tonic phase of PTZ-induced generalized seizures. Plasma and brain levels of CBD were determined up to 24 h after administration. Peak CBD levels in the brain (996 ± 128 and 5689 ± 150 ng/g after the 10- and 60-mg/kg doses, respectively) were reached 1–2 h after administration and were still detectable 24 h later (120 ± 12 and 904 ± 63 ng/g, respectively). None of the doses negatively affected motor performance within 1 h after administration, but CBD in both doses blocked improvement in the bar holding test with repeated exposure to this task. Taken together, anti-seizure activity of CBD in infantile animals is dose and model dependent, and at therapeutic doses CBD does not cause motor impairment. The potential risk of CBD for motor learning seen in repeated motor tests has to be further examined.     

The Relation between Calcium Sequestering Capacity and Oxidation Degree of Dicarboxy-Starch and Dicarboxy-Inulin

The objective of this study was to establish the relation between the calcium sequestering capacity (SC) of dicarboxy-starch and that of dicarboxy-inulin, and the oxidation degree of these products. An S-type curve is obtained when plotting the SC of oxidized starch versus the degree of oxidation. By contrast the sequestering capacity of dicarboxy-inulin increases linearly with the degree of oxidation. The difference in behaviour of these materials can be attributed to the fact that upon starch oxidation the favourable oxydiacetate structure is obtained only after substantial conversion, whereas in inulin this structure is obtained immediately and is present in each oxidized fructose unit.     

Synthesis of Alkyl Fructosides

The anomeric alkylation of D-fructose is efficiently catalyzed by mild acid catalysts. Degradation of fructose is negligible under the conditions applied.     

自由之鸟与牢笼:二战后十年间荷兰建筑期刊考

本文追溯了二战后十年间《建造周刊》《论坛》《天主教建筑期刊》等重要期刊的创立(重建)与发展的历史,管窥战后荷兰专业建筑期刊业的全貌。文章讨论了展现在战后荷兰建筑期刊中的多元思想——米拉斯所代表的中间路线,J·B·巴克马、J·H·凡·登·布鲁克、A·E·凡·艾克代表的新一代先锋思潮,J·朗普雷·莫里哀领导下的战后传统主义,巴克马对"自由"的解读等。本文是一篇呈现荷兰战后建筑界思想发展驱动力与思想交锋的文章。     

Coordination‐Assisted Bioorthogonal Chemistry: Orthogonal Tetrazine Ligation with Vinylboronic Acid and a Strained Alkene

Bioorthogonal chemistry can be used for the selective modification of biomolecules without interfering with any other functionality that might be present. Recent developments in the field include orthogonal bioorthogonal reactions to modify multiple biomolecules simultaneously. During our research, we observed that the reaction rates for the bioorthogonal inverse‐electron‐demand Diels–Alder (iEDDA) reactions between nonstrained vinylboronic acids (VBAs) and dipyridyl‐s‐tetrazines were exceptionally higher than those between VBAs and tetrazines bearing a methyl or phenyl substituent. As VBAs are mild Lewis acids, we hypothesised that coordination of the pyridyl nitrogen atom to the boronic acid promoted tetrazine ligation. Herein, we explore the molecular basis and scope of VBA–tetrazine ligation in more detail and benefit from its unique reactivity in the simultaneous orthogonal tetrazine labelling of two proteins modified with VBA and norbornene, a widely used strained alkene. We further show that the two orthogonal iEDDA reactions can be performed in living cells by labelling the proteasome by using a nonselective probe equipped with a VBA and a subunit‐selective VBA bearing a norbornene moiety.     

Continuous operation of membrane bioreactor treating toluene vapors by Burkholderia vietnamiensis G4

A laboratory-scale biofilm membrane bioreactor inoculated with Burkholderia vietnamiensis G4 was examined to treat toluene vapors in a waste gas stream. The gas feed side and nutrient solution were separated by a composite membrane consisting of a porous polyacrylonitrile (PAN) support layer coated with a very thin (0.3 μm) dense polydimethylsiloxane (PDMS) top layer. After inoculation, a biofilm developed on the dense layer. The biofilm membrane bioreactor was operated continuously at different residence times (28–2 s) and loading rates (1.2–26.7 kg m⁻³ d⁻¹), with inlet toluene concentrations ranging from 0.21 to 4.1 g m⁻³. The overall performance of the membrane bioreactor was evaluated over a period of 165 days. Removal efficiencies ranging from 78% to 99% and elimination capacities from 4.2 to 14.4 kg m⁻³ d⁻¹ were observed after start-up period depending on the mode of operation. A maximum elimination capacity of 14.4 kg m⁻³ d⁻¹ was observed at a loading rate of 17.4 kg m⁻³ d⁻¹. Overall, the results illustrate that biofilm membrane reactors can potentially be more effective than conventional biofilters and biotrickling filters for the treatment of air pollutants such as toluene.     

Antioxidant and antiproliferative activities of mango (Mangifera indica L.) flesh and peel

The antioxidant and antiproliferative properties of flesh and peel of mango (Mangifera indica L.) were investigated. The cytoprotective effect of mango flesh and peel extracts on oxidative damage induced by H₂O₂ in a human hepatoma cell line, HepG2, were determined, and the underlying mechanism was examined by a single-cell electrophoresis assay (comet assay). Treatment of HepG2 cell with mango peel extract prior to oxidative stress was found to inhibit DNA damage. The free radical scavenging activities of mango flesh and peel extracts were evaluated by electron spin resonance (ESR). The mango peel extract exhibited stronger free radical scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radicals than mango flesh extract, regardless of ripeness. Similarly, peel extract exhibited significant antiproliferative effect against all tested cancer cell lines, compared to that of flesh extract, in a dose-dependent manner. The result also showed that the antiproliferative activity of mango flesh and peel extracts correlated with their phenolic and flavonoid contents. Thus, mango peel, a major by-product obtained during the processing of mango product, exhibited good antioxidant activity and may serve as a potential source of phenolics with anticancer activity.