Valentina Serra  Edoardo Fiorillo  Francesco Cucca  Valeria Orr 《International journal of molecular sciences》2023,24(1)

The use of cryopreserved peripheral blood mononuclear cells is common in biological research. It is widely accepted that primary cells are rendered unusable by several freezing cycles, although this practice might be very helpful when the biological material is valuable and its re-collection is impractical. To determine the extent to which primary cells undergoing repeated freezing cycles are comparable to one another and to fresh samples, we evaluated overall lymphocyte viability, their proliferation and cytokine production capabilities, as well as the levels of 27 cell subtypes in ten human peripheral blood mononuclear cells frozen for five years and repeatedly thawed. As expected, we observed a progressive increase in cell death percentages on three rounds of thawing, but the frequency of the main lymphocyte subsets was stable across the three thawings. Nevertheless, we observed a significant reduction of B cell frequency in frozen samples compared to fresh ones. On repeated thawings and subsequent conventional stimulation, lymphocyte proliferation significantly decreased, and IL-10, IL-6, GM-CSF, IFN-gamma, and IL-8 showed a trend to lower values.  相似文献   

    

Ilaria Andreana  Manuela Malatesta  Maria Assunta Lacavalla  Federico Boschi  Paola Milla  Valeria Bincoletto  Carlo Pellicciari  Silvia Arpicco  Barbara Stella 《International journal of molecular sciences》2023,24(1)

Muscular dystrophies are a group of rare genetic pathologies, encompassing a variety of clinical phenotypes and mechanisms of disease. Several compounds have been proposed to treat compromised muscles, but it is known that pharmacokinetics and pharmacodynamics problems could occur. To solve these issues, it has been suggested that nanocarriers could be used to allow controlled and targeted drug release. Therefore, the aim of this study was to prepare actively targeted poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) for the treatment of muscular pathologies. By taking advantage of the high affinity for carnitine of skeletal muscle cells due to the expression of Na⁺-coupled carnitine transporter (OCTN), NPs have been actively targeted via association to an amphiphilic derivative of L-carnitine. Furthermore, pentamidine, an old drug repurposed for its positive effects on myotonic dystrophy type I, was incorporated into NPs. We obtained monodispersed targeted NPs, with a mean diameter of about 100 nm and a negative zeta potential. To assess the targeting ability of the NPs, cell uptake studies were performed on C2C12 myoblasts and myotubes using confocal and transmission electron microscopy. The results showed an increased uptake of carnitine-functionalized NPs compared to nontargeted carriers in myotubes, which was probably due to the interaction with OCTN receptors occurring in large amounts in these differentiated muscle cells.  相似文献   

    

Mar Falquet  Carla Prezioso  Maria Ludvigsen  Jack-Ansgar Bruun  Sara Passerini  Baldur Sveinbjrnsson  Valeria Pietropaolo  Ugo Moens 《International journal of molecular sciences》2023,24(1)

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Vincenzo De Leo  Anna Maria Maurelli  Livia Giotta  Valeria Daniello  Sante Di Gioia  Massimo Conese  Chiara Ingrosso  Fulvio Ciriaco  Lucia Catucci 《International journal of molecular sciences》2023,24(1)

Curcumin (Cur) is a hydrophobic polyphenol from the rhizome of Curcuma spp., while hydroxytyrosol (HT) is a water-soluble polyphenol from Olea europaea. Both show outstanding antioxidant properties but suffer from scarce bioavailability and low stability in biological fluids. In this work, the co-encapsulation of Cur and HT into liposomes was realized, and the liposomal formulation was improved using polymers to increase their survival in the gastrointestinal tract. Liposomes with different compositions were formulated: Type 1, composed of phospholipids and cholesterol; Type 2, also with a PEG coating; and Type 3 providing an additional shell of Eudragit^® S100, a gastro-resistant polymer. Samples were characterized in terms of size, morphology, ζ-potential, encapsulation efficiency, and loading capacity. All samples were subjected to a simulated in vitro digestion and their stability was investigated. The Eudragit^®S100 coating demonstrated prevention of early releases of HT in the mouth and gastric phases, while the PEG shell reduced bile salts and pancreatin effects during the intestinal digestion. In vitro antioxidant activity showed a cumulative effect for Cur and HT loaded in vesicles. Finally, liposomes with HT concentrations up to 40 μM and Cur up to 4.7 μM, alone or in combination, did not show cytotoxicity against Caco-2 cells.  相似文献   

    

Pasquale Esposito MD  PhD  Alessandra Civati MD  Daniela Picciotto MD  Valeria Falqui MD  Novella Conti MD  Elisa Russo MD  Francesca Viazzi MD  PhD 《Hemodialysis international. International Symposium on Home Hemodialysis》2021,25(2):275-278

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Raffaella Di Silvestro  Alessandro Di Loreto  Sara Bosi  Valeria Bregola  Ilaria Marotti  Stefano Benedettelli  Antonio Segura‐Carretero  Giovanni Dinelli 《Journal of the science of food and agriculture》2017,97(2):641-649

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Issis Quispe‐Fuentes  Antonio Vega‐Gálvez  Valeria Vásquez  Elsa Uribe  Sebastián Astudillo 《Journal of food process engineering》2017,40(3)

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Enzo Spisni  Maria Chiara Valerii  Luigia De Fazio  Enrica Rotondo  Marcella Di Natale  Elisabetta Giovanardi  Giovanni Posabella  Valeria Bregola  Verena Stenico  Rocco Enrico Sferrazza  Giovanni Dinelli  Sara Bosi 《Journal of the science of food and agriculture》2020,100(11):4101-4107

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Jiasen Guo  George Yumnam  Ashutosh Dahal  Yiyao Chen  Valeria Lauter  Deepak K. Singh 《Advanced Electronic Materials》2021,7(9):2100079

Geometrically frustrated materials, such as spin ice or kagome lattice, are known to exhibit exotic Hall effect phenomena due to spin chirality. For the first time, Hall effect mechanism is explored in an artificial honeycomb spin ice of Nd–Sn element using Hall probe and polarized neutron reflectivity measurements. In an interesting observation, a strong enhancement in Hall signal at relatively higher temperature of T ∼ 20 K is detected. The effect is attributed to the planar Hall effect due to magnetic moment configuration in spin ice state in low field application. In the antiferromagnetic state of neodymium at low temperature, applied field induced coupling between atomic Nd moments and conduction electrons in underlying lattice causes distinct increment in Hall resistivity at very modest field of H ∼ 0.015 T. The experimental findings suggest the development of a new research vista to study the planar and the field induced Hall effects in artificial spin ice.  相似文献   

    

Miguel Ángel Rincón‐Cervera  Raúl Galleguillos‐Fernández  Valeria González‐Barriga  Rodrigo Valenzuela  Alfonso Valenzuela 《European Journal of Lipid Science and Technology》2018,120(10)

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