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991.
992.
Alkali-tolerant Aspergillus fischeri Fxn1 produced two extracellular xylanases. The major xylanase (M(r) 31,000) was purified to electrophoretic homogeneity by ammonium sulfate precipitation, anion exchange chromatography and preparatory PAGE. Xylose was the major hydrolysis product from oat spelt and birch wood xylans. It was completely free of cellulolytic activities. The optimum pH and temperature were 6.0 and 60 degrees C, respectively. pH stability ranged from 5 to 9.5 and the t1/2 at 50 degrees C was 490 min. It had a Km of 4.88 mg ml-1 and a Vmax of 588 mumol min-1 mg-1. The activity was inhibited (95%) by AlCl3 (10 mM). This enzyme appears to be novel and will be useful for studies on the mechanism of hydrolysis of xylan by xylanolytic enzymes. 相似文献
993.
The role of activation functions in feedforward artificial neural networks has not been investigated to the desired extent. The commonly used sigmoidal functions appear as discrete points in the sigmoidal functional space. This makes comparison difficult. Moreover, these functions can be interpreted as the (suitably scaled) integral of some probability density function (generally taken to be symmetric/bell shaped). Two parameterization methods are proposed that allow us to construct classes of sigmoidal functions based on any given sigmoidal function. The suitability of the members of the proposed class is investigated. It is demonstrated that all members of the proposed class(es) satisfy the requirements to act as an activation function in feedforward artificial neural networks. 相似文献
994.
Sankata Bhanjan Prusty Sridhar Seshagiri Umesh Chandra Pati Kamala Kanta Mahapatra 《IEEE/CAA Journal of Automatica Sinica》2020,7(1):118-125
For the problem of set point regulation of the liquid level in coupled tank systems, we present a continuous sliding mode control(SMC) with a "conditional integrator", which only provides integral action inside the boundary layer. For a special choice of the controller parameters, our design can be viewed as a PID controller with anti-windup and achieves robust regulation.The proposed controller recovers the transient response performance without control chattering. Both full-state feedback as well as output-feedback designs are presented in this work. Our output-feedback design uses a high-gain observer(HGO) which recovers the performance of a state-feedback design where plant parameters are assumed to be known. We consider both interacting as well as non-interacting tanks and analytical results for stability and transient performance are presented in both the cases. The proposed controller continuous SMC with conditional integrators(CSMCCI) provides superior results in terms of the performance measures as well as performance indices than ideal SMC, continuous SMC(CSMC) and continuous SMC with conventional integrator(CSMCI). Experimental results demonstrate good tracking performance in spite of unmodeled dynamics and disturbances. 相似文献
995.
Venugopalan Visha Kumari Purabi Banerjee Vivek Chandra Verma Suvana Sukumaran Malamal Alickal Sarath Chandran Kodigal A. Gopinath Govindarajan Venkatesh Sushil Kumar Yadav Vinod Kumar Singh Neeraj Kumar Awasthi 《International journal of molecular sciences》2022,23(15)
By the year 2050, the world’s population is predicted to have grown to around 9–10 billion people. The food demand in many countries continues to increase with population growth. Various abiotic stresses such as temperature, soil salinity and moisture all have an impact on plant growth and development at all levels of plant growth, including the overall plant, tissue cell, and even sub-cellular level. These abiotic stresses directly harm plants by causing protein denaturation and aggregation as well as increased fluidity of membrane lipids. In addition to direct effects, indirect damage also includes protein synthesis inhibition, protein breakdown, and membranous loss in chloroplasts and mitochondria. Abiotic stress during the reproductive stage results in flower drop, pollen sterility, pollen tube deformation, ovule abortion, and reduced yield. Plant nutrition is one of the most effective ways of reducing abiotic stress in agricultural crops. In this paper, we have discussed the effectiveness of different nutrients for alleviating abiotic stress. The roles of primary nutrients (nitrogen, phosphorous and potassium), secondary nutrients (calcium, magnesium and sulphur), micronutrients (zinc, boron, iron and copper), and beneficial nutrients (cobalt, selenium and silicon) in alleviating abiotic stress in crop plants are discussed. 相似文献
996.
Qin Zhang Sangsu Bang Sharat Chandra Ru-Rong Ji 《International journal of molecular sciences》2022,23(22)
Inflammation is known to cause pain, and pain is of one of the cardinal signs of inflammation. Mounting evidence suggests that acute inflammation also resolves pain through specialized pro-resolving mediators (SPMs) and macrophage signaling. GPR37 is expressed by neurons and oligodendrocytes in the brain and has been implicated in multiple disorders, such as demyelination, Parkinson’s disease, stroke, and cancer. Recent studies have demonstrated that GPR37 is expressed by macrophages and confers protection against infection by bacteria and parasites. Furthermore, GPR37 promotes the resolution of inflammatory pain and infection-induced pain, as the duration of pain after tissue injury and infection is prolonged in mice lacking Gpr37. Mechanistically, activation of GPR37 enhances macrophage phagocytosis, and Gpr37-deficient macrophages exhibit dysregulations of pro-inflammatory and anti-inflammatory cytokines, switching from M2- to M1-like phenotypes. We also discuss novel ligands of GPR37, including neuroprotectin D1 (NPD1), a SPM derived from docosahexaenoic acid (DHA), and bone-derived hormone osteocalcin (OCN), which can suppress oligodendrocyte differentiation and myelination. NPD1 stimulates macrophage phagocytosis via GPR37 and exhibits potent analgesic actions in various animal models of inflammatory and neuropathic pain. Targeting GPR37 may lead to novel therapeutics for treating inflammation, infection, pain, and neurological diseases. 相似文献
997.
Abhijit Chandra Grant Anderson Shreyes Melkote Wei Gao Han Haitjema Konrad Wegener 《CIRP Annals》2014,63(2):797-819
Solar cells gained significant interest recently due to the rapid increase in fossil fuel costs and renewed attention to sustainability. The effectiveness of a solar cell in energy harvesting hinges on three key characteristics: efficiency of energy conversion, reliability and life expectancy, and cost. All three attributes are critically influenced by surface and interface properties inherent in the design and manufacture of these devices. This paper starts with an exposition of solar cell manufacturing, followed by discussion of efficiency enhancement, reliability issues, and cost and energy footprint reduction. The role of surface and interface modifications in realizing such improvements is assessed. 相似文献
998.
Arbaz Khan Pravir Dutt Chandra Shekhar Upadhyay 《Journal of scientific computing》2017,73(2-3):876-905
In this paper, a least-squares spectral element method for parabolic initial value problem for two space dimension on parallel computers is presented. The theory is also valid for three dimension. This method gives exponential accuracy in both space and time. The method is based on minimization of residuals in terms of the partial differential equation and initial condition, in different Sobolev norms, and a term which measures the jump in the function and its derivatives across inter-element boundaries in appropriate fractional Sobolev norms. Rigorous error estimates for this method are given. Some specific numerical examples are solved to show the efficiency of this method. 相似文献
999.
Molecular identification and characterization of Fusarium spp. associated with sorghum seeds
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1000.
The development of tissue engineering in the field of orthopedic surgery is booming. Two fields of research in particular have emerged: approaches for tailoring the surface properties of implantable materials with osteoinductive factors as well as evaluation of the response of osteogenic cells to these fabricated implanted materials (hybrid material). In the present study, we chemically grafted insulin onto the surface of hydroxyapatite nanorods (nHA). The insulin-grafted nHAs (nHA-I) were dispersed into poly(lactide-co-glycolide) (PLGA) polymer solution, which was electrospun to prepare PLGA/nHA-I composite nanofiber scaffolds. The morphology of the electrospun nanofiber scaffolds was assessed by field emission scanning electron microscopy (FESEM). After extensive characterization of the PLGA/nHA-I and PLGA/nHA composite nanofiber scaffolds by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), energy-dispersive X-ray spectrometry (EDS), and transmission electron microscopy (TEM), the PLGA/nHA-I and PLGA/nHA (used as control) composite nanofiber scaffolds were subjected to cell studies. The results obtained from cell adhesion, alizarin red staining, and Von Kossa assay suggested that the PLGA/nHA-I composite nanofiber scaffold has enhanced osteoblastic cell growth, as more cells were proliferated and differentiated. The fact that insulin enhanced osteoblastic cell proliferation will open new possibilities for the development of artificial scaffolds for bone tissue regeneration. 相似文献