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11.
12.
Abe Schwartz Lili Wang Edward Early Adolfas Gaigalas Yu-zhong Zhang Gerald E. Marti Robert F. Vogt 《Journal of research of the National Institute of Standards and Technology》2002,107(1):83-91
The quantitation of fluorescence radiance may at first suggest the need to obtain the number of fluorophore that are responsible for the measured fluorescence radiance. This goal is beset by many difficulties since the fluorescence radiance depends on three parameters 1) the probability of absorbing a photon (molar extinction), 2) the number of fluorophores, and 3) the probability of radiative decay of the excited state (quantum yield). If we use the same fluorophore in the reference solution and the analyte then, to a good approximation, the molar extinction drops out from the comparison of fluorescence radiance and we are left with the comparison of fluorescence yield which is defined as the product of fluorophore concentration and the molecular quantum yield. The equality of fluorescence yields from two solutions leads to the notion of equivalent number of fluorophores in the two solutions that is the basis for assignment of MESF (Molecules of Equivalent Soluble Fluorophore) values. We discuss how MESF values are assigned to labeled microbeads and by extension to labeled antibodies, and how these assignments can lead to the estimate of the number of bound antibodies in flow cytometer measurements. 相似文献
13.
The total syntheses of 9(Z),12(E)- and 9(E),12(Z)-octadecadienoic acids have been carried out. A useful intermediate in both
syntheses, 8-bromo-octanoic acid, recently has become available from commercial sources. This compound has been used to expedite
the preparation of these isomers. The remaining carbon atoms were derived from propargyl alcohol along with either 1-heptyne
or acetylene and 1-bromopentane. Because the overall yield for each sequence was roughly 15% and there were no extraordinary
reaction conditions in any of the synthetic steps, the compounds could be prepared readily in multiple gram quantities. The
syntheses of the two compounds were supported by data from a variety of spectroscopic techniques. 相似文献
14.
In Part I of this paper, a theoretical basis is presented using a two-degrees-of-freedom model. In this second part of the study, the passive control and the two types of semiactive controls introduced in Part I are examined numerically for a taut cable experiencing wind-induced galloping motion. The passive and the semiactive control schemes for taut cables show a good similarity with the results obtained for the two-degrees-of-freedom model. The potential of using these control schemes in practical applications to flexible structures is demonstrated. 相似文献
15.
Anju Sawada Takehiro Kubo Yosikazu Abe Kazuyuki Matsumoto 《Journal of Low Temperature Physics》1995,101(3-4):707-712
The isochoric pressure of bcc Solid3He in the vicinity of the triple point has been measured by changing the temperature stepwisely and waiting for thermal equilibrium. We observed anomalous pressure kinks of the temperature dependence in the paramagnetic region near the triple point. This anomaly appeared at 0.37 to 0.40 tesla, and disappeared below 0.36 tesla. Moreover, critical phenomena at the transition point from the paramagnetic phase (PP) to the high field phase was observed and the phase line curves toward the PP side. 相似文献
16.
M. Hara Y. Hatano T. Abe K. Watanabe T. Naitoh S. Ikeno Y. Honda 《Journal of Nuclear Materials》2003,320(3):265-271
To improve the durability of hydrogen storage materials against surface poisoning by impurity gases, effectiveness of Pd-coating layer prepared by using a Barrel-Sputtering System was examined for ZrNi powder. The effectiveness of Pd-coating was evaluated by activation temperature, at which Pd/ZrNi poisoned by air could be activated to absorb hydrogen. Characterization of Pd-coated ZrNi (denoted as Pd/ZrNi) by scanning electron microscopy, electron probe microanalysis and X-ray diffraction showed that a uniform Pd-coating layer was formed with the barrel-sputtering system. It was found that the poisoned Pd/ZrNi sample could be activated even at 423 K to absorb hydrogen at room temperature. This exhibits remarkable contrast to bare ZrNi, which could be only activated appreciably above 1073 K. It is concluded that the Pd-coating by barrel sputtering is quite effective to avoid the effect of surface poisoning of powdery hydrogen storage materials. However, the activation at excessively high temperature resulted in the loss of high activity to absorb hydrogen. It was concluded that this phenomenon was associated with reactions between Pd and ZrNi to form PdZr and other byproducts. 相似文献
17.
18.
Flexural test specimens were injection-molded from six homoisotactic polypropylenes with MFI = 0.49-25.1 dg/min under cylinder temperatures of 200-320°C. Distributions in the flow direction of higher-order structures such as crystallinity Xc, thickness of skin layer, a*-axis-oriented component fraction [A*], and crystalline orientation functions and distributions in the thickness direction of higher-order structures such as Xc, β-crystal contents, [A*], and crystalline orientation functions were studied. These higher-order structures are inhomogeneous in the flow and thickness directions, which strongly influences the product properties such as mechanical and thermal properties. Molecular orientation process in injection molding was theoretically analyzed from a viewpoint of growth of recoverable shear strain at the gate and its relaxation in the cavity, which could considerably well explain the variations in the flow and thickness directions of the quantities such as thickness of the skin layer and crystalline orientation functions which express the degree of molecular orientation. 相似文献
19.
Yamaguchi Y. Mitsumoto M. Sengoku M. Abe T. 《Geoscience and Remote Sensing, IEEE Transactions on》1992,30(1):186-189
An FM-CW radar system was applied to detect a human body buried in a very wet snowpack. This radar uses the L -band microwave frequency with a maximum output power of 100 mW, and utilizes digital signal processing techniques. Field experiments were carried out to detect and map a human body embedded at a depth of 125 cm in a natural snowpack. The radar is shown to have a potential ability to detect avalanche victims, indicating that it may become a tool for snow rescuer operations 相似文献
20.
Kazutaka Chibana Chaedong Kang Masashi Okada Koji Matsumoto Tetsuo Kawagoe 《International Journal of Refrigeration》2002,25(2):83
Water-silicone oil emulsion with an additive, (C2H5O)3SiC3H6NH2, was examined as a heat storage material. A spiral tube used as a heat exchanger was immersed in a low temperature bath and the emulsion was circulated in the tube to make ice continuously. Ice was separated from the ice–liquid suspension in an outlet tank. The amount of formed ice, the temperatures of the inlet and the outlet of the heat exchanger, and the temperatures in the tube wall were measured and the overall heat transfer coefficient and the heat flux through the tube were calculated. Experiments were carried out, varying the flow rate, the temperature of cooling brine, and the thickness of tube wall. The condition under which slurry ice was formed continuously without adhesion of ice to the cooling wall was clarified. Though decrease in the thermal resistance of the tube increased the rate of ice formation or raised the brine temperature, it narrowed the range of the flow rate and of the brine temperature in which slurry ice was formed continuously. 相似文献