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61.
The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants. 相似文献
62.
ID Dukes S Sreenan MW Roe M Levisetti YP Zhou D Ostrega GI Bell M Pontoglio M Yaniv L Philipson KS Polonsky 《Canadian Metallurgical Quarterly》1998,273(38):24457-24464
Mutations in the hepatocyte nuclear factor-1alpha (HNF-1alpha) gene cause maturity onset diabetes of the young type 3, a form of type 2 diabetes mellitus. In mice lacking the HNF-1alpha gene, insulin secretion and intracellular calcium ([Ca2+]i) responses were impaired following stimulation with nutrient secretagogues such as glucose and glyceraldehyde but normal with non-nutrient stimuli such as potassium chloride. Patch clamp recordings revealed ATP-sensitive K+ currents (KATP) in beta-cells that were insensitive to suppression by glucose but normally sensitive to ATP. Exposure to mitochondrial substrates suppressed KATP, elevated [Ca2+]i, and corrected the insulin secretion defect. NAD(P)H responses to glucose were substantially reduced, and inhibitors of glycolytic NADH generation reproduced the mutant phenotype in normal islets. Flux of glucose through glycolysis in islets from mutant mice was reduced, as a result of which ATP generation in response to glucose was impaired. We conclude that hepatocyte nuclear factor-1alpha diabetes results from defective beta-cell glycolytic signaling, which is potentially correctable using substrates that bypass the defect. 相似文献
63.
Anomaly detection in web documents using crisp and fuzzy-based cosine clustering methodology 总被引:1,自引:0,他引:1
Cluster analysis is a primary tool for detecting anomalous behavior in real-world data such as web documents, medical records of patients or other personal data. Most existing methods for document clustering are based on the classical vector-space model, which represents each document by a fixed-size vector of weighted key terms often referred to as key phrases. Since vector representations of documents are frequently very sparse, inverted files are used to prevent a tremendous computational overload which may be caused in large and diverse document collections such as pages downloaded from the World Wide Web. In order to reduce computation costs and space complexity, many popular methods for clustering web documents, including those using inverted files, usually assume a relatively small prefixed number of clusters.We propose several new crisp and fuzzy approaches based on the cosine similarity principle for clustering documents that are represented by variable-size vectors of key phrases, without limiting the final number of clusters. Each entry in a vector consists of two fields. The first field refers to a key phrase in the document and the second denotes an importance weight associated with this key phrase within the particular document. Removing the restriction on the total number of clusters, may moderately increase computing costs but on the other hand improves the method’s performance in classifying incoming vectors as normal or abnormal, based on their similarity to the existing clusters. All the procedures represented in this work are characterized by two features: (a) the number of clusters is not restricted by some relatively prefixed small number, i.e., an arbitrary new incoming vector which is not similar to any of the existing cluster centers necessarily starts a new cluster and (b) a vector with multiple appearance n in the training set is counted as n distinct vectors rather than a single vector. These features are the main reasons for the high quality performance of the proposed algorithms. We later describe them in detail and show their implementation in a real-world application from the area of web activity monitoring, in particular, by detecting anomalous documents downloaded from the internet by users with abnormal information interests. 相似文献
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Three experiments were conducted to show that engaging in an effort to answer a question markedly influences the processing of information subsequently encountered in the environment. On each of 2 successive days, participants were given a definition test that involved the recall of rare English words cued by their definitions. A lexical-decision task was administered between the 2 definition tests; the timing of the lexical-decision task varied across experiments. A strong target-to-definition priming effect occurred such that prior exposure to words during the lexical-decision task increased their likelihood of being recalled in the final definition test. It also appears that earlier attempts to answer questions affected the acquisition of relevant new material in long-term memory. The role of these priming effects is discussed with respect to the process of updating memory representations. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
66.
Brick Y Boag A 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2011,58(11):2405-2417
A fast non-iterative algorithm for the solution of large 3-D acoustic scattering problems is presented. The proposed approach can be used in conjunction with the conventional boundary element discretization of the integral equations of acoustic scattering. The algorithm involves domain decomposition and uses the nonuniform grid (NG) approach for the initial compression of the interactions between each subdomain and the rest of the scatterer. These interactions, represented by the off-diagonal blocks of the boundary element method matrix, are then further compressed while constructing sets of interacting and local basis and testing functions. The compressed matrix is obtained by eliminating the local degrees of freedom through the Schur's complement-based technique procedure applied to the diagonal blocks. In the solution process, the interacting unknowns are first determined by solving the compressed system equations. Subsequently, the local degrees of freedom are determined for each subdomain. The proposed technique effectively reduces the oversampling typically needed when using low-order discretization techniques and provides significant computational savings. 相似文献
67.
Dror Shamir Yaniv Wolfer Alexander I. Shames Yael Albo Ariela Burg 《Israel journal of chemistry》2020,60(5-6):557-562
Monovalent nickel is used as a catalyst in a diversity of important chemical systems due to its properties as a strong reducing agent. However, monovalent nickel is unstable in homogeneous systems; its lifetime is very short, and therefore, its efficiency as a catalyst is quite limited. In this article, we demonstrate the catalytic capabilities of Ni(I)(1,4,8,11-tetraazacyclotetradecane)+ as a reducing and catalytic agent by its entrapment in the silica sol gel matrix. The EPR results indicate that the sol-gel matrix plays two roles: it inhibits the common mechanism of Ni(I)L+ decomposition in aqueous solutions, and it also facilitates its functionality as a heterogeneous catalyst, by stabilizing it for at least several hours. The activity of Ni(I)L+ which is entrapped within the matrix, was studied by reducing 2,2-Bis(bromomethyl)-1,3-Propanediol and I3−. The results indicate that Ni(I)L+/Ni(III)L−H2+ acts as a good reducing agent. These results have important implications for a variety of essential catalytic reactions by shedding light on a new feature of sol-gel matrices and their use as active species stabilizers. 相似文献
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Frosch Dominick L.; Saxbe Darby; Tomiyama A. Janet; Glenn Beth A.; Low Carissa A.; Hanoch Yaniv; Motivala Sarosh J.; Meeker Daniella 《Canadian Metallurgical Quarterly》2010,29(5):555
Objective: We conducted a citation analysis to explore the impact of articles published in Health Psychology and determine whether the journal is fulfilling its stated mission. Design: Six years of articles (N = 408) representing three editorial tenures from 1993–2003 were selected for analysis. Main Outcome Measures: Articles were coded for several dimensions enabling examination of the relationship of article features to subsequent citations rates. Journals citing articles published in Health Psychology were classified into four categories: (1) psychology, (2) medicine, (3) public health and health policy, and (4) other journals. Results: The majority of citations of Health Psychology articles were in psychology journals, followed closely by medical journals. Studies reporting data collected from college students, and discussing the theoretical implications of findings, were more likely to be cited in psychology journals, whereas studies reporting data from clinical populations, and discussing the practice implications of findings, were more likely to be cited in medical journals. Time since publication and page length were both associated with increased citation counts, and review articles were cited more frequently than observational studies. Conclusion: Articles published in Health Psychology have a wide reach, informing psychology, medicine, public health and health policy. Certain characteristics of articles affect their subsequent pattern of citation. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献