Subcutaneous suturing of the ruptured Achilles tendon under local anaesthesia

Debate concerning the appropriate treatment of ruptures of the Achilles tendon still continues. Conservative treatment can be associated with a high incidence of re-rupture and with relative weakness and lengthening of the triceps surae but with low costs. Surgical treatment contributes to a much lower incidence of re-rupture but can be associated with significant complications as well as with time loss and high costs of hospitalization. Subcutaneous reconstruction combines the advantages of surgical and non-surgical management. Operating with local anaesthesia reduces hospitalization time and operative costs; there are also almost no contraindications for the operation. The functional results in 36 patients operated on in this way are encouraging.     

Molecular ecology and polyphasic characterization of the microbiota associated with semi-dry processed coffee (Coffea arabica L.)

This work was aimed at isolating and identifying the microbiota present during the semi-dry method of coffee processing using polyphasic methods and to evaluate microbial diversity with PCR-DGGE. Samples of Coffea arabica L. were collected during different processing stages in southern Minas Gerais, Brazil. The bacterial and fungal isolates were phenotypically characterised and grouped according to the ARDRA technique, in which the 16–23S and ITS1-5.8S regions of the rDNA were sequenced for species identification. The bacterial counts varied from 3.7 to 7 log CFU g⁻¹. The yeast counts ranged from 3.4 to 6.9 log CFU g⁻¹, and the filamentous fungal population varied from 2 to 3.7 log CFU g⁻¹. Bacillus subtilis, Escherichia coli, Enterobacter agglomerans, Bacillus cereus and Klebsiella pneumoniae were the predominant bacteria detected during the processing of the coffee, and Pichia anomala, Torulaspora delbrueckii and Rhodotorula mucilaginosa were the dominant yeasts. All of the yeast and bacterial species detected by PCR-DGGE were isolated using culture-dependent methods, with the exception of one uncultivable bacterial species. Aspergillus was the most common genus among the filamentous fungal isolates. The use of polyphasic methods allowed a better characterization of the microbiota that is naturally present in semi-dry processed coffee.     

Impact of ultrafiltration and nanofiltration of an industrial fish protein hydrolysate on its bioactive properties

BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing the study of its biological activity. Successive fractionation on UF and NF membranes allowed concentration of peptides of selected sizes, without, however, carrying out sharp separations, some MW classes being found in several fractions. Peptides containing Pro, Hyp, Asp and Glu were concentrated in the UF and NF retentates compared to the unfractionated hydrolysate and UF permeate, respectively. Gastrin/cholecystokinin‐like peptides were present in the starting FPH, UF and NF fractions, but fractionation did not increase their concentration. In contrast, quantification of calcitonin gene‐related peptide (CGRP)‐like peptides demonstrated an increase in CGRP‐like activities in the UF permeate, relative to the starting FPH. The starting hydrolysate also showed a potent antioxidant and radical scavenging activity, and a moderate angiotensin‐converting enzyme (ACE)‐1 inhibitory activity, which were not increased by UF and NF fractionation. CONCLUSION: Fractionation of an FPH using membrane separation, with a molecular weight cut‐off adapted to the peptide composition, may provide an effective means to concentrate CGRP‐like peptides and peptides enriched in selected amino acids. The peptide size distribution observed after UF and NF fractionation demonstrates that it is misleading to characterize the fractions obtained by membrane filtration according to the MW cut‐off of the membrane only, as is currently done in the literature. Copyright © 2010 Society of Chemical Industry     

Measurement and evaluation of whole‐body vibration exposure in drivers of cargo vehicle compositions

The present study carries out measurements and evaluations of whole‐body vibration (WBV) exposure in drivers of a cargo vehicle composition, traveling under different conditions of roads and loading, to compare and assess the health risk through the various criteria provided by vibration exposure standards. Using the acceleration data measured in the seat, the WBV exposure of the drivers is evaluated according to the criteria provided by five standards: three different versions of ISO 2631 (Part 1, 1985 and 1997; Part 5, 2004), the European Directive 2002/44/EC and the new Brazilian regulation NR‐15 published in 2014. Comparisons investigating the differences between the standards are presented. The results of the experimental data indicate that the current version of the NR‐15 is more restrictive than the other standards considered in this paper. Thus, these results can contribute to improve the criteria used in the new NR‐15.     

VisuaLeague: Player performance analysis using spatial-temporal data

In recent years, the phenomenon of eSports has been a growing trend and consequently, in addition to players, other groups of users, including coaches and analysts, took an interest in online video games and the data extracted from them. Among many types of video games, one of the most widely played is the MOBA (Multiplayer Online Battle Arena) League of Legend (LoL) game. Similary to traditional sports, players and coaches/analysts analyse all game events, such as, players’ movements, to understand how they play to define new strategies and improve their performance. Our main goal is to get a better understanding of which visualizations techniques are more adequate to handle this type of spatio-temporal information data, associated to player performance analysis in video games. To address this goal, we inquired players to identify the analytical questions they need to support for performance analysis and designed the VisuaLeague prototype for the visualization of in-game player trajectories, using animated maps, and events during a LoL match. This paper presents a user study to evaluate the adequacy of animated maps and the analytical strategies followed by players when using spatio-temporal data to analyse player performance. The results support the adequacy of using the animated maps technique to convey information to users in this context. Moreover, they also point out towards a high degree of importance given to the spatio-temporal components of the data for player performance analysis.

     

Mixed Logical Dynamical Nonlinear Model Predictive Controller for Large‐Scale Solar Fields

This paper presents a control algorithm for reducing heat losses caused by clouds in large solar fields. The formulation is based on a Mixed Logical Dynamical (MLD) representation of the solar field plus the application of a Practical Nonlinear Model Predictive Controller (PNMPC) for calculating the optimal control action. The main purpose of the controller is to deactivate fields with inlet temperature greater than outlet temperature and to manipulate the oil flow rate of the activated fields for tracking the reference of the outlet temperature. A simplified lumped parameters model is used for prediction and simulation of the solar fields.     

A multi-objective approach for multi-material topology and shape optimization

An automated multi-material approach that integrates multi-objective Topology Optimization (TO) and multi-objective shape optimization is presented. A new ant colony optimization algorithm is presented and applied to solving the TO problem, estimating a trade-off set of initial topologies or distributions of material. The solutions found usually present irregular boundaries, which are not desirable in applications. Thus, shape parameterization of the internal boundaries of the design region, and subsequent shape optimization, is performed to improve the quality of the estimated Pareto-optimal solutions. The selection of solutions for shape optimization is done by using the PROMETHEE II decision-making method. The parameterization process involves identifying the boundaries of different materials and describing these boundaries by non-uniform rational B-spline curves. The proposed approach is applied to the optimization of a C-core magnetic actuator, with two objectives: the maximization of the attractive force on the armature and the minimization of the volume of permanent magnet material.     

Personalizing self-organizing music spaces with anchors: design and evaluation

We propose and evaluate a system for content-based visualization and exploration of music collections. The system is based on a modification of Kohonen’s Self-Organizing Map algorithm and allows users to choose the locations of clusters containing acoustically similar tracks on the music space. A user study conducted to evaluate the system shows that the possibility of personalizing the music space was perceived as difficult. Conversely, the user study and objective metrics derived from users’ interactions with the interface demonstrate that the proposed system helped individuals create playlists faster and, under some circumstances, more effectively. We believe that personalized browsing interfaces are an important area of research in Multimedia Information Retrieval, and both the system and user study contribute to the growing work in this field.