Anlagen‐ und Prozesstechnik für die Mikrobearbeitung mit Vakuum‐UV Excimer‐Laser

With the F₂‐Excimer laser a tool has now become available for the precise structuring of challenging materials like fused silica or PTFE (Teflon®). However, the short wavelength of 157 nm with a characteristic photon energy of 7.9 eV has special requirements regarding beam guiding and the optical components like mirrors lenses or objectives. The process and system technology introduced here is capable to produce micro‐structures with a lateral resolution in the μm‐range with a depth resolution in the sub‐μm range.     

Ruthenium‐Catalyzed Diastereoselective syn‐Cyclopropanation of Trisubstituted Alkenes with Diazoacetates

Supporting Information for this article is available on the WWW under http://www.wiley‐vch.de/home/asc Cyclopropanation of trisubstituted alkenes with diazoacetates can be achieved with remarkabe syn‐selectivity when the polymeric ruthenium(I) complex [Ru₂(CO)₄(μ‐OAc)₂]_n is used as catalyst.     

MRPC: A high performance RPC system for MPMD parallel computing

MRPC is an RPC system that is designed and optimized for MPMD parallel computing. Existing systems based on standard RPC incur an unnecessarily high cost when used on high‐performance multi‐computers, limiting the appeal of RPC‐based languages in the parallel computing community. MRPC combines the efficient control and data transfer provided by Active Messages (AM) with a minimal multithreaded runtime system that extends AM with the features required to support MPMD. This approach introduces only the necessary RPC overheads for an MPMD environment. MRPC has been integrated into Compositional C++ (CC++), a parallel extension of C++ that offers an MPMD programming model. Basic performance in MRPC is within a factor of two from those of Split‐C, a highly tuned SPMD language, and other messaging layers. CC++ applications perform within a factor of two to six from comparable Split‐C versions, which represent an order of magnitude improvement over previous CC++ implementations. Copyright © 1999 John Wiley & Sons, Ltd.     

De Novo Missense Mutations in TNNC1 and TNNI3 Causing Severe Infantile Cardiomyopathy Affect Myofilament Structure and Function and Are Modulated by Troponin Targeting Agents

Rare pediatric non-compaction and restrictive cardiomyopathy are usually associated with a rapid and severe disease progression. While the non-compaction phenotype is characterized by structural defects and is correlated with systolic dysfunction, the restrictive phenotype exhibits diastolic dysfunction. The molecular mechanisms are poorly understood. Target genes encode among others, the cardiac troponin subunits forming the main regulatory protein complex of the thin filament for muscle contraction. Here, we compare the molecular effects of two infantile de novo point mutations in TNNC1 (p.cTnC-G34S) and TNNI3 (p.cTnI-D127Y) leading to severe non-compaction and restrictive phenotypes, respectively. We used skinned cardiomyocytes, skinned fibers, and reconstituted thin filaments to measure the impact of the mutations on contractile function. We investigated the interaction of these troponin variants with actin and their inter-subunit interactions, as well as the structural integrity of reconstituted thin filaments. Both mutations exhibited similar functional and structural impairments, though the patients developed different phenotypes. Furthermore, the protein quality control system was affected, as shown for TnC-G34S using patient’s myocardial tissue samples. The two troponin targeting agents levosimendan and green tea extract (-)-epigallocatechin-3-gallate (EGCg) stabilized the structural integrity of reconstituted thin filaments and ameliorated contractile function in vitro in some, but not all, aspects to a similar degree for both mutations.     

Optimization of a parallel permutation testing function for the SPRINT R package

The statistical language R and its Bioconductor package are favoured by many biostatisticians for processing microarray data. The amount of data produced by some analyses has reached the limits of many common bioinformatics computing infrastructures. High Performance Computing systems offer a solution to this issue. The Simple Parallel R Interface (SPRINT) is a package that provides biostatisticians with easy access to High Performance Computing systems and allows the addition of parallelized functions to R. Previous work has established that the SPRINT implementation of an R permutation testing function has close to optimal scaling on up to 512 processors on a supercomputer. Access to supercomputers, however, is not always possible, and so the work presented here compares the performance of the SPRINT implementation on a supercomputer with benchmarks on a range of platforms including cloud resources and a common desktop machine with multiprocessing capabilities. Copyright © 2011 John Wiley & Sons, Ltd.     

Influence of α-dicarbonyl compounds to the molecular weight distribution of melanoidins in sucrose solutions: part 1

The correlations between formation of melanoidins and the content of α-dicarbonyl compounds and colour formation were studied in Maillard reaction model sucrose solutions at high treating temperatures (130 °C) corresponding to the industrial sucrose processing. Molecular weight distribution was determined by gel permeation chromatography (GPC) and α-dicarbonyls as high reactive intermediates of the Maillard reaction were detected by RP-HPLC and GC/MS. Referring to formation of melanoidins, new knowledge could be gained. The investigations clearly point out, that individual α-dicarbonyls are involved in formation of specific molecular weight fractions. 3-Deoxyhexosulose (3-DH) and methylglyoxal are the important α-dicarbonyl compounds in thermal-treated sucrose solutions. A low molecular weight fraction with a molecular weight range of <5,800 g/mol preferably occurs when 3-DH is formed in high concentrations. The high increase in intensity of a low molecular weight fraction is combined with a strong colour formation. In contrast to 3-DH, methylglyoxal promotes the formation of high molecular weight fractions between 6,600 g/mol and approximately 17,000 g/mol. Furthermore, model solutions with a high intensity of high molecular weight fractions show an early colour formation during the starting period of the reaction. Indeed, this early browning is not as intensive as the colour formation caused by the low molecular weight fraction during the later stage of the browning reaction. Out of this, 3-DH can be regarded as the most important intermediate for an intensive colour formation in sucrose solutions.