XC气田烃类渗漏的生态环境问题探讨

利用中子活化法对四川盆地XC气田的天然气进行分析，发现其中含有20多种金属元素。通过剖面和观察点的观察分析，发现XC气田烃类渗漏，尤其是天然气泄漏对生态环境造成了较大的负面影响：改变浅表生态环境，造成植物生长发育异常，农作牧减产；降低土壤pH值，增加金属元素的水溶态，从而增加植物对其的吸收消化；同时，植物还能从天然气中直接吸收消化一些金属元素。因此，天然气泄漏使农作物吸收消化过多的有毒有害金属，损害农产品品质，应当引起严重关注。     

Charge： Glowing in the Dark

Many areas of structure analysis have focused on finding the locations of the atoms. If, instead of the atoms, we knew the positions of the electrons we would in fact know much more, and in most respects knowing where the nuclei are is not important. There is a long history of measurements using precise electron diffraction or X-ray diffraction of the average charge density in materials, but extending beyond to local charge density is, as yet, an unexplored area.     

温州市龙湾段标准堤工程软土地基变形原位观测

温州市龙湾段标准堤地基土中普遍存在一层海积淤泥,其强度低,压缩性高,承载力低,不能满足标准堤填筑及水闸运行的要求。采用预压法进行地基处理,取得的地基沉降、水平位移、孔隙水压力等现场观测资料,总结分析了该段海积淤泥地基的变形规律和特点。并提供了有关的原型观测数据,其沉降曲线规律性较好。     

火成岩储层测井评价进展综述

十多年来,国内外火成岩储层测井评价已从交会图定性分析发展到用岩心分析资料建立裂缝性储层的测井解释模型，半定量、定量评价裂缝。成像测井和核磁测井的纵、横向分辨率高，经过地质刻度，可以较精细地识别岩性和裂缝，在解释火成岩方面有良好的效果。提高岩性识别准确率，识别复杂火成岩储层和孔隙流体，以及综合评价火成岩储层裂缝、饱和度等参数，仍然是火成岩储层测井评价所面临的难题。加强岩石物理基础实验，研究火成岩储层导电机理，系统研究火成岩储层测井评价理论，推广测井新技术在火成岩中的应用，建立统一的测井评价方法，将成为火成岩储层测井评价的发展趋势。     

基于Intranet/Internet的大坝安全监测信息查询系统

大坝安全监测信息的网上发布可以让人们了解各种实时、动态信息，文 中主要介绍了在Intranet/Internet下采用ASP(active server pages)结合VBScript，JavaS cript及ActiveX技术进行大坝安全监测信息查询系统开发的方法及步骤，并结合实际系统的 开发加以说明。     

Effect of alendronate treatment on the osteoclastogenic potential of bone marrow cells in mice

The expression of cytokeratins (CK), involucrin, vimentin, CD34, and alpha-smooth-muscle actin was studied in fetal and adult hair follicles. The first stage of the developing hair follicle is characterized by palisaded, elongated epithelial cells budding from the epidermal basal layer. These cells express CK5/6, CK14, CK17, CK19, and vimentin. During the following weeks of gestation, different structures in the developing hair follicle can be identified and characterized. The matrical cells display only CK19. The keratinocytes of the outer root sheath express CK5/6, CK14, CK17, CK19, and involucrin; those of the inner root sheath, CK4, CK18, and involucrin; those of the isthmus, the same profile as the ORS. In the infundibulum, the basal-layer keratinocytes express CK5/6, CK14, CK17, and CK19, whereas in the suprabasal layers CK1, CK4, CK10, CK14, and CK17 are seen. The adult hair follicle in anagen fails to express CK19 in the matrical cells and isthmus and both CK17 and CK19 in the infundibulum. These profiles of intermediate filaments and other markers appear to be potentially useful in categorizing neoplasms with apparent follicular differentiation.     

Purification of the cardiac sarcoplasmic reticulum membrane protein phospholamban from recombinant Escherichia coli

Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification.     

夯扩挤密水泥土桩用于高层建筑地基处理的尝试

水泥土桩已普遍用于多层住宅的地基处理，根据水泥土和夯扩挤密施工法的特点，在对夯扩挤密水泥土桩用于高层建筑地基处理进行研究、阐明其经济合理且安全可行的基础上，在高层住宅工程中进行了尝试，已取得令人满意的效果。