Efficacy of iron-fortified Ultra Rice in improving the iron status of women in Mexico

BACKGROUND: Universal fortification of staple foods with iron has been widely promoted as a cost-effective strategy to reduce iron deficiency in developing-country populations. Nonetheless, relatively few efficacy trials have been reported to date to demonstrate impact on iron status. The Ultra Rice technology provides a means of delivering fortificant iron via rice. OBJECTIVE: The objective of this study was to test the efficacy of rice fortified with microencapsulated, micronized iron pyrophosphate to improve the iron status of women in Mexico in a randomized, controlled intervention trial. METHODS: Nonpregnant, nonlactating women 18 to 49 years of age were recruited from six factories. The women received a daily portion of cooked rice 5 days per week for a period of 6 months, before and after which iron status indicators were determined in venous blood samples. RESULTS: The average intake of iron from the fortificant was 13 mg/day. Mean plasma ferritin concentration and estimated body iron stores were significantly higher, and transferrin receptors were lower, in the iron-fortified rice group following the intervention. Mean hemoglobin concentration also increased in the treatment group, but the increase was significant only when the analysis was restricted to those with baseline hemoglobin < 12.8 g/dL. The absolute reduction in anemia and iron deficiency was 10.3 and 15.1 percentage points, respectively. Total iron intake from fortificant was a significant covariate of change in body iron stores. The overall prevalence of anemia was reduced by 80%. CONCLUSIONS: Fortification of rice with iron using this technology is an efficacious strategy for preventing iron deficiency.     

The distribution of phenolic acids in pumpkin’s hull-less seed,skin, oil cake meal,dehulled kernel and hull

The phenolic acids in whole hull-less seed, its skin and oil cake meal, dehulled kernel and hull of pumpkin (Cucurbita pepo) were separated into free, esterified and insoluble-bound forms, which were then identified and quantified by high-performance liquid chromatography with a photodiode array detector. In all samples, protocatechuic, p-hydroxybenzoic, vanillic, trans-p-coumaric, ferulic, trans-sinapic acids and p-hydroxybenzaldehyde were quantified. Caffeic acid was present in all samples except in hulls, while syringic acid was not detectable only in skin and oil cake meal. p-Hydroxybenzoic acid was the dominant phenolic compound in all investigated samples, with 34.7%, 52.0%, 51.4%, 67.4% and 51.8% found in hull-less seed, oil cake meal, skin, dehulled kernels and hulls, respectively, based on total phenolic acid content. Most phenolic acids were present in bound (esterified and insoluble) form, from 50.6% in skin to 84.1% in hull-less seed.     

Influence of human gastric juice on oxidation of marine lipids – in vitro study

This study evaluates whether marine lipids can oxidise in acidic stomach environment and whether authentic gastric juice has the potential to act as a pro- or anti-oxidative medium. Oxidation of herring lipids in emulsions and liposomes was followed in in vitro digestion models containing authentic human gastric juice, and compared to models containing hydrochloric acid solution. Peroxide value, concentration of thiobarbituric acid reactive substances and oxygen uptake rate increased in all the models during 2.5 h incubation at pH 4 and 37 °C in darkness. The markers showed no difference between oxidation in gastric juice and hydrochloric acid solution. Gastric juice reduced the prooxidant activity of iron ions measured as oxygen uptake rate, but did not reduce the activity of methemoglobin. Berry juice, green tea, red wine, and caffeic acid reduced oxygen uptake in the acidic environments while coffee, ascorbic acid and orange juice increased oxidation. Beverages accompanying foods containing marine lipids will therefore affect the course of post-prandial lipid oxidation.     

A method for characterization and inter-comparison of sites with regard to solar energy utilization by statistical analysis of their solar radiation data as performed for three sites in the Israel Negev region

A method for the characterization and inter-comparison of sites with regard to their suitability for utilization of solar energy based upon the statistical analysis of their solar radiation intensities is presented. In this method each monthly data set of the daily global, horizontal beam and diffuse radiation intensities was analyzed and the following parameters were determined: monthly average daily radiation intensity, coefficient of variation, skewness and kurtosis. The values of the skewness and kurtosis have been applied, using generally accepted rules, to describe the distribution curves for each of the radiation intensity data sets. In addition, the same type of statistical analysis was applied to the monthly average daily ratios of the horizontal beam to global radiation, diffuse to global and the clearness index for the three sites. In this investigation, this statistical analysis method has been applied to the global and beam radiation measured at three sites located in the southern, Negev region of Israel, viz., Beer Sheva, Sde Boker and Eilat. The southern region of Israel is characterized by relatively high average daily irradiation intensities for both global and normal incidence radiation. They have been characterized with regard to the distribution of their intensity levels and a site inter-comparison has also been performed. An inter-comparison of the results of these analyses for the three sites has been performed on a monthly basis. The results of this analysis are used both to characterize and compare the composition of the solar radiation at the three sites under investigation. The results of this study will be presented in detail.     

Artificial Extracellular Matrices Containing Bioactive Glass Nanoparticles Promote Osteogenic Differentiation in Human Mesenchymal Stem Cells

The present study analyzes the capacity of collagen (coll)/sulfated glycosaminoglycan (sGAG)-based surface coatings containing bioactive glass nanoparticles (BGN) in promoting the osteogenic differentiation of human mesenchymal stroma cells (hMSC). Physicochemical characteristics of these coatings and their effects on proliferation and osteogenic differentiation of hMSC were investigated. BGN were stably incorporated into the artificial extracellular matrices (aECM). Oscillatory rheology showed predominantly elastic, gel-like properties of the coatings. The complex viscosity increased depending on the GAG component and was further elevated by adding BGN. BGN-containing aECM showed a release of silicon ions as well as an uptake of calcium ions. hMSC were able to proliferate on coll and coll/sGAG coatings, while cellular growth was delayed on aECM containing BGN. However, a stimulating effect of BGN on ALP activity and calcium deposition was shown. Furthermore, a synergistic effect of sGAG and BGN was found for some donors. Our findings demonstrated the promising potential of aECM and BGN combinations in promoting bone regeneration. Still, future work is required to further optimize the BGN/aECM combination for increasing its combined osteogenic effect.     

基于虚拟变形法的移动质量识别方法研究

在车-桥耦合系统的移动质量(荷载)识别反问题中,识别移动质量会面临重构系统、优化缓慢的问题;而若直接识别移动荷载常常会遇到病态问题且对噪音敏感。针对这些缺陷,根据虚拟变形法(VDM)的结构快速重分析思想,提出移动动态影响矩阵,实现利用较少的传感器即可快速而准确地识别移动质量(荷载)。以移动质量为优化变量,避免了识别荷载常遇到的病态问题,对噪音鲁棒性强;且需要传感器信息少。每步优化中,利用移动动态影响矩阵,无需时时重构车-桥耦合系统的时变系统参数矩阵,优化效率高。VDM方法的思想是将实际结构的响应计算转化为初始结构模型在相同外荷载作用下的响应,与在结构模型发生改变的位置施加相关的虚拟变形或虚拟力引起的响应的线性叠加。通过简支梁模型和框架梁模型验证了该方法的可行性和有效性,即使在5%的噪声影响下,利用一个传感器就可以很好地识别多个移动质量。     

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-inducible promoters of B. subtilis: the SOS promoter PdinC, the methylation-specific response promoter PalkA, and the oxidative stress promoter PmrgA. The luminescence of B. subtilis-based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and H₂O₂. Comparison with Escherichia coli-based lux-biosensors, where the promoters PdinI, PalkA, and Pdps were used, showed generally similar characteristics. However, for B. subtilis PdinC, a higher response amplitude was observed, and for B. subtilis PalkA, on the contrary, both the amplitude and the range of detectable toxicant concentrations were decreased. B. subtilis PdinC and B. subtilis PmrgA showed increased sensitivity to the genotoxic effects of the 2,2′-bis(bicyclo [2.2.1] heptane) compound, which is a promising propellant, compared to E. coli-based lux-biosensors. The obtained biosensors are applicable for detection of toxicants introduced into soil. Such bacillary biosensors can be used to study the differences in the mechanisms of toxicity against Gram-positive and Gram-negative bacteria.