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Jian-Xin Zhao Lian Yang Zhen-Nan Gu Hai-Qin Chen Feng-Wei Tian Yong-Quan Chen Hao Zhang Wei Chen 《International journal of molecular sciences》2011,12(1):1-11
The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B1 (AFB1) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond between the residues H44 and L100. The stability of scFv (H4) increased from a GdnHCl50 of 2.4 M to 4.2 M after addition of the H44-L100 disulfide bond. Size exclusion chromatography revealed that the scFv (H44-L100) mutant existed primarily as a monomer, and no aggregates were detected. An affinity assay indicated that scFv (H4) and the scFv (H44-L100) mutant had similar IC50 values and affinity to AFB1. Our results indicate that interdomain disulfide bonds could stabilize scFv without affecting affinity. 相似文献
74.
In this work, a very facile one-pot hydrothermal synthesis approach has been developed for the preparation of ultrathin magnetite nanoplates. The hydrothermal procedure was performed by aging ferrous hydroxide under anaerobic conditions, which is known as Schikorr reaction. Ethylene glycol (EG), which was introduced to the reaction as another solvent, played a critical role in the formation process of these nanoplates. Typically, hexagonal Fe3O4 nanoplates with a thickness of 10 to 15 nm and a side length of 150 to 200 nm have been synthesized with EG/H2O = 1:1 in experiments. Our data suggest that the thickness of Fe3O4 nanoplates decreases, and the shape of the nanoplate becomes more irregular when the concentration of EG increases. The as-prepared Fe3O4 nanoplates were highly crystallized single crystals and exhibited large coercivity and specific absorption rate coefficient. 相似文献
75.
核探针技术的初步应用 总被引:1,自引:0,他引:1
使用新建成的核探针开展了阿米巴细胞中微量元素锌的含量及其分布的观察;锰矿物中微米级矿粒和矿物相的分析;深海锰结核内微米级层状结构的分析;反球化元素对球墨铸铁的影响以及微量元素的偏析造成不锈钢零件局域性断裂的研究。 相似文献
76.
基于玄武岩纤维的经济性、稳定性,通过将不同种玄武岩纤维混凝土的诸项性能与基准混凝土、聚丙烯纤维混凝土作比较,研究玄武岩纤维对混凝土性能的影响,研究结果表明玄武岩纤维使混凝土的综合性能有较为明显改善。 相似文献
77.
在零场下2~300K以及不同磁场下2~10K温度范围内,分别测量了超导Sc5Ir4Si10单晶的比热。采用4-Einstein峰拟合声子谱的方法对高温部分进行处理,成功地分离了Sc5Ir4Si10的晶格比热和电子比热。10K以下的比热遵守经典的超导理论,即晶格比热与温度的三次方成正比,电子比热符合温度的幂指数关系。4K以下电子比热的磁场关系为ΔCe/T∝Hα,其中α与温度有关。随着温度的降低α的值从0.67增大到0.76,表明Sc5Ir4Si10的超导配对可能是s波和d波混合波,并由d波向s波转化。 相似文献
78.
79.
Treatment of aniline hypersaline wastewater with a combined physical–biological method was investigated in this study. This method consisted of the physical adsorption of aniline from hypersaline effluents by resins and the biodegradation of the adsorbate and the regeneration of the adsorbent in the subsequent stage. The XDA-1 resin, selected from five commercial products, could separate aniline from salt effectively. The adsorption rate increased with the increase of salinity. Biodegradation of the aniline desorbed from the exhausted resin could be realized easily without salt interference. During the repetitive six combined operations, the percentage regeneration (PR) and NaCl separation efficiency (SE) of XDA-1 were 92.3% and 98.3%, respectively. No obvious difference could be found in pore size distribution between virgin XDA-1 and samples repeated six regeneration cycles. The PR of specific surface area and total pore volume of the bio-regenerated XDA-1 resin were 94.5% and 97.2%, respectively. It was indicated that XDA-1 could be bio-regenerated successfully and the regenerated resin could be re-utilized. 相似文献
80.
Yuan Yuan Xiang Wang Guang Jia Jia-Hui Liu Tiancheng Wang Yiqun Gu Sheng-Tao Yang Sen Zhen Haifang Wang Yuanfang Liu 《Diamond and Related Materials》2010,19(4):291-299
Because of the possible health threat of nanodiamonds (NDs) to organisms, the pulmonary toxicity and translocation of NDs in different sizes in mice were investigated after intratracheal instillation administration. Biochemical assays, ultrastructural and histopathological evaluations of the lungs of the control and the ND exposed mice were carried out at 1, 7, 14 or 28 days post-exposure. Exposure to 1.0 mg/kg NDs with an average diameter of 4 nm produced a temporary lung index increase at 1 day post-exposure. NDs did not have evident adverse effects in the lungs within the studied period according to histopathological and ultrastructural investigations. Furthermore, no lipid peroxidation of the lung was observed. On the whole, intratracheally instilled NDs are of low pulmonary toxicity. In addition, the amount of NDs in alveolar decreased with time elapsed and the macrophages burdened with NDs were clearly observed in the bronchia from 1 day to 28 days post-exposure. Thus we affirm the critical role of alveolar macrophages in the main excretion pathway of NDs from the lungs, i.e. they engulf the NDs, migrate upward to the trachea by escalator/mucociliary system and finally enter the pharynx. 相似文献