In this paper the practical density of long‐term DNA storage is increased. Specifically, the DNA weight loading of silica sphere DNA storage is increased to 3.4 wt%, a ten‐fold increase compared to the previous state‐of‐the‐art. By applying a Layer‐by‐Layer (LbL) design with alternating layers of DNA and a polycationic molecule, namely polyethyleneimine (PEI), another dimension to DNA surface binding onto magnetic nanoparticles is added. A protective silica layer is grown on top of the multilayered nanoparticles to shield the DNA from external sources of damage. Accelerated aging experiments of the nanoparticles and the subsequent quantification of DNA stability via qPCR show a significantly lower degradation rate compared to unprotected DNA. The novel material is compared to previous DNA storage technologies, outperforming those in DNA storage density as well as stability. Finally, the storage of an 83 kB digital file in DNA through a successful readout of a 4991 oligonucleotide pool is demonstrated from particle encapsulation, through accelerated aging, to sequencing. 相似文献
High‐quality epitaxy consisting of Al1?xGaxN/Al1?yGayN multiple quantum wells (MQWs) with sharp interfaces and emitting at ≈280 nm is successfully grown on sapphire with a misorientation angle as large as 4°. Wavy MQWs are observed due to step bunching formed at the step edges. A thicker QW width accompanied by a greater accumulation of gallium near the macrostep edge than that on the flat‐terrace is observed on 4° misoriented sapphire, leading to the generation of potential minima with respect to their neighboring QWs. Consequently, a significantly enhanced photoluminescence intensity (at least ten times higher), improved internal quantum efficiency (six times higher at low excitation laser power), and a much longer carrier lifetime are achieved. Importantly, the wafer‐level output‐power of the ultraviolet light emitting diodes on 4° misoriented substrate is nearly increased by 2–3 times. This gain is attributed to the introduction of compositional inhomogeneities in AlGaN alloys induced by gallium accumulation at the step‐bunched region thus forming a lateral potential well for carrier localization. The experimental results are further confirmed by a numerical modeling in which a 3D carrier confinement mechanism is proposed. Herein, the compositional modulation in active region arising from the substrate misorientation provides a promising approach in the pursuit of high‐efficient ultraviolet emitters. 相似文献
The lack of cost effective, industrial‐scale production methods hinders the widespread applications of graphene materials. In spite of its applicability in the mass production of graphene flakes, arc discharge has not received considerable attention because of its inability to control the synthesis and heteroatom doping. In this study, a facile approach is proposed for improving doping efficiency in N‐doped graphene synthesis through arc discharge by utilizing anodic carbon fillers. Compared to the N‐doped graphene (1–1.5% N) synthesized via the arc process according to previous literature, the resulting graphene flakes show a remarkably increased doping level (≈3.5% N) with noticeable graphitic N enrichment, which is rarely achieved by the conventional process, while simultaneously retaining high turbostratic crystallinity. The electrolyte ion storage of synthesized materials is examined in which synthesized N‐doped graphene material exhibits a remarkable area normalized capacitance of 63 µF cm?2. The surprisingly high areal capacitance, which is superior to that of most carbon materials, is attributed to the synergistic effect of extrinsic pseudocapacitance, high crystallinity, and abundance of exposed graphene edges. These results highlight the great potentials of N‐doped graphene flakes produced by arc discharge in graphene‐based supercapacitors, along with well‐studied active exfoliated graphene and reduced graphene oxide. 相似文献
This paper presents a design of a comb finger shape and calculation of a trapezoidal-shaped electrostatic comb-drive actuator (TECA) in order to aim a higher electrostatic force density and larger displacement in comparison with the typical rectangular-shaped electrostatic comb-drive actuator (RECA). Relation between a beam’s stiffness and a driving voltage has been examined to predict a pull-in effect occurring in TECA. Micro fabrication and characterization of TECA and RECA systems are performed by using a standard SOI-MEMS technology. Theoretical and experimental results confirm the strong points of TECA’s structure (similar to the dimensions of RECA) such as a larger number of movable comb finger arrayed at the same length and larger displacement. At driving voltages of 47.9 and 50 (V), the calculation and measurement displacement of TECA are approximately 2.2 and 1.78 times larger than that of RECA, respectively.
Wireless Networks - Congestion control is necessary for enhancing the quality of service in wireless sensor networks (WSNs). With advances in sensing technology, a substantial amount of data... 相似文献
Mobile Networks and Applications - This paper proposes a new approach of digital predistortion (DPD) technique based on the adaptive indirect learning architecture (ILA) by using a recursive... 相似文献
The demand to discover every single cellular component has been continuously increasing along with the development of biological techniques. The bottom‐up approach to construct a cell‐mimicking system from well‐defined and tunable compositions is accelerating, with the ultimate goal of comprehending a biological cell. From among the available techniques, the artificial cell has been increasingly recognized as one of the most powerful tools for building a cell‐like system from scratch. This review summarizes the development of artificial cells, from a pure giant unilamellar vesicle (GUV) to a controllable, self‐fueled proteoliposome, both of which are highly compartmentalized. The basic components of an artificial cell, as well as the optimal conditions required for successful, reproducible GUV formation and protein reconstitution, are discussed. Most importantly, progress in studying the metabolic reactions in and the motility of a reconstituted artificial cell are the main focus of the review. The ability to perform a complicated reaction cascade in a controllable manner is highlighted as a promising perspective to obtaining an autonomous and movable GUV. 相似文献
This article presents a method to design broadband circularly polarized (CP) Fabry‐Perot Resonator (FPR) antenna. The proposed antenna is based on multi‐layer thin dielectric slabs arranged in close proximity as a partially reflecting surface (PRS) and an Archimedean spiral as a CP radiating source. Experimental results show a broadband operation from 6 to 13 GHz, in which the reflection coefficient is less than ?10 dB and the axial ratio (AR) is lower than 3 dB. In addition, good radiation patterns and high broadside gain of better than 10.8 dBic are achieved over the operating bandwidth. The proposed antenna can be used for C‐ and X‐band satellite communications. 相似文献
In this paper, an adaptive chattering free neural network‐based sliding mode control (ACFN‐SMC) method is proposed for tracking trajectories of redundant parallel manipulators. ACFN‐SMC combines adaptive chattering free radial basis function neural networks (RBFN), sliding mode control with online updating the robust term parameters, and a nonlinear compensation item for reducing tracking errors. The stability of the closed‐loop system with modeling uncertainties, frictional uncertainties, and external disturbances is ensured by using the Lyapunov method. The proposed controller has a simple structure and little computation time while securing dynamic performance with expected quality in tracking trajectories of redundant parallel manipulators. In addition, the ACFN‐SMC strategy does not need to know the upper bound of any uncertainties. From the simulation results, it is evident that the proposed control strategy not only has significantly higher robustness capability for uncertainties but also can achieve better chattering elimination when compared with those using existing intelligent control schemes. 相似文献
Research on assistive technology, rehabilitation, and prosthetics requires the understanding of human machine interaction, in which human muscular properties play a pivotal role. This paper studies a nonlinear agonistic‐antagonistic muscle system based on the Hill muscle model. To investigate the characteristics of the muscle model, the problem of estimating the state variables and activation signals of the dual muscle system is considered. In this work, parameter uncertainty and unknown inputs are taken into account for the estimation problem. Three observers are presented: a high gain observer, a sliding mode observer, and an adaptive sliding mode observer. Theoretical analysis shows the convergence of the three observers. Numerical simulations reveal that the three observers are comparable and provide reliable estimates. 相似文献