Design of new inhibitors for cdc2 kinase based on a multiple pseudosubstrate structure

New inhibitors have been designed for cdc2 kinase based on a multiple pseudosubstrate structure. The new inhibitors have three different structural components: 3,4-bis(indol-3-yl)maleimide, Ac-Cys-(Ser)-Pro-Lys-Lys-NHMe, and ethyloxy group between the two components. Inhibitory activities toward cdc2 and other protein kinases were investigated, and the compound (21) with Ac-Cys-Pro-Lys-Lys-NHMe connected with the triethylene glycol spacer exhibited the most potent inhibition with relatively high selectivity.     

Dynamics of Hollow and Solid Alumina Particle Formation in Spray Flames

Thermophoretic sampling (TS) of the aerosol was conducted to manifest the formation of hollow and solid alumina particles in spray flames. The collected particles were investigated by transmission electron microscopy. Hollow particles with a thin shell (e.g., 10 nm) were formed from the aluminum nitrate precursor emulsion at less than 4-cm flame height. Hollow particles maintained their shapes in the flame using air as dispersion/oxidant gas, whereas hollow-to-solid restructuring of the particles took place in the flame using oxygen. With oxygen, nanoparticles were formed in the gas phase from the aluminum butoxide/2-propanol precursor solution only, whereas gas-phase reaction was hindered, forming large particles from the aluminum nitrate/2-propanol precursor solution.     

Pipe Jacking Method for Long Curve Construction

The D301 pipe jacking method is the first to accomplish long spans, curves and high speed construction in the field of small diameter (300 mm) tunneling. The design of the system components are described: tunneling machine with directional control ability, jacking machine, power unit, and control unit which contains a microprocessor. As a result of field tests in about 100 m in length with a 200 curvature radius, the following capabilities are confirmed: the span length is up to 100 m, the curvature radius is down to 200 m, it is useable in either cohesive or sandy soils having N‐curves up to 10, and the construction speed is up to 3 m∕h.     

Non-degenerate Linear Codes Determined by their Weight Enumerators

 In [1], Homma-Kim-Yoo showed that a code C such that the support of the associated projective system is a union of two linear subspaces, is uniquely determined up to equivalence by its weight enumerator. Let W _C (z) be the weight enumerator of a code C. Assuming certain conditions on the coefficients of W _C (z) for some range, we prove that the support of the associated projective system is a union of two linear subspaces. Received: February 27, 2002; revised version: October 2, 2002 Keywords: Linear code, Projective system, Weight enumerator. MSC2000: 94B27, 94B65, 51E20 Partially supported by Grant-in-Aid for Scientific Research, Ministry of Education, #12640180     

Development of a deep-hole, laser-guide boring tool: the boring of workpieces with a thin wall and an inclined prebored hole

A laser-guided BTA tool has been developed to prevent axial hole deviations. Four kinds of experiments were conducted to examine the performance of this tool in detail and to determine its practical application using a duralumin (A2017-T4) workpiece with a prebored 108-mm diameter hole. The first experiment was performed to examine the conditions of the hole deviation in the case of normal deep-hole boring. The second was to examine whether the tool corrects for the stated hole deviation. The third was to examine whether the tool can go straight through to the target without shifting toward a thin wall (t = 0.7 mm) on one side of the workpiece. The fourth was to examine whether the tool can be guided toward the target without affecting a prebored hole, which was inclined to radial direction at 100 μm for a hole length of 100 mm. The experiments were performed with a rotating tool-stationary workpiece system. Rotational speed was 270 rpm and feed ws 0.125 mm/rev. Tool diameter was 110 mm. The results show that the tool can be guided to go straight through toward the target despite the disturbances.     

Structural analysis of oligosaccharides derivatized with 4-aminobenzoic acid 2-(diethylamino)ethyl ester by matrix-assisted laser desorption/ionization mass spectrometry

Oligosaccharides derivatized with 4-aminobenzoic acid 2-(diethylamino) ethyl ester (ABDEAE) can be analyzed by ESI (Yoshino, K.; et al. Anal. Chem. 1995, 67, 4028-4031) and MALDI (Takao, T.; et al. Rapid Commun. Mass Spectrom. 1996, 10, 637-640) mass spectrometry. In this study, oligosaccharides derived from the enzymatic cleavage of the sugar chains of glycoproteins ribonuclease B, erythropoietin, and transferrin were subjected to ABDEAE derivatization, prior to analysis on a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) for high-resolution mass measurement and a postsource decay (PSD) experiment. In the mass measurement of ABDEAE derivatives, quasi-molecular ion species have been observed in monoisotopic resolution using 2,5-dihydroxybenzoic acid as the matrix from spots that contain 50-200 fmol of sample; in the PSD analyses from the spots contained 500 fmol-1 pmol of sample, the predominant backbone ion series which covers the entire mass range for all the derivatives, the internal ion series which reflect the branched trimannosyl core structure of N-glycans, and the low m/z fingerprint ion of ABDEAE were consecutively observed, permitting structure elucidation of the oligosaccharides. Given the effectiveness of this derivatization in terms of its high sensitivity and resolution with respect to MALDI-TOF MS, current methodology is clearly applicable to the sensitive detection and accurate structural analysis of N-glycans.     

Design for 2-D error feedback networks with identical coefficient sets

In realization of recursive digital filters with fixed point arithmetic, an error caused by roundoff arises. It is known that the level of the roundoff noise of an IIR filter tends to be high when the poles are close to the unit circle. Error feedback (EF) is an effective method to reduce the roundoff noise. It is desirable to design an EF network using as few parameters as possible in order to keep computational costs low. In this paper, we propose a method for designing a 2D EF network with identical coefficient sets. That is, the EF coefficients are divided into several subsets such that all the elements within each set have the same absolute value. In order to optimize the coefficient sets, we propose an algorithm by using the genetic algorithm. In the numerical example, we demonstrate the effectiveness of the proposed method.     

Evolution of nursing at University Hospitals: depositions by nurses who worked during the seventies

This study is part of a more extensive project that proposes to recover significant aspects related to nursing care evolution from 1950s until 1990s. This study is developed through the technique of oral declaration by active and retired registered nurses, in the context of an university-hospital from S?o Paulo State. The present study emphasizes the outcomes referring to the 1970s. As result becomes evident the undertaken effort by nurses in the struggle for profession's recognition and prestige; intense and deep transformations related to nurse's new roles as leadership of nursing staff and member of medical team.