Forced degradation of Fas inhibits apoptosis in adenovirus-infected cells

DNA viruses have evolved elaborate mechanisms to overcome host antiviral defences. In adenovirus-infected cells, programmed cell death (apoptosis) induced by the cytokine tumour necrosis factor (TNF) is inhibited by several adenovirus-encoded proteins. Occupation of the cell-surface receptor Fas, a member of the TNF-receptor superfamily that is expressed on most cell types, triggers apoptosis of that cell. Here we show that the adenovirus RID (for receptor internalization and degradation) protein complex, which is an inhibitor of TNF-induced apoptosis, mediates internalization of cell-surface Fas and its destruction inside lysosomes within the cell. Fas has not previously been shown to be internalized and then degraded. RID also mediates internalization of the receptor for epidermal growth factor, but it does not affect the transferrin receptor or class I antigens of the major histocompatibility complex. Removal of Fas from the surface of adenovirus-infected cells expressing RID may allow infected cells to resist Fas-mediated cell death and thus promote their survival.     

Determining subnanomolar iron concentrations in oceanic seawater using a siderophore-modified film analyzed by infrared spectroscopy

Iron is a bioactive trace element in seawater that regulates photosynthetic carbon dioxide drawdown and export from surface waters by phytoplankton in upward of 40% of the world's oceans. While autonomous sensor arrays are beginning to provide high-resolution data on temporal and spatial scales for some key oceanographic parameters, current analytical methods for iron are not amenable to autonomous platforms because of the need for user involvement and wet chemistry-based approaches. As a result, very large gaps remain in our understanding of iron distribution and chemistry in seawater. Here we present a straightforward nanostructure-based method to measure dissolved iron in natural seawater. The device comprises an iron-specific chelating biomolecule, desferrioxamine B (DFB), covalently immobilized on a mesoporous silica film. Changes in infrared spectral signatures of the immobilized DFB upon Fe(III) complexation provide an accurate and precise measure of iron on the surface of a chip exposed to seawater. The current system has a detection limit of approximately 50 pM for a 1-L sample at pH 1.7 and was used to measure dissolved iron in subarctic Pacific waters without interference from other elements in seawater. This technology provides a major step toward obtaining accurate iron measurements on autonomous research platforms.     

Implementation of a fault tolerant distributed control system

The design aspects of the implementation of a fault tolerant distributed control sytem are reviewed, including specific strategies for processor and input/output redundancy. Actual results of component and system reliability achieved through the fault tolerant design are discussed as well as the implications of these results for the user.     

The Search for Authenticity in Drawing

In this article we describe a program for teaching drawing developed at the Oregon School of Design, discuss the philosophy behind our methods, and show some extraordinary examples of first-year student work. Our approach challenges the notion of teaching technique to beginning students. Instead, we emphasize the artifice of drawing, destroy student's preconceptions, and teach them to overcome their fear. We propose that authenticity is a state of mind, at the limits of both perception and technique, that enables students to draw things they never thought possible.     

Validation of procedures for quantitative whole-body autoradiography using digital imaging

The objective of this study was to demonstrate that tissue concentrations of radioactivity derived by digital analysis of autoradiograms were comparable to values derived from direct sampling and analysis of tissues. In addition, we describe the preparation and calibration of standards for use in quantitative whole-body autoradiography. For this study, three male Long-Evans hooded rats were administered 14C radioactivity intravenously. The animals were sectioned for whole-body autoradiography, with concomitant sampling of blood and 16 selected tissues. After 3 weeks of film exposure, the optical densities of the resulting autoradiograms were analyzed with a RAS3000 digital imaging system to estimate tissue concentrations of radioactivity. These concentrations were then compared with those obtained by direct analysis of the tissue samples. The concentrations derived from digital analysis of the autoradiograms were very highly correlated with those determined from direct tissue analysis (r = 0.956). Linear regression analysis yielded a straight line with a slope of 0.97 and a goodness of fit (r2) of 0.913. This analysis suggested that there is an approximate 1:1 correlation between concentration values determined by the two methods. Marked differences between the values derived via the two techniques were observed for only three tissues. However, this subset of the data accounted for only 6% of the total data, and the differences were probably due to contamination from adjacent tissues during excision. Overall, the concentrations of radioactivity derived from digital analysis of the autoradiograms were comparable to those derived from direct analysis of tissue samples. The results indicated that the digital analysis procedure for film can serve as a valuable adjunct to conventional tissue analysis for radioactivity.     

Neutralization of IL-12 decreases resistance to Listeria in SCID and C.B-17 mice. Reversal by IFN-gamma

Interleukin-12 (IL-12) is necessary for the production of IFN-gamma by NK cells during the generation of innate immunity and by T cells for the development of the Th1 response during specific cell-mediated immunity. Here we demonstrate that the endogenous production of IL-12 is critical to the survival of both immunocompromised SCID mice and normal C.B-17 control mice during a primary infection with Listeria monocytogenes. When IL-12 is neutralized in vivo, both strains of mice die at a normally sublethal dose of Listeria. Anti-IL-12 antibody-treated mice showed a decrease in macrophage I-Ad expression and an increase Listeria burden in the spleen. Furthermore, as has been demonstrated in vitro, these effects of IL-12 in vivo were predominantly regulated through the production of IFN-gamma. Administration of IFN-gamma simultaneously with neutralizing antibodies to IL-12 restored macrophage I-Ad expression, limited the spread of the infection, and resulted in the survival of SCID mice. Thus, IL-12 is critical for resistance to infection with Listeria monocytogenes, and this resistance is mediated through stimulation by IL-12 of IFN-gamma production. Concomitant experiments confirmed that anti-TNF antibodies also resulted in uncontrolled infection and a decrease in macrophage I-Ad expression. However, administration of IFN-gamma restored the levels of I-Ad in macrophages but did not limit Listeria growth.     

How employees respond to personal offense: The effects of blame attribution, victim status, and offender status on revenge and reconciliation in the workplace.

This study investigated the relationships between blame, victim and offender status, and the pursuit of revenge or reconciliation after a personal offense. Results from a sample of 141 government agency employees showed that blame is positively related to revenge and negatively related to reconciliation. In addition, victim–offender relative status moderated the relation between blame and revenge such that victims who blamed sought revenge more often when the offender's status was lower than their own. The victims' own absolute hierarchical status also moderated this relation such that lower, not higher, status employees who blamed sought revenge more often. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effect of crystalline structure on the trapped radical spectra of irradiated cellulose

The effects of crystalline modification of cellulose and of water on the ESR spectra generated by the trapped free radicals in gamma-irradiated celluloses were investigated for cotton cellulose I, II, III, and IV, partially decrystallized cotton cellulose, ballmilled cotton cellulose, hydrocelluloses of cellulose III and IV, and ramie. On irradiation of the celluloses, free radicals were formed on the cellulose molecule, probably following dehydrogenation or chain cleavage. The free radicals located within the less ordered or amorphous regions of the cellulose reacted readily with water and were terminated. The radicals located within the more ordered regions of the celluloses could be made accessible to reaction with water by the interaction of the celluloses with solvents which caused dimensional changes in the cellulosic structure. In the highly ordered regions of the celluloses, even after long periods of time in solvents which caused large dimensional changes in the cellulosic structure, the trapped free radicals were not terminated by reaction with solvent or water. The ESR spectra of the irradiated, dried celluloses were determined at ?160°C, the single-line spectra recorded had line widths of about 18-24 gauss. On the absorption of water by the irradiated celluloses, the ESR spectra changed and were dependent on the crystalline structure of the irradiated celluloses. The effects of different arrangements of the irradiated celluloses, as shown by their trapped radical spectra, particularly after interaction with water, were discussed.     

Physical and fine structure properties of cotton fibers swollen with trimethylbenzylammonium hydroxide

Cotton fiber was treated with aqueous trimethylbenzylammonium hydroxide (Triton B) at concentrations over the range 25%–40%. After complete removal of the swelling agent, the samples were evaluated for the extent of swelling, strength and elongation, birefringence, moisture regain, density, crystallinity, x-ray diffraction patterns, and microfibrillar morphology. Electron-microscopical examination and other evaluation of fine structure properties revealed that the nature of swelling is intercrystalline up to 30% concentration of Triton B, and intracrystalline beyond that. Although the swelling as measured by propanol-2 retention after treatment with 30% Triton B is about twice as much as that of the control, the original structure remains almost unchanged except for some gain in strength and elongation and increase in moisture regain. At 32% Triton B concentration and beyond, rapid decrystallization takes place, accompanied by a fall in birefringence, density, and crystallinity index. X-Ray analysis showed significant loss of lateral order and partial conversion of cellulose I to cellulose II at 35% and 40% Triton B concentrations. The results indicate that, used at the critical concentration of 30%, Triton B can be a useful swelling agent for cotton fibers as it opens up the fine structure of cellulose considerably without impairing any important physical properties.