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11.
The application and development of biopolymer-based particulate delivery systems is receiving considerable attention from scientists and manufacturers seeking to engineer healthier and safer foods. Present work focused on the fabrication and proteolysis of bovine lactoferrin nano-particles onto which pectins, alginate and carrageenan were electrostatically deposited. DLS, CD and AFM were used to ascertain the formation of spherical structures with varying zeta potentials and modified protein folding. SDS-PAGE analyses of samples collected during simulated human gastro-duodenal digestion revealed that in the case of high and low methoxy pectin particle zeta potential values (−35.4 mV and −50.3 mV respectively) were found to be inversely linked to lactoferrin digestion rate. Moreover, the electrostatic binding of food grade iota-carrageenan onto lactoferrin nano-particles was found to yield highly charged particles (zeta = −69.2 mV) with low surface roughness and to enable a proportion of the lactoferrin to resist 1 h of simulated gastric digestion. Overall, these findings suggest that electrostatic biopolymer interactions may modulate protein digestion and possibly facilitate controlled protein delivery to the upper gastrointestinal tract or have implications to protein nutritional value. 相似文献
12.
Model demonstrating the potential for coupled nitrification denitrification in soil aggregates 总被引:2,自引:0,他引:2
A model of reactive, multi-species diffusion has been developed to describe N transformations in spherical soil aggregates, emphasizing the effects of irrigation with reclaimed wastewater. Oxygen demand for respiratory activity has been shown to promote the establishment of anaerobic conditions. Aggregate size and soil respiration rate were identified as the most significant parameters governing the existence and extent of the anaerobic volume in aggregates. The inclusion of kinetic models describing mineralization, nitrification, and denitrification facilitated the investigation of coupled nitrification/denitrification (CND), subject to O2 availability. N-transformations are shown to be affected by effluent-borne NH4+-N content, in addition to elevated BOD and pH levels. Their incremental contribution to O2 availability has been found to be secondary to respiratory activity. At the aggregate level, significant differences between apparent and gross rates of N-transformations were predicted (e.g., NH4+ oxidation and N2 formation), resulting from diffusive constraints due to aggregate size. With increasing anaerobic volume, the effective nitrification rate determined at the aggregates level decreases until its contribution to nitrification is negligible. It was found that the nitrification process was predominantly limited to aggregates <0.25 cm. Assuming that nitrification is the main source for NO3- formation, denitrification efficiency is predicted to peak in medium-sized aggregates, where aerobic and anaerobic conditions coexist, supporting CND. In effluent-irrigated soils, the predicted NO2- formation rate in small aggregates is enhanced when compared to freshwater-irrigated soils. The difference vanishes with increasing aggregate size as anaerobic NO2- consumption exceeds aerobic NO2- formation due to the coupling of nitrification and denitrification. 相似文献
13.
A theory is developed for the construction of carry-save networks with minimal delay, using a given collection of carry-save adders each of which may receive inputs and produce outputs using several different representation standards.The construction of some new carry-save adders is described. Using these carry-save adders optimally, as prescribed by the above theory, we get {, , }-circuits of depth 3.48 log2
n and {, , }-circuits of depth 4.95 log2
n for the carry-save addition ofn numbers of arbitrary length. As a consequence we get multiplication circuits of the same depth. These circuits put out two numbers whose sum is the result of the multiplication. If a single output number is required then the depth of the multiplication circuits increases respectively to 4.48 log2
n and 5.95 log2
n.We also get {, , }-formulae of sizeO (n
3.13) and {, }-formulae of sizeO (n
4.57) for all the output bits of a carry-save addition ofn numbers. As a consequence we get formulae of the same size for the majority function and many other symmetric Boolean functions. 相似文献
14.
The problem of H∞ filtering of stationary discrete-time linear systems with stochastic uncertainties in the state space matrices is addressed, where the uncertainties are modeled as white noise. The relevant cost function is the expected value, with respect to the uncertain parameters, of the standard H∞ performance. A previously developed stochastic bounded real lemma is applied that results in a modified Riccati inequality. This inequality is expressed in a linear matrix inequality form whose solution provides the filter parameters. The method proposed is applied also to the case where, in addition to the stochastic uncertainty, other deterministic parameters of the system are not perfectly known and are assumed to lie in a given polytope. The problem of mixed H2/H∞ filtering for the above system is also treated. The theory developed is demonstrated by a simple tracking example. 相似文献
15.
Asaf Shabtai Author Vitae Uri Kanonov Author Vitae Author Vitae 《Journal of Systems and Software》2010,83(8):1524-1537
In this paper, a new approach for detecting previously unencountered malware targeting mobile device is proposed. In the proposed approach, time-stamped security data is continuously monitored within the target mobile device (i.e., smartphones, PDAs) and then processed by the knowledge-based temporal abstraction (KBTA) methodology. Using KBTA, continuously measured data (e.g., the number of sent SMSs) and events (e.g., software installation) are integrated with a mobile device security domain knowledge-base (i.e., an ontology for abstracting meaningful patterns from raw, time-oriented security data), to create higher level, time-oriented concepts and patterns, also known as temporal abstractions. Automatically-generated temporal abstractions are then monitored to detect suspicious temporal patterns and to issue an alert. These patterns are compatible with a set of predefined classes of malware as defined by a security expert (or the owner) employing a set of time and value constraints. The goal is to identify malicious behavior that other defensive technologies (e.g., antivirus or firewall) failed to detect. Since the abstraction derivation process is complex, the KBTA method was adapted for mobile devices that are limited in resources (i.e., CPU, memory, battery). To evaluate the proposed modified KBTA method a lightweight host-based intrusion detection system (HIDS), combined with central management capabilities for Android-based mobile phones, was developed. Evaluation results demonstrated the effectiveness of the new approach in detecting malicious applications on mobile devices (detection rate above 94% in most scenarios) and the feasibility of running such a system on mobile devices (CPU consumption was 3% on average). 相似文献
16.
Sigal Meirovitch Zvi Shtein Tal Ben-Shalom Shaul Lapidot Carmen Tamburu Xiao Hu Jonathan A. Kluge Uri Raviv David L. Kaplan Oded Shoseyov 《International journal of molecular sciences》2016,17(9)
The fabrication of cellulose-spider silk bio-nanocomposites comprised of cellulose nanocrystals (CNCs) and recombinant spider silk protein fused to a cellulose binding domain (CBD) is described. Silk-CBD successfully binds cellulose, and unlike recombinant silk alone, silk-CBD self-assembles into microfibrils even in the absence of CNCs. Silk-CBD-CNC composite sponges and films show changes in internal structure and CNC alignment related to the addition of silk-CBD. The silk-CBD sponges exhibit improved thermal and structural characteristics in comparison to control recombinant spider silk sponges. The glass transition temperature (Tg) of the silk-CBD sponge was higher than the control silk sponge and similar to native dragline spider silk fibers. Gel filtration analysis, dynamic light scattering (DLS), small angle X-ray scattering (SAXS) and cryo-transmission electron microscopy (TEM) indicated that silk-CBD, but not the recombinant silk control, formed a nematic liquid crystalline phase similar to that observed in native spider silk during the silk spinning process. Silk-CBD microfibrils spontaneously formed in solution upon ultrasonication. We suggest a model for silk-CBD assembly that implicates CBD in the central role of driving the dimerization of spider silk monomers, a process essential to the molecular assembly of spider-silk nanofibers and silk-CNC composites. 相似文献
17.
18.
Molten salt reactors (MSR) have many non-proliferation attributes. They can operate on the thorium-uranium fuel cycle which protects the fissile material by the daughter products of the inseparable U-232. MSRs can completely fission all plutonium and HEU, and as desired, ‘convert’ them to U-233. This also results in high, and efficient resource utilization, while diminishing the plutonium stock. On line processing, when applied, could free the waste from all fissile material. The fuel in the reactor stays protected by the intense radiation of the fission products. Fuel can also be protected in the reactor as well as outside the reactor by denaturing with natural uranium. A wide variety of MSRs are available, from ‘once through’ minimum processing reactors to ones with fuel processing which can breed fuel for converters. MSRs are extremely safe and simple reactors with good economic potential. 相似文献
19.
Multilayer emulsions containing lipid droplets coated by lactoferrin (LF) - anionic polysaccharide layers have improved resistance to environmental stresses (such as pH, salt, and temperature), but their behavior within the gastrointestinal tract (GIT) is currently unknown. The objective of this research was therefore to monitor changes in the physicochemical properties and digestibility of these systems under simulated GIT conditions. Primary emulsions (5% corn oil, 0.5% LF) were prepared using a high-pressure homogenizer. Secondary emulsions (5% corn oil, 0.5% LF, 0.5% polysaccharide) were prepared by incorporating alginate, low methoxyl pectin (LMP) or high methoxyl pectin (HMP) into primary emulsions. Emulsions were then subjected to simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) conditions in sequence. LF, LF-LMP and LF-HMP emulsions were stable to droplet aggregation in the stomach but aggregated in the small intestine, whereas LF-alginate emulsions aggregated in both the stomach and small intestine. The presence of a dietary fiber coating around the initial lipid droplets had little influence on the total extent of lipid digestion in SIF, but LF-alginate emulsions had a slower initial digestion rate than the other emulsions. These results suggest that the dietary fiber coatings may become detached in the small intestine, or that they were permeable to digestive enzymes. Pepsin was found to have little influence on the physical stability or digestibility of the emulsions. The knowledge obtained from this study is important for the design of delivery systems for encapsulation and release of lipophilic bioactive ingredients. 相似文献
20.
Moshe Shaked 《技术计量学》2013,55(4):457-466
Accelerated life testing for distributions with hazard rate functions of the form r(t) = Ag(t) + Bh(t) are considered. Let V 1, …, V k be stress levels larger than V 0—the stress level under normal conditions [V 0 > 0]—and let a(v) be a nondecreasing function on (0, ∞). We discuss a generalization of the common accelerated models (the power rule model and the Arrhenius model) by assuming that the hazard rate under the stress level V, is of the form (a(V t )) P (Ag(t) + Bh(t)). The maximum likelihood estimators of A, B and P for complete and censored samples are studied. The estimation procedure reduces to a solution of one equation with one unknown parameter. The estimation procedure under the assumption of aging is also described. The asymptotic variance-covariance matrix is given. 相似文献