Complex Mechanisms of Antimony Genotoxicity in Budding Yeast Involves Replication and Topoisomerase I-Associated DNA Lesions,Telomere Dysfunction and Inhibition of DNA Repair

Antimony is a toxic metalloid with poorly understood mechanisms of toxicity and uncertain carcinogenic properties. By using a combination of genetic, biochemical and DNA damage assays, we investigated the genotoxic potential of trivalent antimony in the model organism Saccharomyces cerevisiae. We found that low doses of Sb(III) generate various forms of DNA damage including replication and topoisomerase I-dependent DNA lesions as well as oxidative stress and replication-independent DNA breaks accompanied by activation of DNA damage checkpoints and formation of recombination repair centers. At higher concentrations of Sb(III), moderately increased oxidative DNA damage is also observed. Consistently, base excision, DNA damage tolerance and homologous recombination repair pathways contribute to Sb(III) tolerance. In addition, we provided evidence suggesting that Sb(III) causes telomere dysfunction. Finally, we showed that Sb(III) negatively effects repair of double-strand DNA breaks and distorts actin and microtubule cytoskeleton. In sum, our results indicate that Sb(III) exhibits a significant genotoxic activity in budding yeast.     

Differences in the mineral composition of sound and disordered apple fruits and of sound and pitted tissue

Separation and analyses of sound Cox's Orange Pippin apples and those with disorders, after storage for 18 weeks at 3.3°C, revealed that sound apples had the lowest, apples rotted by Gloeosporium spp slightly higher, apples with bitter pit higher, and apples affected by breakdown even higher proportions of calcium in the residue after extraction with distilled water, although total concentrations of calcium in these samples were in the reverse order. Lower proportions of calcium, magnesium and potassium were extracted by 75% ethanol from fruit with breakdown than from sound apples, and similarly lower proportions of magnesium, and magnesium and potassium were extracted by water from apples with bitter pit and breakdown, respectively. Proportions of phosphorus extracted by either solvent were not obviously related to disorders. The high concentration of magnesium in pitted tissue, in relation to that in adjacent sound tissue, was evenly divided between the fractions soluble and insoluble in water, but that of calcium was mainly in the insoluble fraction.     

Hybrid DPWM implementation using coarse and fine programmable ADLL

In the paper we propose a novel architecture and implementation of 11-bit Digital Pulse Width Modulator (DPWM) circuit based on previously known building blocks. Linearized Class-AD Double-sided (LADD) algorithm has been used to calculate the DPWM signals of the 11-bit resolution hybrid DPWM for a Class-AD digital audio amplifier. Noise-shaping process is used to support high fidelity with practical values of time resolution. The proposed DPWM circuit is composed of 8-bit counter and Analog Delay Locked Loop (ADLL) using 4-bit tapped delay line. A dual ADLL employing coarse and fine programmable delay element is used to adjust the delay time of delay line and lock it to required time. The coarse- as well as fine-delay lines are implemented as a cascade of variable-delay elements based on shunt capacitor delay element or single-ended Schmitt trigger. The proposed 11-bit DPWM circuit, at a switching frequency of 352.8 kHz and clock generator frequency of 90.3 MHz allows us to attain SNR of 120 dB and THD of the output signal less than 0.1% within the audio baseband and modulation index M=0.95. Basic verification of circuit manufacturability and simulation results (Monte Carlo analysis) for real CMOS process are presented.     

A multiobjective design of a patient and anaesthetist-friendly neuromuscular blockade controller

During surgeries (especially in long ones), patients are subject to a substantial amount of drug dosage necessary to achieve the required neuromuscular blockade level. This paper aims at the development of a fuzzy controller that satisfies two important goals: 1) an optimization of the amount of drug (atracurium) required to induce an adequate level of relaxation and 2) a concomitant ability to explain the undertaken control decision at the level of natural language. For instance, statements of the form "Since the difference between the target and the current blockade level is near zero, a small quantity of drug infusion is currently being applied", where "near zero" and "small" are linguistic terms that are represented as fuzzy sets. In this sense, we can regard this controller as a construct that is human friendly and highly interpretable (transparent). To address the two objectives outlined above, we consider the use of a multiobjective evolutionary optimization. How the quality of the control action and the controller interpretability are formalized and captured in this optimization framework is presented. The effectiveness of the approach is demonstrated through a comprehensive suite of experiments involving 100 simulated patients (used for training) and 500 patients (forming the test set), validating the approach for application in the operating theater.     

Scheduling Algorithms and Throughput Maximization for the Downlink of Packet-Data Cellular Systems with Multiple Antennas at the Base Station

The capacity-achieving coding scheme for the multiple-input multiple-output (MIMO) broadcast channel is dirty-paper coding. With this type of transmission scheme the optimal number of active users that receive data and the optimal power allocation strategy are highly dependent on the structure of the channel matrix and on the total transmit power available. In the context of packet-data access with adaptive transmission where mobile users are equipped with a single receive antenna and the base station has multiple transmit antennas, we study the optimal number of active users and the optimal power allocation. In the particular case of two transmit antennas, we prove that the optimal number of active users can be a non-monotonic function of the total transmit power. Thus not only the number of users that should optimally be served simultaneously depends on the user channel vectors but also on the power available at the base station transmitter. The expected complexity of optimal scheduling algorithms is thus very high. Yet we then prove that at most as many users as the number of transmit antennas are allocated a large amount of power asymptotically in the high-power region in order to achieve the sum-capacity. Simulations confirm that constraining the number of active users to be no more than the number of transmit antennas incurs only a marginal loss in spectral efficiency. Based on these observations, we propose low-complexity scheduling algorithms with sub-optimal transmission schemes that can approach the sum-capacity of the MIMO broadcast channel by taking advantage of multiuser diversity. The suitability of known antenna selection algorithms is also demonstrated. We consider the cases of complete and partial channel knowledge at the transmitter. We provide simulation results to illustrate our conclusions.     

Microvaristors in thick-film and LTCC circuits

ZnO-based varistors protect electronic circuits against overvoltage. High temperature from the range of 1150-1300 °C is required for proper sintering of such material. Varistor inks with lower firing temperature are needed for application in thick-film and LTCC technology. ZnO-based thick-film composition was prepared and varistors were fabricated on alumina and LTCC substrate. Different topologies (capacitor-like or planar), electrode metallurgies (PdAg, Au or Pt-based) and firing profiles (850 °C or 950 °C) were used. Samples microstructure was investigated. Varistor I-V characteristics, long-term stability and durability to high voltage pulses were examined. Satisfactory results were achieved, because nonlinearity coefficient α up to 23 was obtained for capacitor-like varistors with Pt terminations on LTCC substrates, long-term thermally aged (150 h at 250 °C) varistors had slightly smaller nonlinearity coefficient and characteristic voltage, V_1 mA and components subjected to series of high voltage pulse (1000 pulses with 10 mA amplitude and 5 ms duration each) exhibited almost the same electrical parameters.     

An application of scanning electron microscopy combined with roentgen microanalysis (SEM-EDS) in canine urolithiasis

Urolithiasis is a common diagnostic and therapeutic problem in small-animal veterinary practice. The traditional diagnostic approach usually consists of clinical, radiological and ultrasonographic examination of the patient. The main diagnostic material is still urine sediment, ignoring the fact that presence of crystalluria is not always of pathological significance. In order to establish the most effective therapeutic and preventative strategies, especially in the case of multicomponent stone, it is crucial to define the exact elemental composition of the given stone including crystallization nidus chemical contents. In the course of the research, the usefulness of scanning electron microscopy combined with X-ray-dispersive spectrometry in analysis of canine mixed and compound stones was investigated. The obtained results indicated that the tested method allows one to trace the dynamics of the crystallization process, including crystallization nucleus detection, and concurrently and quantitatively assess the elemental composition of the given urinary concrement. Moreover, the conducted research showed epidemiological data of urolithiasis occurrence in a population of dogs coming from the southern part of Poland.     

A comparative study of hygroscopic moisture content,electrical conductivity and ion chromatography for salt assessment in plasters of historical buildings

It has long been known that highly soluble salts or hydrate-forming salts can damage porous building material. There is a real challenge to provide salt assessment in compliance with good monuments diagnosis and restoration practices. The salts present in the masonry can be analysed qualitatively and quantitatively by means of several techniques. Nowadays, the standard analytical equipment as ion chromatography (IC) found in several laboratories is a reliable method for analysing a large variety of anions and cations in solutions (or building materials water extracts), in order to meet international standards and regulations. However, preliminary study and diagnosis of soluble salt present in monuments do not necessarily require such a precise (SD < 5%) and sensitive (ppm or ppb range) analyses. Some simple-to-use techniques, such as hygroscopic moisture content (HMC) and electrical conductivity (EC) of extracted salt solutions, are widely in use to provide indications on the presence of salts.This paper presents a comparison of salt assessment obtained by IC, HMC and EC methods, and provides the domain of their application to specific questions linked to the damaged building diagnosis and preservation.The comparison of the results obtained by IC, EC and HMC analyses highlights the limits of the EC method in providing quantitative information on soluble salt content. The EC technique seems to require some cautions when used for materials containing other high soluble phases such as portlandite. On the other hand, the HMC technique seems to be very useful in the field of historical masonry investigation since it can provide reliable semi-quantitative distribution of hygroscopic salts.