Heat resistance of Listeria monocytogenes

The heat resistance data on Listeria monocytogenes in culture media and foods are summarized. Most heat resistance data for foods have been obtained in dairy, meat, poultry, and egg products. Limited data have been published on seafood, fruits, and vegetables. The methodologies employed have evolved over time; hence data from earlier experiments are not directly comparable to more recent studies. Many factors influence the heat resistance of L. monocytogenes. Variation exists among different strains in their ability to withstand heat treatment. In addition, heat resistance is influenced by age of the culture, growth conditions, recovery media, and characteristics of foods such as salt content, a(w), acidity, and the presence of other inhibitors. Listeriae are more heat resistant than most other nonspore-forming foodborne pathogens, and thus, processing recommendations based on data from experiments with Salmonella spp. or pathogenic Escherichia coli may not be sufficient to eliminate similar numbers of L. monocytogenes. The data provided in this review may prove useful for food processors in determining appropriate times and temperatures for producing foods free of vegetative pathogens.     

Microstructure changes in Sn-3.5Ag solder alloy during creep

Experimental data on behavior of the Sn-3.5Ag solder alloy microstructure during tensile creep deformation and subsequent failure is described. Depending upon applied stress, the nucleation and further development of grain boundary defects that start at an earlier stage in the deformation process was revealed. A discussion is presented on possible micromechanisms of the crack formation process.     

Quantitative trait loci and metabolic pathways

The interpretation of quantitative trait locus (QTL) studies is limited by the lack of information on metabolic pathways leading to most economic traits. Inferences about the roles of the underlying genes with a pathway or the nature of their interaction with other loci are generally not possible. An exception is resistance to the corn earworm Helicoverpa zea (Boddie) in maize (Zea mays L.) because of maysin, a C-glycosyl flavone synthesized in silks via a branch of the well characterized flavonoid pathway. Our results using flavone synthesis as a model QTL system indicate: (i) the importance of regulatory loci as QTLs, (ii) the importance of interconnecting biochemical pathways on product levels, (iii) evidence for "channeling" of intermediates, allowing independent synthesis of related compounds, (iv) the utility of QTL analysis in clarifying the role of specific genes in a biochemical pathway, and (v) identification of a previously unknown locus on chromosome 9S affecting flavone level. A greater understanding of the genetic basis of maysin synthesis and associated corn earworm resistance should lead to improved breeding strategies. More broadly, the insights gained in relating a defined genetic and biochemical pathway affecting a quantitative trait should enhance interpretation of the biological basis of variation for other quantitative traits.     

Stabilisation of invertases by treatment with cross-linking reagents

Commercial baker's yeast invertase concentrate, used in the manufacture of a variety of confectionary products, was treated with various chemical cross-linking agents in an attempt to improve its thermal stability to that of the more useful invertase from Candida utilis, which has been shown previously to have high thermal stability. Only glutaraldehyde was successful but at the expense of a loss of enzyme activity and a three-fold increase in K_m. Dimethylsuberimidate stabilised commercial invertase only slightly and the other reagents tried, either had no effect upon stability or caused a destabilisation of the enzyme. C. utilis invertase can be stabilised even further against thermal denaturation by dimethylsuberimidate. A similar stabilisation was found when this enzyme was heated in the presence of ≥ 10 mM-CaCl₂.     

Active damping control of a high-power PWM current-source rectifier for line-current THD reduction

The use of active damping to reduce the total harmonic distortion (THD) of the line current for medium-voltage (2.3-7.2 kV) high-power pulsewidth-modulation (PWM) current-source rectifiers is investigated. The rectifier requires an LC filter connected at its input terminals, which constitutes an LC resonant mode. The lightly damped LC filter is prone to series and parallel resonances when tuned to a system harmonic either from the utility or from the PWM rectifier. These issues are traditionally addressed at the design stage by properly choosing the filter resonant frequency. This approach may result in a limited performance since the LC resonant frequency is a function of the power system impedance, which usually varies with power system operating conditions. In this paper, an active damping control method is proposed for the reduction in line current THD of high-power current-source rectifiers operating at a switching frequency of only 540 Hz. Two types of LC resonances are investigated: the parallel resonance excited by harmonic currents drawn by the rectifier and the series resonance caused by harmonic pollution in the source voltage. It is demonstrated through simulation and experiments that the proposed active damping control can effectively reduce the line-current THD caused by both parallel and series resonances.     

Chemical and ultrastructural analysis of corn cuticular lipids and their effect on feeding by fall armyworm larvae

The cuticular lipid composition of lower and upper leaves of five genotypes of field-grown corn,Zea mays L., was determined by combined gas chromatography-mass spectrometry. Surface lipids of the upper leaves had a higher proportion ofn-alkanes (45–52%) than the lower leaves, while the lower leaves had higher percentages of fatty alcohols (12–18%) than the upper leaves. Scanning electron microscopy showed that the upper leaves of two corn genotypes, MpSWCB-4 and Cacahuacintle X's, had a smooth amorphous appearance, while the lower leaves had a dense array of wax crystals.Spodoptera frugiperda (J.E. Smith) larvae weighed more and developed more rapidly when they were reared on diet containing corn foliage from which the cuticular lipids had been removed than when they were fed untreated foliage. However, growth was not inhibited when larvae were fed diet containing the cuticular lipid extracts or individual cuticular lipid components.