Cysteine tagging for MS-based proteomics

Amino acid-tagging strategies are widespread in proteomics. Because of the central role of mass spectrometry (MS) as a detection technique in protein sciences, the term "mass tagging" was coined to describe the attachment of a label, which serves MS analysis and/or adds analytical value to the measurements. These so-called mass tags can be used for separation, enrichment, detection, and quantitation of peptides and proteins. In this context, cysteine is a frequent target for modifications because the thiol function can react specifically by nucleophilic substitution or addition. Furthermore, cysteines present natural modifications of biological importance and a low occurrence in the proteome that justify the development of strategies to specifically target them in peptides or proteins. In the present review, the mass-tagging methods directed to cysteine residues are comprehensively discussed, and the advantages and drawbacks of these strategies are addressed. Some concrete applications are given to underline the relevance of cysteine-tagging techniques for MS-based proteomics.     

跨媒体彩色复杂影像再现品质效益评估模式-「空间滤波器」及「CIEDE2000色差公式」的结合应用

在现行跨媒体复制系统中,若应用CIELAB于复杂彩色影像的评估,则因其未考量影像中各色点与周遭色彩之间相互影响的因素,将导致转换后的影像色彩外貌,无法与人眼的感觉相吻合.所以,此研究的目的乃一希望植基于人眼视觉机制,推导出得以精确应用于跨媒体复杂影像与其复制品之间的色差评估模式.以近年来新推荐之CIEDE2000色差公式,结合"具人眼机制的空间滤波器技术",进行"SCIELAB型的色差模式"推导.另外,亦将进行观测者心理物理配对实验.再将配对实验的结果与前述之色差评估模式者相比较,以期建立出最佳化的"跨媒体彩色复杂影像的色差评估模式".     

Power Efficient SDR Implementation of IEEE 802.11a/p Physical Layer

Software defined physical layer modems can be considered the new trend in the field of communications. Differently from dedicated hardware, software can be easily modified to implement a large variety of standards on the same platform. The use of software can significantly reduce development costs, but generally comes at the price of an increase in silicon area and power consumption. For different reasons, this price is something that is not always convenient or even possible to pay, as in the case of low-cost ICs implementing a single waveform, or even multi-mode modems embedding legacy IPs already available in hardware. In particular, power consumption overhead can be prohibitive for mobile terminals or in general for battery-powered devices. The very first challenge for a computing fabric to be competitive is to find and implement the right trade-off between flexibility and performance. This was the guideline for the design of the Block Processing Engine (BPE), a template architecture conceived for power-efficient baseband processing. The BPE core feature is a mixed-grain instruction set balancing general-purpose fine-grain instructions with more specific coarse-grain instructions wrapping custom hardware modules. To further limit the power consumption, the BPE also implements instruction-pipelining, variable-size SIMD and multi-task support. To prove the efficiency of such an approach, a dual-mode IEEE 802.11a/p receiver has been implemented.     

Moment-Based Angular Difference Estimation Between Two Tomographic Projections in 2D and 3D

This paper introduces a new method for estimating the angular difference between two tomographic projections belonging to a set of projections taken at unknown directions in 2D and 3D. Our method relies on the projection neighbor selection in projection moment space, the calculation of the angular differences between these neighboring projections using moment properties and a projection moment neighborhood graph. The accuracy and the robustness of our method are shown on a test database including fifty 2D and 3D gray-level images at different resolutions and with different levels of noise.     

Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas

The objective of this work was to study the effects of washing and purification steps on qualitative and quantitative analysis of fecal stanols in the oyster Crassostrea gigas using either single or a combination of lipid purification steps on silica gel or aminopropyl bonded silica gel (NH₂) or a washing step. Among the three analytical pathways compared, the two including water extraction or NH₂ purification did not lead to higher recoveries and decreased repeatabilities of extractions compared to the single purification on silica gel. This latter led to similar recoveries (ca. 80 %) and repeatabilities (ca. 10 %) for both spiked standards (coprostanol and sitostanol). This analytical pathway has been applied to oysters collected in a harvesting area in Brittany (France) where fecal contaminations are important and allowed to quantify eight stanols in oysters. The relative proportions of fecal stanols of these oysters were combined with principal component analysis in order to investigate the usefulness of their stanol fingerprints to record a fecal contamination and to distinguish its source between human, porcine and bovine contaminations. Oysters non-fecally contaminated by Escherichia coli did not present specific stanol fingerprints while oysters fecally contaminated had a bovine fingerprint, suggesting a contamination of these samples by bovine sources. As a consequence, the method developed here allows the use of stanol fingerprints of oysters as a microbial source tracking tool that can be applied to shellfish harvesting areas subjected to fecal contaminations in order to identify the different sources of contamination and improve watershed management.     

Potential Use of Ascorbic Acid–Based Surfactants as Skin Penetration Enhancers

ABSTRACT

6-O-Ascorbic acid alkanoates (ASCn) are amphiphilic molecules having physical-chemical properties that depend on the alkyl chain length. The derivatives of low molecular weight (n < 11) have enough aqueous solubility to produce self-assemblies at room temperature (≈25°C), while those with longer alkyl chains possess a critical micellar temperature (CMT) higher than 30°C. At higher temperatures (T° > CMT), ASCn aqueous suspensions turn into either micellar solutions or gel phases, depending on the length of the hydrophobic chain. On cooling, coagels are produced, which possess a lamellar structure that exhibit sharp X-ray diffraction patterns and optical birefringence. The semisolid consistency of such coagels is an interesting property to formulate dermatological pharmaceutical dosage forms able to solubilize and stabilize different drugs. The objective of the present study was the evaluation of the enhancing permeation effect of ASCn with different chain lengths and to correlate permeability changes with histological effects. With this purpose, ASCn coagels containing anthralin (antipsoriasic drug) or fluorescein isothiocyanate (FITC, hydrophobic fluorescent marker) were assayed on rat skin (ex vivo) and mice skin (in vivo), respectively. Also, histological studies were performed aimed at detecting some possible side effects of ASCn. No inflammatory cellular response was observed in the skin when ASCn coagels were applied, suggesting non-irritating properties. Light microscopy indicated slight disruption and fragmentation of stratum corneum. The penetration of ASCn through rat skin epidermis was very fast and quantitatively significant. The permeation of anthralin was significantly increased when the drug was vehiculized in ASCn coagels, compared to other pharmaceutical systems. The results indicated that ASC12 seems to have the highest enhancing effect on FITC permeation. ASC12 appears to be the compound that possesses the highest capacity to enhance the penetration of the drugs. Furthermore, it has the highest permeation of the serie.     

Composite hydrogeological investigation and characterisation methods applied at a nuclear power plant site in Taiwan

Drawing from the lessons learnt from the Fukushima nuclear disaster in Japan, the assessment of subsurface environmental hazard around nuclear power plants has emerged as a critical task. Consequently, aiming to better understand the possible environmental impact of radiation leaks into the groundwater, a prescreening programme was initiated in 2011 by the Taiwanese government. As part of this programme, this study conducted various borehole prospecting techniques to identify in situ hydrogeological characteristics at Chin‐Shan Nuclear Power Plant. Borehole electrical log, sonic log and temperature/conductivity log were conducted to explain the regional lithologic conditions and permeability of the formation. In conjunction with this, the interwell tracer and pumping test was carried out to simultaneously determine the hydraulic parameters. In our opinion, the implementation of such in situ end‐to‐end investigations is essential in interpreting in situ fluid and solute transport dynamics prior to programming any numerical scheme for early warning, vulnerability assessment and regular monitoring of a nuclear power plant site.     

Evaluation of ascorbic acid and sugar degradation products during fruit dessert processing under conventional or ohmic heating treatment

The effects of conventional heating (C) or ohmic heating (O) treatment on the degradation of sugar and ascorbic acid (AA) were evaluated in chunky fruit desserts prepared with apple puree (Golden Delicious var.) and chunky peach (Panavi var.) pieces. Concentrations of 5-HMF, furfural (F), 3-hydroxy-2-pyrone (3H2P) and 2-furoic acid (FA) were determined to assess the importance of oxidative and/or thermal reactions during processing. Depending on the thermal processing, 5-HMF and F levels in samples ranged from not detected to 3260 and from not detected to 570 μg/100 g FW respectively. Production of 3H2P and FA ranged from 480 to 2670 and from 84 to 420 μg/100 g FW respectively. Results showed that the impact of final heating treatment on the formation of AA degradation products (3H2P and FA) was minor, if any. Thus, AA degradation during apple products processing mainly depended on oxidative reactions pathway. Likewise, ohmic heating processing had very little effect on the formation of 5-HMF and F, in opposite to conventional heating.     

Electricity rates for the zero marginal cost grid

The electricity industry is rapidly changing: costs are increasingly dominated by capital and technology is turning loads into resources. This is similar to the early days of the Internet. Building on rate-structures used in the communications industry, utilities of the future should offer customers a portfolio of service contract options that provide a signal to the utility regarding the type and amount of infrastructure that should be deployed.     

Nanoconfined mixed Li and Mg borohydrides as materials for solid state hydrogen storage

Several mixtures of LiBH₄ and Mg(BH₄)₂ borohydrides in different stoichiometric ratios (1:0, 2:1, 1:1, 1:2, 0:1), prepared by high energy ball milling, have been investigated with X-ray powder diffraction and thermal programmed desorption (TPD) volumetric analysis to test the dehydrogenation kinetics in correlation with the physical mixture composition. Afterwards mixed and unmixed borohydrides were dispersed on high specific surface area ball milled graphite by means of the solvent infiltration technique. BET and statistical thickness methods were used to characterize the support surface properties, and SEM micrographs gave a better understanding of the preparation techniques. It has been observed by TPD volumetric measurements that the confinement of the reactive borohydrides on the nanoporous supports leads to a lower dehydrogenation temperature compared to unsupported borohydrides. Moreover, a further decrease of the dehydrogenation temperature has been observed by increasing the specific surface area of the support and the pores volume and by using the prepared mixtures instead of pure materials. The dehydrogenation process seems to be favoured by the heterogeneous nucleation on the graphite surface of decomposition products or intermediate phases from melted liquid borohydrides.