Ein Beitrag zur Biosynthese amylosereicher Erbsenstärken

Contribution to the Biosynthesis of High-amylose Pea Starches Biosynthesis of starch is a complex process but few details are still unknown. This paper describes an investigation upon pea starch biosynthesis. Peas as a source of starch offer two qualities, a pea starch with 40% amylose content (smooth pea starch) and a pea starch with more than 60% amylose content (wrinkled pea starch). Samples for analysis were taken during growth. According to the quantitative determination of starch and amylose the accuracy of amylose detection was tested. Furthermore a qualitative analysis was carried out by molecular weight estimation of the starch. Both kinds of peas showed a high amylose synthesis during the last few days of growth, whereas amylopectin synthesis was highly in earlier stages. Wrinkled peas have shown only little synthesis activity of amylopectin during maturity. Therefore the amylose content of these peas was higher, instead of equal amylose synthesis quotas of both smooth and wrinkled peas.     

SEM and UV-microscopic investigation of glue lines in Parallam® PSL

? PSL were investigated using scanning electron- and UV-microscopy. In the SEM it was found that resin penetrates predominantly through cut open tracheids and rays. Bordered pits block the flow of resin from cell to cell whereas simple pits are only a minor obstacle. UV-absorbance spectra of cells from the glue line area indicated the presence of phenol-formaldehyde resin in the wood cell wall. Compared to the pattern of absorbance of pure cell wall substance, spectra of cells embedded in the glue showed significant differences which were attributed to the penetration of phenol-formaldehyde resin during the process of gluing.

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? PSL wurden mit dem Rasterelektronenmikroskop und dem UV-Mikroskop untersucht. Im REM zeigte sich, da? Leim vor allem in angeschnittene Tracheiden und in Holzstrahlen eindringt. Ein Flie?en durch Hoftüpfel konnte nicht nachgewiesen werden, wohingegen einfache Tüpfel ein geringeres Hindernis darstellen. UV-Absorptionsspektren von Holzzellen aus dem Leimfugenbereich deuteten auf das Vorhandensein von Phenol-Formaldehydharz in der Zellwand hin. Die Spektren dieser Zellen wiesen im Vergleich zum Absorptionsverhalten der reinen Zellwandsubstanz deutliche Unterschiede auf, die auf das Eindringen von Phenol-Formaldehydharz w?hrend des Verleimvorganges schlie?en lie?en.

     

On alternation

Summary Every alternating t(n)-time bounded multitape Turing machine can be simulated by an alternating t(n)-time bounded 1-tape Turing machine. Every nondeterministic t(n)-time bounded 1-tape Turing machine can be simulated by an alternating (n + (t(n)) ^1/2)-timebounded 1-tape Turing machine. For wellbehaved functions t(n) every nondeterministic t(n)-time bounded 1-tape Turing machine can be simulated by a deterministic ((nlogn)^1/2 + (t(n))^1/2)-tape bounded off-line Turing machine. These results improve or extend results by Chandra-Stockmeyer, Lipton-Tarjan and Paterson. A preliminary version of this paper was presented at the 19th IEEE-FOCS     

Polycyclic aromatic hydrocarbons (PAH) in various types of tea

For the analysis of the 16 PAH (EFSA-PAH), which are classified as priority for different food groups by the Scientific Committee on Food (SCF) and the Joint FAO/WHO Experts Committee on Food Additives (JECFA) in tea, a sensitive analytical Fast-GC/HRMS method was used. The sample preparation included accelerated solvent extraction (ASE) and the highly automated clean up steps, gel permeation chromatography and solid phase extraction. The analytical parameters, limit of detection (0.01–0.02 μg/kg) and limit of quantification (0.03–0.06 μg/kg), were determined. The repeatability (RSD, n = 3) of different PAH in fruit tea ranged from 0.1 to 11%. It was observed that the total contents of the 16 PAH in tea samples ranged from 14 to 2,662 μg/kg. The analysed tea samples showed an increasing presence of PAH in the following order: herbal and fruit tea (n = 7) < black tea (n = 11) < green tea (n = 11) < white tea (n = 3) < mate-tea (n = 8). The correlation coefficient (R) between BaP and the sum of the 16 EFSA-PAH was established considering the contamination amount in all the 40 tea samples analysed.     

Diamine oxidase supplementation improves symptoms in patients with histamine intolerance

Food Science and Biotechnology - Histamine intolerance (HIT) is thought to be caused by a disproportionate amount of histamine in the body. The enzyme diamine oxidase (DAO) is considered for the...     

Chemical composition,nutritive value and voluntary intake of tropical tree foliage and cocoyam in pigs

The composition of the leaves of cocoyam (Xanthosoma sagittifolium) and of two trees (Trichanthera gigantea and mulberry, Morus alba), their nutritive value in pigs and voluntary intake by pigs were determined. The average protein content ranged from 170 to 240 g kg^?1 dry matter (DM) and that of neutral detergent fibres from 218 to 398 g kg^?1 DM. The leaves are interesting sources of calcium (up to 69 g kg^?1 DM), potassium, iron and manganese. The proteins are well balanced in essential amino acids, with lysine ranging from 43 to 57 g kg^?1 proteins. The apparent faecal digestibility was determined by difference in 35 kg pigs fed a diet containing 35% leaf meal. The digestibility coefficients of DM, N and energy were, respectively, 47–57, 33–36 and 51–53%. The digestible energy value ranged from 1.674 to 2.037 kcal kg^?1 DM. The voluntary intake of Trichanthera and Xanthosoma was measured in sows weighing 100 kg on average. The intake reached 3.4 kg fresh leaves day^?1 (0.51 kg DM) and 1.0–1.1 kg dry leaf meal/day. It is concluded that low energy density is the main limiting factor of tree foliage for pig nutrition but that they are good sources of minerals and well‐balanced proteins. Copyright © 2005 Society of Chemical Industry