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991.
A sensitive method based on ferrocene-streptavidin (Fc-Stv) conjugates for the simultaneously amplified electrochemical and surface plasmon optical detection of DNA target hybridization to peptide nucleic acid-modified gold surfaces is reported. The attachment of Fc-Stv to the biotinylated complementary target DNA not only amplified the surface plasmon resonance signal but also enhanced the electrochemical signal due to the many Fc markers per Stv. The ferrocene redox peak current increased with the increase of the target DNA concentration. Consequently, the amount of hybridized target DNA can be estimated by cyclic voltammetry and chronocoulometry. The detection limit of this DNA sensor is 10 pM (2 fmol, with signal to noise > 3). This sensor was also shown to have high selectivity (at the single-base mismatch level) and good reproducibility. 相似文献
992.
The influence of the metal film thickness (i.e., the chromium adhesion promoting film and the gold film) on the sensitivity of surface plasmon resonance (SPR) signals (i.e., resonance angle shift and reflectance change) towards the thickness variation of the nonabsorbing dielectric film is investigated. The sensitivity of reflectance change decreases when a thick chromium film or a thin gold film is employed. Its linear range becomes narrower as the thickness of the metal films increases. The sensitivity and linear range of the resonance angle shift are not affected by the thickness variation of the metal films. The phenomena were theoretically explained based on the attenuated total reflection (ATR) generated evanescent field at the prism/metal interface and the SPR-generated evanescent field at the metal/dielectric interface. 相似文献
993.
Apoorva G. Wagh Veer Chand Rakhecha Makus Strobl Wolfgang Treimer 《Journal of research of the National Institute of Standards and Technology》2005,110(3):231-235
Super Ultra-Small Angle Neutron Scattering (SUSANS) studies over wave vector transfers of 10–4 nm–1 to 10–3 nm–1 afford information on micrometer-size agglomerates in samples. Using a right-angled magnetic air prism, we have achieved a separation of ≈10 arcsec between ≈2 arcsec wide up- and down-spin peaks of 0.54 nm neutrons. The SUSANS instrument has thus been equipped with the polarized neutron option. The samples are placed in a uniform vertical field of 8.8 × 104 A/m (1.1 kOe). Several magnetic alloy ribbon samples broaden the up-spin neutron peak significantly over the ±1.3 × 10–3 nm–1 range, while leaving the down-spin peak essentially unaltered. Fourier transforms of these SUSANS spectra corrected for the instrument resolution, yield micrometer-range pair distribution functions for up- and down-spin neutrons as well as the nuclear and magnetic scattering length density distributions in the samples. 相似文献
994.
Wolfgang Paa Wolfgang Triebel Christian Eigenbrod Mikhail Larionov Adolf Giesen 《Microgravity science and technology》2005,17(3):71-74
We report about recent developments concerning the "Advanced Disk Laser" system, which is intended for use in combustion experiments
under microgravity conditions at the drop tower "Bremen". Since the system will be integrated into a modified drop capsule
it has to be a compact and efficient solid state laser which can withstand decelerations of up to 40 g. The existing laboratory
set up was redesigned to fit into the capsule. Therefore, the system layout was split in three platforms for optical components
(seed laser, regenerative amplifier and frequency conversion) and one additional platform for media supplies. Afterwards,
a special developed ultra stable structure was equipped with newly designed mechanical and electro-optical components, which
in part have already been tested in drop experiments. Access for alignments is possible via remote control of a number of
motorized mechanical mounts. The three optical platforms build a vibration-insulated, dust-free laser capsule of their own
inside the drop capsule and can be used both in the capsule and in a laboratory environment. The new laser system was already
optically adjusted and tested in the laboratory and reached a pulse energy of 20 mJ, as was expected. An extensive evaluation
period is going to follow and will be finished with a series of combustion experiments. 相似文献
995.
Graham AP Duesberg GS Seidel RV Liebau M Unger E Pamler W Kreupl F Hoenlein W 《Small (Weinheim an der Bergstrasse, Germany)》2005,1(4):382-390
Despite all prophecies of its end, silicon-based microelectronics still follows Moore's Law and continues to develop rapidly. However, the inherent physical limits will eventually be reached. Carbon nanotubes offer the potential for further miniaturization as long as it is possible to selectively deposit them with defined properties. 相似文献
996.
Pellegrino T Kudera S Liedl T Muñoz Javier A Manna L Parak WJ 《Small (Weinheim an der Bergstrasse, Germany)》2005,1(1):48-63
In this Review, we describe the synthesis of high-quality colloidal nanoparticles in organic solvents, the mechanisms by which they can be transferred into aqueous solution, and some of their applications in biology. In particular, we will place emphasis on the creation of multifunctional nanoparticles or nanoparticle assemblies. 相似文献
997.
Rümmeli MH Borowiak-Palen E Gemming T Pichler T Knupfer M Kalbác M Dunsch L Jost O Silva SR Pompe W Büchner B 《Nano letters》2005,5(7):1209-1215
In this letter, we show for the first time the use of metal oxides as catalysts in the synthesis of single-walled carbon nanotubes (SWCNTs) using laser ablation. Further, SWCNTs have been synthesized at low temperature (down to room temperature), where their nucleation cannot be explained via fullerene nucleation. The data point to a nucleation mechanism previously not identified, that places a stable oxidized ring as the root cause for the growth of SWCNTs. 相似文献
998.
In this paper we discuss the potentials of a new Bayesian inference tool, called the Gibbs sampler, for the analysis of the censored regression or Tobit model. Tobit models have a wide range of applications in empirical sciences, like econometrics and biometrics. The estimation results of the simple Tobit model will be compared to a hierarchical Tobit model, and the Gibbs sampling approach to the related classical algorithm of expectation-maximisation (EM). The underlying botanical example of this paper is concerned with the censoring mechanism in plant reproduction and proposes the Bayesian Tobit model for the growth relationship between the reproductive part and the rest of the plant. 相似文献
999.
Wolfgang K. Maser 《Advanced materials (Deerfield Beach, Fla.)》1994,6(1):87-87
1000.
Westerhausen René; Woerner Wolfgang; Kreuder Frank; Schweiger Elisabeth; Hugdahl Kenneth; Wittling Werner 《Canadian Metallurgical Quarterly》2006,20(3):272
The objective of the present study was to examine the role of the corpus callosum (CC) in dichotic listening. For this purpose, 40 right-handed healthy male participants were assessed with morphological and diffusion tensor imaging, which yielded macrostructural (midsagittal area) and microstructural (mean diffusion, fractional anisotropy) measures of the total CC and predefined subregions. Applying the standard consonant-vowel Bergen Dichotic Listening Test (including 3 different attentional instructions), the authors found dichotic listening performance to be substantially related to the integrity of the CC at both macro- and microstructural levels. Results indicate a dual role of the CC; it is relevant not only for the stimulus-driven (bottom-up) transfer of left-ear input to the left hemisphere but also for its attentional (top-down) modulation. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献