Analysis of the botulinum neurotoxin type F gene clusters in proteolytic and nonproteolytic Clostridium botulinum and Clostridium barati

The purpose of this in vitro study was to compare both apical and coronal dye penetration when Ketac-Endo and AH-26 sealers were used with laterally condensed gutta percha. Crowns were removed from 28 teeth and the root canals were biomechanically prepared. The teeth were divided into two groups of 12-teeth each and a control group of 4 teeth. Root canals in the two experimental groups were filled with laterally condensed gutta percha and either Ketac-Endo or AH-26 sealer. The Ketac-Endo group had the coronal 3 mm of gutta percha and sealer removed and the resultant cavity was filled with Ketac-Endo alone. After the sealers had set, the root surfaces were coated with nail varnish except at the apex and at the coronal end. Positive controls had no root fillings and were coated with nail varnish in the same manner while the negative controls were sealed apically and coronally with Cavit prior to sealing the entire external root surface with nail varnish. Specimens were placed in 2% methylene blue dye in a vacuum of 660 mm of mercury for five minutes and then left immersed for a further two days. The roots were vertically sectioned to determine the following mean levels of dye penetration: Ketac-Endo, 1.08 mm apically and 6.29 mm coronally; AH-26, 0.75 mm apically and 6.67 mm coronally. Positive controls had total leakage and negative controls had no leakage. This study demonstrated that the apical and coronal seals obtained with Ketac-Endo and AH-26 were not significantly different although the apical seal obtained with each material was significantly better than the corresponding coronal seal.     

Theoretical thermoelectric figure of merit of highly disordered lead telluride type materials

A quantitative estimate is made of the reduction in lattice thermal conductivity (λ_L) of lead telluride type material as a function of doping level, grain size, alloying and temperature. The effect of this reduction in λ_L on the thermoelectric figure of merit is explored by considering a two band multivalleyed model. It is concluded that, in highly disordered, alloyed material, moderately doped and with a mean grain size of 1μm the figure of meritis increased by about 10% at 300K and about 6% at 800K compared with “single crystal” or large grain size material.     

Spatial context-based optimal multilevel energy curve thresholding for image segmentation using soft computing techniques

Neural Computing and Applications - Image segmentation using multilevel thresholding (MT) is one of the leading methods. Although, as most techniques are based on the image histogram to be...     

Goodness-of-fit testing in growth curve models: A general approach based on finite differences

Growth curve models are routinely used in various fields such as biology, ecology, demography, population dynamics, finance, econometrics, etc. to study the growth pattern of different populations and the variables linked with them. Many different kinds of growth patterns have been used in the literature to model the different types of realistic growth mechanisms. It is generally a matter of substantial benefit to the data analyst to have a reasonable idea of the nature of the growth pattern under study. As a result, goodness-of-fit tests for standard growth models are often of considerable practical value. In this paper we develop some natural goodness-of-fit tests based on finite differences of the size variables under consideration. The method is general in that it is not limited to specific parametric forms underlying the hypothesized model so long as an appropriate finite difference of some function of the size variables can be made to vanish. In addition it allows the testing process to be carried out under a set up which manages to relax most of the assumptions made by Bhattacharya et al. (2009); these assumptions are generally reasonable but not guaranteed to hold universally. Thus our proposed method has a very wide scope of application. The performance of the theory developed is illustrated numerically through several sets of real data and through simulations.     

Purification of Free DHA by Selective Esterification of Fatty Acids from Tuna Oil Catalyzed by Rhizopus oryzae Lipase

Tuna fish oil contains 25–30 % docosahexaenoic acid (DHA) and is one of the richest sources of DHA. The present paper investigates the enrichment of DHA by selective esterification of fatty acids obtained from hydrolysis of tuna fish oil catalyzed by Rhizopus oryzae lipase (ROL). The fatty acid mixture obtained after hydrolysis of tuna fish oil, referred to as tuna-FFA contained 26 % DHA. For purification/concentration of DHA in free fatty acids, selective esterification of the fatty acid mixtures with butanol was carried out using ROL in a water-organic solvent system. The best reaction parameters found in this study were pH 7, temperature 35 °C, agitation speed 800 rpm and a fatty acid to solvent (iso-octane) ratio of 1:1.32 (w/v). Also, the effects of other parameters such as type of alcohol, type of enzyme, alcohol to fatty acid ratio, enzyme to fatty acid ratio were studied to determine the most suitable reaction conditions. Exactly 76.2 % of tuna-FFA was esterified in 24 h, under the most suitable reaction conditions and the DHA content in the fatty acid fraction rose from 26 to 86.9 % with 80 % recovery of DHA, after selective esterification. The DHA content of fatty acids in butyl esters was found to be 13.6 %.     

New understandings of how dielectric properties of fruits and vegetables are affected by heat-induced dehydration: A review

Abstract

Dielectric heating (DH) is an alternative to traditional conductive heating. Preservation of fruits and vegetables through drying based on DH is faster than conventional heating systems, needing less processing time, and delivering a better dehydrated product as well as reduced treatment costs. Dielectric properties (DPs) are significant physical qualities that are affected by microwave (MW) and radio frequency (RF) heating systems; these attributes directly affect the electromagnetic arrangement and currents surrounding the materials. In other words, DPs define the response of materials in electric fields at the desired frequency and temperature. Thus, DPs of materials are of increasing interest in the agricultural and food-processing fields. The principles of the DPs of fruits and vegetables according to frequency, temperature, and composition are crucial in the designing and handling of MW and RF heating operations. A consideration of DPs is required to ensure the quality of fruits and vegetables is enhanced throughout the drying process for better quality final products. This review aimed to provide a comprehensive update on the prospects of DH for drying of fruits and vegetables. The factors that affect the DPs during the dehydration process of fruits and vegetables and discussions about the correlation among these factors were also provided. In addition, the fundamentals of DPs and their measurement techniques were also discussed. This study is an update on the state-of-the-art DH system and illustrates the important influence of DPs on the radiative heating of fruits and vegetables.     

Viability of Lactobacillus plantarum TISTR 2075 in Different Protectants during Spray Drying and Storage

Spray drying was applied for the production of Lactobacillus plantarum TISTR 2075 powder using maltodextrin as the carrier. A survival rate of 0.85% was achieved for this probiotic bacteria after spray drying. To improve the survival of this strain during the spray-drying process and storage, various protectants were added before drying. These included protein, trehalose, fibersol, ascorbic acid, isomalt, palatinose, and gum acacia. The results indicated that trehalose and protein (a combination of soy protein isolate and milk protein concentrate) significantly (P < 0.05) enhanced the viability during spray drying, with survival rates of 57.70 and 25.31%, respectively. Survival of the dried strain was also monitored over a period of 12 months’ storage at 4 and 25°C. Higher temperature induced lower viability of the strain in all protectants during this long-term storage. Accelerated storage tests using temperatures of 37, 45, 60, and 80°C were also applied to the spray-dried powders. A temperature-dependent prediction model was developed to determine the viability of the spray-dried L. plantarum TISTR 2075 in different protectants for long-term storage.     

Single step synthesis of graphene nanoribbons by catalyst particle size dependent cutting of multiwalled carbon nanotubes

Graphene nanoribbons are emerging as an interesting material for the study of low dimensional physics and for the applications in future electronics due to its finite energy band gap. However, its applicability for large scale nanoelectronics may not be effectively realized unless graphene nanoribbons could be produced using a simple, viable, cost-effective and scalable technique. Here, we report the one step facile synthesis of few layered graphene nanoribbons (GNRs) by catalytically unzipping multi-walled carbon nanotubes (MWCNTs) based on the solubility of carbon atoms in transition metals. The process is free from aggressive oxidants (such as KMnO(4), KClO(4), H(2)SO(4), HNO(3), etc.) and utilizes the in situ grown nickel nanoparticles for nanotube unzipping. This is an additional advantage over previously used techniques to synthesize GNRs. To observe the effect of catalyst particle size and reaction temperature on cutting length of the nanotubes, a simulation study has been done based on solubility of carbon atoms in metal nanoparticles.