Current Scenario of Adsorbent Materials Used in Ethylene Scavenging Systems to Extend Fruit and Vegetable Postharvest Life

Fruit and vegetables are much appreciated by consumers due to their nutritional values and health-promoting compounds. However, different factors affect the postharvest life of such products, in where ethylene is a major one, even at low concentrations, besides temperature and relative humidity. Therefore, high attention has been focused on the development of effective tools to remove ethylene from the atmosphere surrounding these products during storage or in transit. Potassium permanganate scrubbers are one of the most used technologies to remove ethylene from horticultural products. To facilitate and improve the oxidation process, potassium permanganate has been supported onto inert solid materials of a small particle size. In this review, we aim to provide an outline of the most common materials used as potassium permanganate supports on postharvest treatment and their respective effects on quality aspects of various fresh produce during postharvest life. Vermiculite, activated alumina, zeolite, silica gel, activated carbon and clays are the most popular materials that have been used as a support of potassium permanganate-based ethylene scrubbers. The literature suggests that potassium permanganate supported onto silica gel or zeolite seems to be a promising tool to maintain fruit and vegetables quality attributes for long-term storage. Although vermiculite and activated alumina are the most commonly used materials to reach this goal, not promising results have been reported.     

Effects of <Superscript>60</Superscript>Co-γ and Electron Beam Irradiation on Storage Quality of <Emphasis Type="Italic">Panax ginseng</Emphasis>

Fresh ginseng was irradiated with ⁶⁰Co-γ or an electron beam at the dose of 2 and 4 kGy and stored at 2 °C. The quality of ginseng was evaluated after 60 and 120 days. Results suggest that when the storage time was 120 days, 2 kGy EI-treated ginseng had the lowest decay rate. During the 2 storage periods, the amounts of most of 9 ginsenosides decreased with an increase in the irradiation dose. The total saponin content of both ⁶⁰Co-γ-irradiated and EI-treated samples decreased with an increase in the irradiation dose. Significant (P <?0.05) upregulation of total polysaccharide content was observed in the 4 kGy EI samples after storage. Samples irradiated at 2 or 4 kGy by electron beam irradiation had the highest concentration of total saponin and ginseng polysaccharides, respectively. Levels of most of 17 amino acids decreased with the increasing irradiation dose. Exposure to irradiation doses up to 4 kGy affected the amino acid content of fresh ginseng. Ginseng irradiated at 4 kGy by ⁶⁰Co-γ irradiation and stored for 120 days had a significantly higher activity of superoxide dismutase (P <?0.05). A significant difference was observed in the total concentration of nucleosides and nucleobases between irradiated and un-irradiated ginseng samples (P <?0.05). These data suggest that the combination of a lower irradiation dose and low temperature is an optimal condition for extending the shelf-life of fresh ginseng without detriment to its ingredients.     

Use of an Online Extraction Technique Coupled to Liquid Chromatography for Determination of Caffeine in Coffee,Tea, and Cocoa

Caffeine is the most widely studied psychoactive molecule in history due to its many pharmacological activities and a high number of biological and physiological effects. In literature, there is a great number of applications that describe extraction, identification, and quantification of caffeine in foods and beverages. For this purpose, an extraction step is followed by an analytical technique for the identification and quantification of caffeine. This work proposes an innovative method in which sample preparation, separation, and detection steps are unified in a single step. To the best of our knowledge, this is the first report on the determination of caffeine in coffee, tea, and cocoa by means of an online extraction coupled to a liquid chromatographic system equipped with a photodiode array detector. The developed methodology was validated in terms of sensitivity, detection limits, accuracy, and precision. The advantages of this technique are (i) a significant reduction of analysis time (more than 70%) and of solvents used (the extraction step is integrated in the chromatographic analysis), (ii) the whole procedure is thus completely automated drastically reducing possible operator errors to occur, and (iii) easily realized by using a conventional monodimensional liquid chromatography system.     

In-House Validation and Comparison of Two Wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis>) Taxon-Specific Real-Time PCR Methods for GMO Quantification Supported by Droplet Digital PCR

Common wheat is one of the most important staple food crops worldwide. However, unlike other important staple crops such as maize or soybean, genetically modified (GM) wheat is not yet present in the global food market. Nonetheless, in the recent past, the adventitious presence of GM glyphosate-tolerant volunteers was reported in open wheat fields in the USA. The European Union Reference Laboratory for GM Food and Feed (EURL-GMFF) was therefore called to develop a strategy to detect such unauthorised GM wheat in wheat samples by using both taxon-specific and screening tests. Two candidate common wheat taxon-specific real-time PCR methods were suggested, one targeting ssII-D gene coding for starch synthase and the other targeting waxy-D1 gene, coding for granule-bound starch synthase. In the present study, the two above-mentioned real-time PCR taxon-specific methods were in-house verified and compared, proposing droplet digital PCR (ddPCR) as a new tool for supporting the application of the European Network of GMO Laboratories (ENGL) established method performance criteria. Preliminary performance data of waxy-D1 and ssII-D methods in ddPCR format are shown too to give a contribution to the bridging process from the consolidated to the emerging quantitative PCR methodology.     

Etablierung eines Frühwarnsystems zur Erkennung lebensmittelbedingter Risiken in Bayern – risikoorientierte Lebensmittelüberwachung weiter gefasst

Recent and past food scandals highlight the urgent need for food safety authorities to anticipate future risks in order to enable improved and proactive response mechanisms. With this goal in mind, the Bavarian Health and Food Safety Authority (LGL) has established an early warning system aimed at early identification of potential health risks and fraudulent practices in the food sector. The early warning approach allows intervention at different stages of risk development. On the one hand, the scanning of a broad area of the food production chain (horizon scanning) may enable prospective risk identification. To this end, relevant factors of influence (drivers) need to be identified, observed and analyzed. On the other hand, the retrospective analysis of case studies may unravel novel cause-effect associations that could be transferred to other products and production technologies, possibly leading to the identification of new drivers (root cause analysis). Additionally, various datasets emanating from food safety authorities, for example data from the European Rapid Alert System for Food and Feed (RASFF) and from Bavarian-wide laboratory analyses and food business inspections, will be closely observed and analyzed. The multidisciplinary character of the LGL has proven to be essential for the realization of the early warning approach, the analysis of inflowing data and implementation of consequences such as food business inspections and sampling for analysis. This article details the objectives and methodical approaches of the project. The acquisition of systematically compiled information as well as its analysis and assessment are comprehensively described. The early warning system presented here has already contributed significantly to the risk-oriented food inspection practiced to date, by integrating novel risk-relevant aspects.     

Residues of lead,cadmium, mercury and tin in canned meat products from Egypt: an emphasis on permissible limits and sources of contamination

In an attempt to determine the residual levels of lead (Pb), cadmium (Cd), mercury (Hg) and tin (Sn) in canned meat products marketed in Egypt, a total number of 160 random samples (40 each) of canned chicken luncheon (CCL), canned beef luncheon (CBL), canned frankfurter (CF) and canned corned beef (CCB) were randomly collected from different supermarkets in Egypt to be analyzed using atomic absorption spectrophotometry. From the obtained results, it was found that the mean values of residual levels of Pb in examined CCL, CBL, CF and CCB samples were 0.330, 0.224, 0.206 and 0.334 mg/kg, respectively, while those of Cd were 0.057, 0.053, 0.039 and 0.042 mg/kg, those of Hg were 0.387, 0.450, 0.402 and 0.332 mg/kg, and finally those of Sn were 2.061, 2.308, 0.755 and 1.997 mg/kg. The obtained results were compared with the permissible limits of heavy metals recommended by international and national authorities. In addition, the public health significance as well as the sources of contamination of canned meat products by heavy metals were addressed.     

Nutritional,functional, thermal and structural characteristics of <Emphasis Type="Italic">Citrullus lanatus</Emphasis> and <Emphasis Type="Italic">Limonia acidissima</Emphasis> seed flours

Seeds from fruits such as Citrullus (C.) lanatus (watermelon) and Limonia (L.) acidissima (wood apple) are not commonly utilized but could be suitable in numerous food formulations. It was shown that the protein content of defatted seed flours was 71.38 and 49.51 % and that these contained considerable amounts of minerals such as Na, Mn, Mg, K, Cu, Fe and Zn. The defatted L. acidissima seed flour was superior to C. lanatus in essential amino acids. The flours obtained from both seeds were also evaluated for functional properties and characterized by X-ray diffraction, differential scanning calorimeter and scanning electron microscope (SEM). Amorphous nature was observed in defatted C. lanatus and L. acidissima flours due to the low percentage of degree of crystallinity. Spherical morphologies were observed through SEM. The exothermic peak was recorded in defatted C. lanatus and L. acidissima flour.     

Rheological characterization of konjac glucomannan in concentrated solutions

Dynamic viscoelasticity measurements were performed for concentrated solutions of konjac glucomannan in an ionic liquid. The entanglement coupling appeared in the rheological data for each solution was characterized in terms of the molecular weight between entanglements (M _e) as an average size of the transient entanglement network. The value of M _e for konjac glucomannan in the molten state was estimated to be 1.8 × 10³ (in g mol^?1), being significantly smaller than that for cellulose, although the molecular weight and linkage of the repeating units were the same between these polysaccharides. This result suggested that the configuration of the repeating monosaccharide unit affected the entanglement network of these polysaccharides reflecting the single chain characteristics.     

Characterization of hot-air drying and infrared drying of spearmint (<Emphasis Type="Italic">Mentha spicata</Emphasis> L.) leaves

In this study, spearmint leaves were dried using hot-air (HA) and infrared (IR) techniques. Dried products were comprehensively analyzed for their drying time, specific energy consumption (SEC), rehydration, essential oil content and color changes. The IR drying process was carried out at radiation intensity levels of 1562, 3125 and 4688 W/m², emitter to sample distances of 10, 15 and 20 cm and air temperatures of 30, 40 and 50 °C. For HA drying, three levels of air temperature (30, 40 and 50 °C) and air velocity (0.5, 1 and 1.5 m/s) were applied. The results indicated that drying time, SEC and color changes were lower in IR drying of spearmint than in HA drying. IR drying gave the highest essential oil content (0.95 %) and rehydration ratio (0.788 kg water/kg dry matter (DM)). Totally, it was observed that IR drying of spearmint resulted in better quality preservation and had lower energy costs in comparison to HA drying.     

Effects of Ultrasound on Polyphenol Retention in Apples After the Application of Predrying Treatments in Liquid Medium

The effect of sonication time and kind of liquid medium on polyphenol retention and microstructure changes during predrying treatment of apple tissue was investigated. The apple cubes from ‘Idared’ cultivar were submerged in water or sucrose solution and sonicated indirectly in beakers placed in a water bath fitted with ultrasound transducers (25 kHz, 0.1 W/cm³) at 40 °C. The treatment was conducted with and without ultrasound applied for 45 and 90 min. The content of individual polyphenols was monitored by a reverse-phase high-performance liquid chromatography (RP-HPLC) method. The dominant phenolic compounds in apple were procyanidins, accounting for 56 % of total polyphenols. While a significant effect of sonication on mass transfer intensification was observed when the samples were dehydrated in sucrose solution, almost no negative effects of ultrasound application were perceived on polyphenols concentration, except for dimers of catechin. When using ultrasound in water solution, an increase in polyphenol compound losses was noted. Furthermore, the ultrasound energy caused an apple tissue structure modification which additionally affected polyphenol retention during predrying treatment.