Biosensor based on cellobiose dehydrogenase for detection of catecholamines

A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs Ag|AgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A.M(-1) x cm(-2) (approximately 1150 nA/microM) for noradrenaline, with a coverage of less than 2.5 microg of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear range, and amplification factor can be modulated by the steady-state concentration of cellobiose in the flow buffer. The response of the sensor decreases only 2% when run continuously for 4 h in the flow injection mode. The response peak maximum is obtained within 6 s at a flow rate of 0.5 mL/min, representing the time of the entire sample segment to pass the electrode. CDH enzymes from Phanerochaete chrysosporium and Sclerotium rolfsii were investigated, providing different characteristics of the sensor, with sensors made with CDH from P. chrysosporium being the better ones.     

Polariscope for simultaneous measurement of the principal axis and the phase retardation by use of two phase-locked extractions

A novel polariscope with electro-optic modulation that is capable of simultaneous measurement of the principal axis and the phase retardation of an optical linear birefringent medium by means of two phase-locked extractions is described. A phase compensator is used to suppress the transmission phase-retardation effect of the beam splitter, thereby enhancing the precision of the measuring performance. The validity of the proposed design is demonstrated by measurement of the principal axis and phase retardation of a quarter-wave plate sample. There are absolute errors of 0.25 degrees on average and 0.58 degrees at maximum in the principal-axis measurement and of 0.75 degrees (0.83%) on average and 3.11 degrees at maximum in the phase-retardation measurement. Meanwhile, the retardation error lies within a 5% uncertainty range of a commercial wave plate. The root-mean-square resolutions for the principal-axis angle and phase-retardation measurements are 0.042 degrees and 0.081 degrees, respectively. Finally, the dynamic ranges of the principal-axis angle measurement and the phase-retardation measurement extend as far as 180 degrees.     

Optical implementation of the hopfield neural network with matrix gratings

We propose a new method for the optical implementation of the Hopfield neural network with a universal tool. The tool is a matrix grating constituted with a group of element gratings. The algorithms for designing a matrix grating are proposed, and a matrix grating is created to execute recognition experiments by use of the Hopfield neural network. The experimental results demonstrate that the proposed method performs well. The stability of the light efficiencies of different optical components used in optical networks is also considered.     

A simple program which processes liquid scintillation counting data from samples containing both 125I and 3H under conditions of varying quench

A human study of the effects of topical nystatin (Mycostatin) therapy of oral candidiasis showed that effects of treatment were limited to the time in which the drug was used. Two weeks of therapy resulted in significant reduction in number of organisms and marked improvement in signs and symptoms of candidiasis. The condition recurred rapidly following cessation of treatment. No change in specific anticandida antibody in saliva or in adherence of Candida albicans to mucosal epithelium (in vitro) was seen with treatment.     

Statistical intensity of amplitude scintillations on Earth-space X-band link

Amplitude fluctuation measurements made on a 30° of elevation earth-space link at 11.6 GHz using the Orbital Test Satellite transmissions are described. Results on the intensity and cumulative distribution of the scintillations between 0.01 Hz and 14 Hz are presented and show that for percentages of time less than 20% the peak amplitude deviations from the mean exceed the values derived using a log-normal distribution and the average r.m.s. value. For greater time percentages the agreement with a log-normal distribution is good.     

Accuracy of self-reported data for estimating crash severity

Estimated traveling speed and speed limit have typically been used in population-based surveillance data to estimate crash severity. The accuracy of these measures in predicting crash severity is unknown. The Partners for Child Passenger Safety (PCPS) surveillance system offers a unique opportunity to compare these measures, as well as a novel measure of crash severity, “self-report” delta-V, to the accepted measure of delta-V estimated during detailed crash-investigations in 118 crashes. This “self-report” delta-V was computed from the estimated traveling speeds and direction of impact obtained from telephone interviews with drivers. These “self-reported” delta-V estimates are modestly associated with crash-investigation delta-V estimates, with the degree of association a function of the direction of impact: when the respondent was struck from the rear, the degree of association is strong; frontal, side, and single-vehicle crashes yield weaker associations. This “self-reported” delta-V measure, however, is a substantial improvement over use of estimated traveling speed or speed limit only.     

A framework for efficient regression tests on database applications

Regression testing is an important software maintenance activity to ensure the integrity of a software after modification. However, most methods and tools developed for software testing today do not work well for database applications; these tools only work well if applications are stateless or tests can be designed in such a way that they do not alter the state. To execute tests for database applications efficiently, the challenge is to control the state of the database during testing and to order the test runs such that expensive database reset operations that bring the database into the right state need to be executed as seldom as possible. This work devises a regression testing framework for database applications so that test runs can be executed in parallel. The goal is to achieve linear speed-up and/or exploit the available resources as well as possible. This problem is challenging because parallel testing needs to consider both load balancing and controlling the state of the database. Experimental results show that test run execution can achieve linear speed-up by using the proposed framework.     

A Petri-Net Approach to Modular Supervision With Conflict Resolution for Semiconductor Manufacturing Systems

In a semiconductor manufacturing system, particular human operations may violate desired requirements and lead to destructive failure. For such human-in-the-loop systems, this paper proposes a supervisory framework which guarantees that manual operations meet required specifications so as to prevent human errors in operation using Petri nets. Moreover, a modular technique with an intersection mechanism is proposed in order to cope with the state-space explosion problem of large-scale systems. A rapid thermal process in semiconductor manufacturing systems is provided to show the practicability of the proposed approach. Note to Practitioners - This work was motivated by the requirement of remote monitoring and supervision of semiconductor manufacturing systems. In such human-in-the-loop and large-scale systems, certain human operations may violate desired safety requirements and result in catastrophic failure. Moreover, the overall complexity of most existing Petri net modeling and analysis approaches significantly increases with the size of the considered systems. This paper suggests a modular supervisory scheme for modeling and synthesis of supervisory agents using Petri nets. The proposed method contributes a promising tool for preventing abnormal operations from being carried out to semiconductor manufacturing systems which, if appropriately modified, may be also applied to other types of discrete event systems.     

Chemical-Enhanced Washing for Remediation of Soils Contaminated with Marine Diesel Fuel in the Presence/Absence of Pb

The effects of the operating conditions, the initial concentrations of marine diesel fuel (MDF) and the coexisting Pb in the soil, and the ethylene diamine tetra acetic salt (EDTA) in solution on MDF removal by sodium dodecyl sulfate (SDS) washing were extensively investigated with the aim of optimizing the process parameters and determining the MDF removal efficiency by SDS under different contamination conditions. The experimental results from batch tests indicated that the majority of MDF was removed by SDS in the first 2?h, and its optimal pH was nearly neutral. Increasing the SDS concentration linearly increased the MDF removal efficiency. At a given SDS concentration, the removal efficiency was dependent on the existing forms of MDF in soils, and the free phase of MDF was found to be more easily removed than the adsorbed phase. MDF removal by SDS was significantly reduced by the coexisting Pb in soils, which likely forms a complexation with SDS and thereby enhances the partitioning of MDF in the soil by the re-adsorption of released MDF onto the hydrophobic tails of the adsorbed SDS. EDTA alone, or with SDS, could remove MDF, but the remaining MDF in the contaminated soil after EDTA washing became more difficult to be removed by SDS. Therefore, the EDTA washing followed by SDS washing is not recommended for MDF removal.     

A Wide Tuning Range Gm –C Continuous-Time Analog Filter

A CMOS operational transconductance amplifier (OTA) for low-power and wide tuning range filter application is proposed in this paper. The OTA can work from the weak inversion region to the strong inversion region to maximize the transconductance tuning range. The transconductance can be tuned by changing its bias current. A fifth-order Elliptic low-pass filter implemented with the OTAs was integrated by TSMC 0.18-mum CMOS process. The filter can operate with the cutoff frequency of 250 Hz to 1 MHz. The wide tuning range filter would be suitable for multi-mode applications, especially under the consideration of saving chip areas. The third-order inter-modulation (IM3) of -40 dB was measured over the tuning range with two tone input signals. The power consumption is 0.8 mW at 1-MHz cutoff frequency and 1.8-V supply voltage with the active area less than 0.3 mm²