Kaduna

Compared with other ancient towns like Kano, Ile-Ife or Oyo, Kaduna is relatively new in the hierarchy of towns in Nigeria. It has, however, the distinction of having being established in the large northern protectorate solely for colonial administrative purposes. Kaduna was originally built on a grid pattern, with all roads intersecting and properly named. Facilities were well planned and most town planning rules obeyed. But the pressure of urbanization, the existence of many development agencies whose activities are not coordinated and political interference in planning functions are threatening to deface the beauty of the city. The government is constantly evolving strategies to maintain and enhance the beauty of the town through various land management procedures.     

Transport of free and peptide-bound glycated amino acids: synthesis, transepithelial flux at Caco-2 cell monolayers, and interaction with apical membrane transport proteins

In glycation reactions, the side chains of protein-bound nucleophilic amino acids such as lysine and arginine are post-translationally modified to a variety of derivatives also known as Maillard reaction products (MRPs). Considerable amounts of MRPs are taken up in food. Here we have studied the interactions of free and dipeptide-bound MRPs with intestinal transport systems. Free and dipeptide-bound derivatives of N(6)-(1-fructosyl)lysine (FL), N(6)-(carboxymethyl)lysine (CML), N(6)-(1-carboxyethyl)lysine (CEL), formyline, argpyrimidine, and methylglyoxal-derived hydroimidazolone 1 (MG-H1) were synthesized. The inhibition of L-[(3)H]lysine and [(14) C]glycylsarcosine uptakes was measured in Caco-2 cells which express the H(+)/peptide transporter PEPT1 and lysine transport system(s). Glycated amino acids always displayed lower affinities than their unmodified analogues towards the L-[(3)H]lysine transporter(s). In contrast, all glycated dipeptides except Ala-FL were medium- to high-affinity inhibitors of [(14)C]Gly-Sar uptake. The transepithelial flux of the derivatives across Caco-2 cell monolayers was determined. Free amino acids and intact peptides derived from CML and CEL were translocated to very small extents. Application of peptide-bound MRPs, however, led to elevation (up to 80-fold) of the net flux and intracellular accumulation of glycated amino acids, which were hydrolyzed from the dipeptides inside the cells. We conclude 1) that free MRPs are not substrates for the intestinal lysine transporter(s), and 2) that dietary MRPs are absorbed into intestinal cells in the form of dipeptides, most likely by the peptide transporter PEPT1. After hydrolysis, hydrophobic glycated amino acids such as pyrraline, formyline, maltosine, and argpyrimidine undergo basolateral efflux, most likely by simple diffusion down their concentration gradients.     

Three‐dimensional optimisation of a fuel gas channel of a proton exchange membrane fuel cell for maximum current density

Proton exchange membrane (PEM) fuel cells operated with hydrogen and air offer promising alternative to conventional fossil fuel sources for transport and stationary applications because of its high efficiency, low‐temperature operation, high power density, fast start‐up and potable power for mobile application. Power levels derivable from this class of fuel cell depend on the operating parameters. In this study, a three‐dimensional numerical optimisation of the effect of operating and design parameters of PEM fuel cell performance was developed. The model computational domain includes an anode flow channel, membrane electrode assembly and a cathode flow channel. The continuity, momentum, energy and species conservation equations describing the flow and species transport of the gas mixture in the coupled gas channels and the electrodes were numerically solved using a computational fluid dynamics code. The effects of several key parameters, including channel geometries (width and depth), flow orientation and gas diffusion layer (GDL) porosity on performance and species distribution in a typical fuel cell system have been studied. Numerical results of the effect of flow rate and GDL porosity on the flow channel optimal configurations for PEM fuel cell are reported. Simulations were carried out ranging from 0.6 to 1.6 mm for channel width, 0.5 to 3.0 mm for channel depth and 0.1 to 0.7 for the GDL porosity. Results were evaluated at 0.3 V operating cell voltage of the PEM fuel cell. The optimisation results show that the optimum dimension values for channel depth and channel width are 2.0 and 1.2 mm, respectively. In addition, the results indicate that effective design of fuel gas channel in combination with the reactant species flow rate and GDL porosity enhances the performance of the fuel cell. The numerical results computed agree well with experimental data in the literature. Consequently, the results obtained provide useful information for improving the design of fuel cells. Copyright © 2011 John Wiley & Sons, Ltd.     

Sulfated carbohydrate compounds prevent microbial adherence by sexually transmitted disease pathogens

Heparan sulfate (HS) serves as a receptor for adherence of herpes simplex viruses, Chlamydia trachomatis, Neisseria gonorrhoeae, and, indirectly, human immunodeficiency virus. Using primary human culture systems, we identified sulfated carbohydrate compounds that resemble HS and competitively inhibit infection by these pathogens. These compounds are candidates for intravaginal formulations for the prevention of sexually transmitted diseases.     

Combining algorithms for automatic detection of optic disc and macula in fundus images

This paper proposes an efficient combination of algorithms for the automated localization of the optic disc and macula in retinal fundus images. There is in fact no reason to assume that a single algorithm would be optimal. An ensemble of algorithms based on different principles can be more accurate than any of its individual members if the individual algorithms are doing better than random guessing. We aim to obtain an improved optic disc and macula detector by combining the prediction of multiple algorithms, benefiting from their strength and compensating their weaknesses. The location with maximum number of detectors’ outputs is formally the hotspot and is used to find the optic disc or macula center. An assessment of the performance of integrated system and detectors working separately is also presented. Our proposed combination of detectors achieved overall highest performance in detecting optic disc and fovea closest to the manually center chosen by the retinal specialist.     

Amino acid composition of guineafowl's egg

Samples of eggs from two breeds (White-breasted and Pearl) of guinea hens which were housed either on deep litter or in layer cages were analysed for their content of amino acids. The protein content of whole eggs ranged from 482 to 494 mg g^?1, with a mean of 487 mg g^?1. Concentrations of the various amino acids in the egg ranged from 118 mg (tryptophan) to 752 mg (glutamic acid) per g N. The mean concentration of total sulphur amino acids was 395 mg per g N. Overall the amino acid composition of guineafowl egg was not affected by either breed or housing system.