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201.
This study focused on analyzing active pathways and the metabolic flux distribution in human neuronal AGE1.HN cells that is a desirable basis for a rational design and optimization of producing cell lines and production processes for biopharmaceuticals. 13C-labeling experiments and 13C metabolic flux analysis were conducted using glucose, glutamine, alanine and lactate tracers in parallel experiments. Connections between cytosolic and mitochondrial metabolite pools were verified, e.g., flux from TCA cycle metabolite 13C to glycolytic metabolites. It was also found that lactate and alanine are produced from the same pyruvate pool and that consumed alanine is mainly directly metabolized and secreted as lactate. Activity of the pentose phosphate pathway was low being around 2.3% of the glucose uptake flux. This might be compensated in AGE1.HN by high mitochondrial malic enzyme flux producing NADPH. Mitochondrial pyruvate transport was almost zero. Instead pyruvate carbons were channeled via oxaloacetate into the TCA cycle which was mainly fed via α-ketoglutarate and oxaloacetate during the investigated phase. The data indicate that further optimization of this cell line should focus on the improved substrate usage which can be accomplished by an improved connectivity between glycolytic and mitochondrial pyruvate pools or by better control of the substrate uptake. 相似文献
202.
Second generation CIS solar modules 总被引:1,自引:0,他引:1
Multinary Cu(In,Ga)(Se,S)2 absorbers (abrev. CIGSSe) are promising candidates for reducing the cost of photovoltaics well below the cost of crystalline silicon. Shell Solar has pioneered production of this new thin film technology and is now with the first generation at a volume of well over 1 MW/year. In a separate pilot line for second generation products we have further improved the performance of CIGSSE based solar modules. We developed a novel CIGSSE formation technique called stacked elemental layer rapid thermal processing (SEL-RTP). This process has recently been scaled up from initial laboratory sized mini-modules (10 × 10 cm2) to full sized power modules of 60 × 90 cm2. The present paper concentrates on in situ and ex situ characterization techniques that were developed to control and further improve large area CIGSSE processing. The crystalline thin film formation process has been analyzed with in situ thin film calorimetry and in situ X-ray diffraction (XRD). That work has added fundamental insights and accelerates the optimization process. The depth distribution of gallium and sulfur has been determined by secondary ion mass spectroscopy (SIMS) for different selenization and sulfurization processes. Appropriate profiles of these elements allow for a deliberate bandgap profiling within the Cu(In,Ga)(S,Se)2 absorber. In addition further quality control tools like X-ray fluorescence analysis and Raman spectroscopy for stoichiometry monitoring, photoluminescence lifetime mapping and thermographic imaging have been developed in order to improve large area uniformity and reproducibility.
Some first full sized modules from the new pilot line look very promising: Aperture area efficiencies of up to 13.1% for monolithic thin film circuits on 0.54 m2 and a module power of 65 W represent an international champion value for large are thin film solar modules. 相似文献
203.
Matthias A. Ruderer Shuai Guo Robert Meier Hsin‐Yin Chiang Volker Körstgens Johannes Wiedersich Jan Perlich Stephan V. Roth Peter Müller‐Buschbaum 《Advanced functional materials》2011,21(17):3382-3391
Studies on the influence of four different solvents on the morphology and photovoltaic performance of bulk‐heterojunction films made of poly(3‐hexylthiophene) (P3HT) and [6,6]‐phenyl‐C61 butyric acid methyl ester (PCBM) via spin‐coating for photovoltaic applications are reported. Solvent‐dependent PCBM cluster formation and P3HT crystallization during thermal annealing are investigated with optical microscopy and grazing‐incidence wide‐angle X‐ray scattering (GIWAXS) and are found to be insufficient to explain the differences in device performance. A combination of atomic force microscopy (AFM), X‐ray reflectivity (XRR), and grazing‐incidence small‐angle X‐ray scattering (GISAXS) investigations results in detailed knowledge of the inner film morphology of P3HT:PCBM films. Vertical and lateral phase separation occurs during spin‐coating and annealing, depending on the solvent used. The findings are summarized in schematics and compared with the IV characteristics. The main influence on the photovoltaic performance arises from the vertical material composition and the existence of lateral phase separation fitting to the exciton diffusion length. Absorption and photoluminescence measurements complement the structural analysis. 相似文献
204.
Nuclear magnetic resonance (NMR) spectroscopy is widely used as an analytical platform for metabolomics. Many studies make use of 1D spectra, which have the advantages of relative simplicity and rapid acquisition times. The spectral data can then be analyzed either with a chemometric workflow or by an initial deconvolution or fitting step to generate a list of identified metabolites and associated sample concentrations. Various software tools exist to simplify the fitting process, but at least for 1D spectra, this still requires a degree of skilled operator input. It is of critical importance that we know how much person-to-person variability affects the results, in order to be able to judge between different studies. Here we tested a commercially available software package (Chenomx' NMR Suite) for fitting metabolites to a set of NMR spectra of yeast extracts and compared the output of five different people for both metabolite identification and quantitation. An initial comparison showed good agreement for a restricted set of common metabolites with characteristic well-resolved resonances but wide divergence in the overall identities and number of compounds fitted; refitting according to an agreed set of metabolites and spectral processing approach increased the total number of metabolites fitted but did not dramatically increase the quality of the metabolites that could be fitted without prior knowledge about peak identity. Hence, robust peak assignments are required in advance of manual deconvolution, when the widest range of metabolites is desired. However, very low concentration metabolites still had high coefficients of variation even with shared information on peak assignment. Overall, the effect of the person was less than the experimental group (in this case, sampling method) for almost all of the metabolites. 相似文献
205.
Trace theory has been developed to describe the behavior of concurrent systems. Synchronization of traces is of special interest for a modular approach. We characterize those trace monoids for which synchronization can be described locally. 相似文献
206.
207.
EA Vagner VA Bruns OT Artemov AV Kasatov MV Altyntsev 《Canadian Metallurgical Quarterly》1998,(9):62-64
During 20 years (1972-1994) the authors carried out proximal resections of the stomach in 106 patients for cancer of proximal part of the stomach extended to the esophagus. Morphological verification data are presented. Total lethality rate made up 20.8%. It decreased with gaining of experience and improvement in selection of patients (1972-1982--29.6%, 1983-1994--17.7%). The most common complication in postoperative period was exudative pleuritis: in 16% of operated patients insufficiency of sutures of the esophagogastric anastomosis was observed. For prophylaxis of suture insufficiency pyloroplasty by Heineke-Mikulich and continuous (to 4-5 days) naso-gastral drainage were of principal significance. Great importance was attached to establishment of the anastomosis by the method developed by the authors. The results of the operations depend also on the operative approach: two-stage combined approach (laparotomy, thoracotomy) brings the least lethality. 相似文献
208.
209.
Concentrating solar thermal power and photovoltaics are two major technologies for converting sunlight to electricity. Variations of the annual solar irradiation depending on the site influence their annual efficiency, specific output and electricity generation cost. Detailed technical and economical analyses performed with computer simulations point out differences of solar thermal parabolic trough power plants, non-tracked and two-axis-tracked PV systems. Therefore, 61 sites in Europe and North Africa covering a global annual irradiation range from 923 to 2438 kW h/m2 a have been examined. Simulation results are usable irradiation by the systems, specific annual system output and levelled electricity cost. Cost assumptions are made for today's cost and expected cost in 10 years considering different progress ratios. This will lead to a cost reduction by 50% for PV systems and by 40% for solar thermal power plants. The simulation results show where are optimal regions for installing solar thermal trough and tracked PV systems in comparison to non-tracked PV. For low irradiation values the annual output of solar thermal systems is much lower than of PV systems. On the other hand, for high irradiations solar thermal systems provide the best-cost solution even when considering higher cost reduction factors for PV in the next decade. Electricity generation cost much below 10 Eurocents per kW h for solar thermal systems and about 12 Eurocents/kW h for PV can be expected in 10 years in North Africa. 相似文献
210.
Support Vector Machines for 3D Shape Processing 总被引:7,自引:0,他引:7