Construction of castor functional markers fingerprint and analysis of genetic diversity

In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources, fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers (FMs) associated with fatty acid metabolism-related genes. A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103–0.695. Shannon’s information index (I), observed heterozygosity (Ho) and expected heterozygosity (He) were 0.699, 0.188 and 0.436 respectively. The clustering results indicated that the castor germplasm could be divided into two groups with the genetic similarity coefficient of 0.59. The genetic similarity of 12 regions ranged from 0.518 to 0.917 and the genetic distance was between 0.087 and 0.658. A total of 5 pairs of core primers were screened to construct a digital fingerprint of different castor germplasm resources, which could distinguish all 52 germplasms. This study provides a scientific basis for screening high-quality castor germplasm resources and broadening the genetic basis of castor breeding at the molecular level.     

CHARACTERIZATION OF A SALT-TOLERANT ACID PROTEASE PRODUCED BY BACILLUS MEGATERIUM KLP-98 AND ITS POTENTIAL AS A FERMENTATION STARTER FOR THE MANUFACTURE OF FISH SAUCE

A salt-tolerant acid protease was purified from the culture broth of Bacillus megaterium KLP-98 with a molecular weight of 64 kDa. K _m and k _cat values of the protease were 2.1 mg/mL and 6.1 s⁻¹ against azocasein as a substrate, respectively. The optimal temperature for protease activity was 60C but optimal stability temperature was below 40C. The optimal pH for protease activity was 5.5, and it was stable from pH 4.5 to 6.0. Relative activity of the protease was 73, 33, and 5% at 10, 20, and 30% NaCl concentrations, respectively. Among all the substrates tested, the best substrate for B. megaterium KLP-98 was azocasein followed by Z-Phe-Arg-NMec. The protease activity was strongly inhibited by N-ethylmaleimide and E-64. This protease was therefore presumed to be a salt-tolerant acid cysteine protease. The purified protease was applied to accelerate the fermentation of anchovy sauce, and the results indicated its potential as a fermentation starter.

PRACTICAL APPLICATIONS

The B. megaterium KLP-98 protease accelerated the fermentation of anchovy sauce and greatly improved its quality only during 2 days of fermentation, thus will decrease its production cost. Hence, fish or soy sauce industries can benefit from this protease as a fermentation accelerant.     

图形化编程中模块间并行性的自动挖掘

在基于模块组合的图形化编程中,存在大量互不依赖的模块,这些模块具有并行执行的性质。翻译程序以拓扑排序算法遍历该有向无环图,为每个模块产生一个线程,为每条输入线产生一个信号量,以同步有依赖关系的模块的执行顺序,最终产生一个可并行执行的多线程程序,从而达到自动挖掘并行性、提高所生成程序的运行效率的目的。     

A STUDY OF THE INTERACTION OF DISLOCATIONS WITH γ′-PRECIPITATES IN NICKEL-BASE HIGH TEMPERATURE ALLOYS

提供了位错切割γ′相的实验证明,并指出研究γ′相的结构与性能对了解其强化作用的重要意义。     

Improvement to the scanning electron microscope image colourization by adaptive tuning

An improvement to the previously proposed Canny optimization technique for scanning electron microscope image colourization is reported. The additional process is adaptive tuning, where colour tuning is performed adaptively, based on comparing the original luminance values with calculated luminance values. The complete adaptive Canny optimization technique gives significantly better mechanical contrast on scanning electron microscope grey-scale images than do existing methods.     

网格安全模型的设计

用一个实例来阐述了网格环境下的安全问题,并对安全问题进行了分析,在此基础上给出了一个网格的安全模型,较好的解决了单点登录、资源代理和资源分配.     

长渣和短渣的数值判据

在研究硅酸盐熔体和氟化钙基熔渣粘度随温度变化规律的基础上，引入最小曲率半径Ｒ（ｍｉｎ）的概念作为“长渣”和“短渣”的数值判据。Ｒ（ｍｉｎ）值隐含了表征熔体粘度表现活化能Ｅη，熔化半球点温度Ｔ０和在特征温度ＴＲ的熔体粘度值ηＲ。以ＣａＦ、ＳｉＯ２作为典型“短渣”、“长渣”，对所研究的渣系进行分类。不同熔剂或熔渣的Ｒ_（ｍｉｎ）计算值对电渣重熔、保护浇铸以及焊接的工艺过程有一定指导意义。     

具有不确定参数多目标决策的一类鲁棒有效解

针对具有区间型不确定参数多目标决策问题,探讨了决策人在决策的最优性与决策后果 的不确定性之间的权衡,从而制定决策的方法,提出了鲁棒有效解及ε-鲁棒有效解的概念,并研究 了其最优性条件.