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61.
PURPOSE: The phenoxyacetic acid, ethacrynic acid (ECA), has potential use in glaucoma therapy because it acts to increase aqueous outflow in vivo and in vitro. In human trabecular meshwork (HTM) cell culture, ECA acts to change cell shape and attachment, effects that have been correlated with microtubule (MT) alterations and chemical sulfhydryl (SH) reactivity. To further explore these actions, we evaluated two non-SH reactive phenoxyacetic acids, inadcrinone and ticrynafen, and the MT-disrupting drug vinblastine. METHODS: Excised bovine and porcine eyes were perfused and outflow facility measured. Calf pulmonary artery endothelial and HTM cells were grown in culture and cytoskeletal effects evaluated after drug treatment. RESULTS: Indacrinone, ticrynafen, and vinblastine all caused an increase in outflow facility. In contrast with ECA, the outflow effects of indacrinone and ticrynafen were not blocked by excess cysteine. Although indacrinone and ticrynafen produced changes in cell shape in vitro, the beta-tubulin staining pattern of treated cells was not altered. Vinblastine caused cell shape change and the expected MT disruption. CONCLUSIONS: Phenoxyacetic acids can increase aqueous outflow facility and alter HTM cell shape and attachment in vitro by a non-SH, non-MT mechanism (which is probably shared also by ECA). These findings suggest the possibility of a broader class of glaucoma drugs that may be directed at the HTM. An understanding of the cellular target for these drugs has implications both for potential glaucoma therapy and for the cytoskeletal mechanisms involved in normal outflow function. 相似文献
62.
The ALCHEMI method for locating the sites of foreign atoms within crystals is known to be sensitive to the delocalized emission of X-rays. This can result in large errors in some cases through differences in delocalization for different excitations or by error amplification in the ratio method of analysis. An alternative approach to the analysis of ALCHEMI data, using multivariate statistical analysis, is extended to the case of multiple impurities. Initial results from zone-axis channelling experiments for a Yb-doped zirconolite (CaZrTi2O7) are shown to confirm the improved accuracy of this method, especially for axial orientations. Data were collected using a 400-keV analytical electron microscope fitted with an intrinsic Ge X-ray detector. The potential advantages for ALCHEMI analysis of Ge detectors are considered. 相似文献
63.
For pt.I see ibid., vol.39, no.3, p.258-67 (1992). The winding of fiber optic filament in the hoop, or precision, pattern is considered. Various automatic control options that have been designed and tested are described. The controllers are designed to regulate the fiber lag angle, which is the angle at which the feed filament approaches the take-up spool. Successful, flawless winding is directly related to accurate lag regulation. The contributions are distinguished by the fact that the automatic winding rate is 10 to 30 times greater than that achieved before by operator-assisted winding 相似文献
64.
One of the most complicated ANN models, the neocognitron (NC), is adapted to an efficient all-digital implementation for VLSI. The new model, the digi-neocognitron (DNC), has the same pattern recognition performance as the NC. The DNC model is derived from the NC model by a combination of preprocessing approximation and the definition of new model functions, e.g., multiplication and division are eliminated by conversion of factors to powers of 2, requiring only shift operations. The NC model is reviewed, the DNC model is presented, a methodology to convert NC models to DNC models is discussed, and the performances of the two models are compared on a character recognition example. The DNC model has substantial advantages over the NC model for VLSI implementation. The area-delay product is improved by two to three orders of magnitude, and I/O and memory requirements are reduced by representation of weights with 3 bits or less and neuron outputs with 4 bits or 7 bits. 相似文献
65.
For heat treating cylindrical metal billets, their lay-out in a furnace usually resembles that of an in-line heat-exchanger tube bank, with the air flows orthogonal to the billets. For maximum effectiveness of transferring thermal energy from the forced convecting air to the billets, the optimal horizontal pitch-to-diameter ratio for the billets should be 1·33 ± 0·05. For this same purpose it is advantageous to employ a vertical spacing (between the billets) as large as can be permitted for a given load of billets. 相似文献
66.
The conditioned cue preference (CCP) task was used to study the information required to discriminate between spatial locations defined by adjacent arms of an 8-arm radial maze. Normal rats learned the discrimination after 3 unreinforced preexposure (PE) sessions and 4 food paired-unpaired training trials. Fimbria-fornix lesions made before, but not after, PE, and hippocampus lesions made at either time, blocked the discrimination, suggesting that the 2 structures processed different information. Lateral amygdala lesions made before PE facilitated the discrimination. This amygdala-mediated interference with the discrimination was the result of a conditioned approach response that did not discriminate between the 2 arm locations. A hippocampus/fimbria-fornix system and an amygdala system process different information about the same learning situation simultaneously and in parallel. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
67.
68.
HA Mook DM Paul BC Sales LA Boatner L Cussen 《Canadian Metallurgical Quarterly》1988,38(16):12008-12010
69.
70.
W J Cole P J Eppard B G Boysen K S Madsen R H Sorbet M A Miller R L Hintz T C White W E Ribelin B G Hammond 《Journal of dairy science》1992,75(1):111-123
Eighty-two lactating Holstein cows in their first, second, or third lactation received either one, three, or five concurrent i.m. injections of a unit dose (.6 g) of zinc methionyl bST (sometribove) or five doses of the vehicle. Injections were given at 14-d intervals from 60 +/- 3 d postpartum until the end of lactation or necropsy. Thirty-eight animals were continued on treatment for a 2nd yr. Sometribove did not affect the incidence of ketosis, milk fever, tetany, or pneumonia. Digestive disorders, primarily cows going off feed, were increased by bST during yr 1 only. The incidence of lameness was increased by bST in some time frames because of an increase in the 3.0-g bST group. Lameness was not associated with treatment-specific histopathologic changes or with abnormalities in cartilage or bone. Reproductive health generally was unaffected by treatment, but delayed conception and increased incidence of abortion were noted. Incidence of cystic ovaries was unaffected by bST. Pregnancy rates were decreased during the 100-d breeding interval of yr 1 but not during the 215-d interval of yr 2. The incidence of clinical mastitis was increased by bST, primarily at the 3.0-g dose. During the 2-yr study, 0, 3, 3, and 2 cows died or became moribund on 0, .6, 1.8, and 3.0 g of bST, respectively. Health issues identified for further evaluation included lameness and clinical mastitis for the 3.0-g group and early removal from the herd and decreased reproductive performance for all bST groups. Bovine somatotropin caused no treatment-specific toxic effects in dairy cows even at 3.0 g every 14 d. 相似文献