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排序方式: 共有622条查询结果,搜索用时 15 毫秒
31.
32.
de Boer AR Letzel T van Elswijk DA Lingeman H Niessen WM Irth H 《Analytical chemistry》2004,76(11):3155-3161
Liquid chromatography (LC) was coupled on-line to a continuous-flow enzymatic assay using electrospray ionization mass spectrometry (ESI-MS) as readout for the screening of enzyme inhibitors in complex samples. Inhibitors were detected by changes in the concentration of the enzymatic reaction products, indicating the inhibition of enzymatic activity. The molecular masses of the inhibitors were determined with high certainty by using retention time matching and peak shape comparison. Due to the high matching accuracy, baseline separation of coeluting analytes was not necessary in order to identify the correct masses of the bioactive compounds. The continuous-flow system was successfully applied for the screening of complex samples, such as natural extracts. For a red clover extract, detection limits of 0.3-0.8 micromol/L were obtained. System validation was performed by determining the IC(50) values of four inhibitors in the flow-injection mode. The IC(50) values were in the 0.11-5.6 micromol/L range and correspond closely to data obtained by microtiter plate assays. Detection limits were in the range of 0.018-0.35 micromol/L in the flow-injection mode, and 0.075-0.75 micromol/L in the LC mode. These values are well below the typical compound concentrations (1-10 micromol/L) used in high-throughput screening. Together with an interday precision of 12.6%, these results demonstrate the applicability of the system for bioactivity screening of complex mixtures, generating both chemical and biological information on bioactive compounds in a single run. 相似文献
33.
In this paper, we propose a robust wavelet domain method for noise filtering in medical images. The proposed method adapts itself to various types of image noise as well as to the preference of the medical expert; a single parameter can be used to balance the preservation of (expert-dependent) relevant details against the degree of noise reduction. The algorithm exploits generally valid knowledge about the correlation of significant image features across the resolution scales to perform a preliminary coefficient classification. This preliminary coefficient classification is used to empirically estimate the statistical distributions of the coefficients that represent useful image features on the one hand and mainly noise on the other. The adaptation to the spatial context in the image is achieved by using a wavelet domain indicator of the local spatial activity. The proposed method is of low complexity, both in its implementation and execution time. The results demonstrate its usefulness for noise suppression in medical ultrasound and magnetic resonance imaging. In these applications, the proposed method clearly outperforms single-resolution spatially adaptive algorithms, in terms of quantitative performance measures as well as in terms of visual quality of the images. 相似文献
34.
This paper presents a new algorithm for enhancement of microcalcifications in mammograms. The main novelty is the application of techniques we have developed for construction of filterbanks derived from the continuous wavelet transform. These discrete wavelet decompositions, called integrated wavelets, are optimally designed for enhancement of multiscale structures in images. Furthermore, we use a model based approach to refine existing methods for general enhancement of mammograms resulting in a more specific enhancement of microcalcifications. We present results of our method and compare them with known algorithms. Finally, we want to indicate how these techniques can also be applied to the detection of microcalcifications. Our algorithm was positively evaluated in a clinical study. It has been implemented in a mammography workstation designed for soft-copy reading of digital mammograms developed by IMAGETOOL, Germany. 相似文献
35.
Hoffmann M Küppers H Schneller T Böttger U Schnakenberg U Mokwa W Waser R 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2003,50(10):1240-1246
High piezoelectric coupling coefficients of PZT-based material systems can be employed for actuator functions in micro-electro-mechanical systems (MEMS) offering displacements and forces which outperform standard solutions. This paper presents simulation, fabrication, and development results of a stress-compensated, PZT-coated cantilever concept in which a silicon bulk micromachining process is used in combination with a chemical solution deposition (CSD) technique. Due to an analytical approach and a finite element method (FEM) simulation for a tip displacement of 10 /spl mu/m, the actuator was designed with a cantilever length of 300 /spl mu/m to 1000 /spl mu/m. Special attention was given to the Zr/Ti ratio of the PZT thin films to obtain a high piezoelectric coefficient. For first characterizations X-ray diffraction (XRD), scanning electron microscopy (SEM), hysteresis-, current-voltage I(V)and capacitance-voltage C(V)-measurements were carried out. 相似文献
36.
This paper presents a simulation-based planning software for high complexity production settings which currently undergoes first real-data industry application tests in commercial vehicle assembly. The system will also be applicable in other industries with a sufficiently high number of products and variants. The solution enables the forecasting of required personnel and temporary additional workers (“floaters”) for every cycle at the production line. It periodically collects process times, sequence and feature data for the vehicles. The cumulated workload is calculated under consideration of flexibilities. A web-based user interface enables to set and change reference data, define process simulations and to compile diagrams and charts for the analysis of the results of the different simulation runs. Various filters as to time periods, vehicle types or feature variants allow the planner a target-oriented analysis and provide feedback for improving production line tacting. Finally, some qualitative results of a first industry test in commercial vehicle assembly give feedback about the usability of existing analytical features and desirable additional features. 相似文献
37.
This study investigated whether compatibility between responses and their consistent sensorial effects influences performance in manual choice reaction tasks. In Experiment 1 responses to the nonspatial stimulus attribute of color were affected by the correspondence between the location of responses and the location of their visual effects. In Experiment 2, a comparable influence was found with nonspatial responses of varying force and nonspatial response effects of varying auditory intensity. Experiment 3 ruled out the hypothesis that acquired stimulus-effect associations may account for this influence of response-effect compatibility. In sum the results show that forthcoming response effects influence response selection as if these effects were already sensorially present, suggesting that in line with the classical ideomotor theory, anticipated response effects play a substantial role in response selection. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
38.
Wilfried Roetzel 《化学工程与技术》1989,12(1):379-387
An experimental technique and evaluation method is described for the determination of local heat transfer coefficients in tubes or other ducts. By means of a rotary mixing valve, cold and warm fluid flows are mixed in order to generate an arbitrarily shaped but periodically oscillating inlet temperature profile at the test tube. The propagation of the fundamental harmonic oscillation from the fluid to the outer surface of the tube wall is calculated analytically. Comparison of fluid and wall oscillations yields the heat transfer coefficient to be measured. The inaccurate measurement of fluid bulk temperature in the centre of the cross-section is compensated by an additional correction. Experiments were carried out with turbulent water flow through a copper tube. Measured heat transfer coefficients were compared to values calculated using Hausen's equation and good agreement was obtained. 相似文献
39.
Slobodanka Dana Kathrin Tomic Domagoj Drenjanac Goran Lazendic Sandra Hörmann Franz Handler Wilfried Wöber Christian Aschauer Wolfgang Auer 《e & i Elektrotechnik und Informationstechnik》2014,131(7):223-229
Optimization of production processes in agriculture requires that innovation in form of efficiency enhancing devices is being continuously introduced and integrated within the production environments. This paper describes how semantic modelling, web services and automatic logical reasoning are used as system enablers for such value-adding integration in implementation of semantic-based diagnoses supporting optimization of milk production processes of dairy farms. 相似文献
40.
Intermittent Switching Between Potential Flow and Turbulence in Superfluid Helium at mK Temperatures
Michael Niemetz Hubert Kerscher Wilfried Schoepe 《Journal of Low Temperature Physics》2002,126(1-2):287-296
Superfluid flow around an oscillating microsphere is investigated at temperatures down to 25 mK. Stable laminar flow below a critical velocity and turbulence at large drives are found to be separated below 0.5 K by an intermediate range of driving forces where the flow is unstable, intermittently switching between laminar and turbulent phases. We have recorded time series of this switching phenomenon and have made a statistical analysis of the switching probability. The mean lifetime of the turbulent phases grows with increasing drive and becomes infinite at a critical value. Stability of the laminar phases above the critical velocity is limited by natural background radioactivity or cosmic rays. 相似文献