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排序方式: 共有866条查询结果,搜索用时 15 毫秒
51.
A Seth I Ourmanov MJ Kuroda JE Schmitz MW Carroll LS Wyatt B Moss MA Forman VM Hirsch NL Letvin 《Canadian Metallurgical Quarterly》1998,95(17):10112-10116
The utility of modified vaccinia virus Ankara (MVA) as a vector for eliciting AIDS virus-specific cytotoxic T lymphocytes (CTL) was explored in the simian immunodeficiency virus (SIV)/rhesus monkey model. After two intramuscular immunizations with recombinant MVA-SIVSM gag pol, the monkeys developed a Gag epitope-specific CTL response readily detected in peripheral blood lymphocytes by using a functional killing assay. Moreover, those immunizations also elicited a population of CD8+ T lymphocytes in the peripheral blood that bound a specific major histocompatibility complex class I/peptide tetramer. These Gag epitope-specific CD8+ T lymphocytes also were demonstrated by using both functional and tetramer-binding assays in lymph nodes of the immunized monkeys. These observations suggest that MVA may prove a useful vector for an HIV-1 vaccine. They also suggest that tetramer staining may be a useful technology for monitoring CTL generation in vaccine trials in nonhuman primates and in humans. 相似文献
52.
The fine specificity of the cellular immune response to Candida albicans (i.e., recognition of different antigenic components) between normal controls and human immunodeficiency virus-infected patients in various stages of disease was compared. C. albicans-specific T cells, enriched by antigen stimulation and interleukin-2 expansion, were challenged with antigenic fractions of different molecular weight obtained by SDS-gel fractionation of C. albicans extracts in the presence of autologous mononuclear cells as antigen-presenting cells. Proliferative responses showed similar patterns of reactivity between controls and category A and B seropositive subjects. Category C patients with concurrent C. albicans infections did not give rise to C. albicans-specific T cell lines, confirming the T cell defect. Patients without clinically evident C. albicans infection had a low but broad reactivity pattern of C. albicans-specific T cells. These results suggest that depletion of C. albicans-specific T cells, independent of their fine specificity, occurs along with disease progression. 相似文献
53.
A polyorganosiloxane retaining a SiO4 tetrahedral framework has been synthesized by the reaction of an inosilicate mineral (para-wollastonite) with a trimethylsilylating reagent. The glassy product dissolved in a wide range of organic solvents but was insoluble in water. The analyses of the product by means of infra-red spectroscopy, X-ray powder diffraction and elemental analysis indicated that trimethylsilyl side groups were linked to the SiO4 framework. The thermal stability of the product was determined by thermal analysis. 相似文献
54.
Kamino T Yaguchi T Kuroda Y Ohnishi T Ishitani T Miyahara Y Horita Z 《Journal of electron microscopy》2004,53(5):459-463
Transmission electron microscopy (TEM) samples of an Mg-Al alloy has been prepared using a Ga-focused ion beam (FIB) milling at two different operating voltages of 10 kV and 40 kV to investigate the influence of the FIB energy on the sample quality. The fine structures of the samples have been studied using a high resolution TEM, and the concentration of the implanted Ga was analysed using an energy dispersive X-ray (EDX) analysis. The result of the TEM observation revealed that point defects were introduced to the sample finally milled at 40 kV but not at 10 kV. However, crystal lattice images and electron diffraction patterns were clearly observed on both the samples. The typical influence of the FIB energy was indicated in the elemental analysis. The relative Ga concentration in the thin sample finally milled at 10 kV was 1.0-2.0 at% that is less than half of 4.0-6.0 at% of the Ga concentration in the sample finally milled at 40 kV. A comparison between the experimental results of the Ga concentration measurement with simulation was also discussed. 相似文献
55.
Satoru Kuroda 《Computational Complexity》2004,13(1-2):69-89
We characterize iterated log depth circuit classes between AC0 and AC1 by Cobham-like bounded recursion schemata. We also give alternative characterizations which utilize the safe recursion method developed by Bellantoni and Cook. 相似文献
56.
57.
Takahashi K Sakano H Rytting JH Numata N Kuroda S Mizuno N 《Drug development and industrial pharmacy》2001,27(2):159-164
The influence of pH on the permeability of p-toluidine (pKa, 5.3) and aminopyrine (pKa, 5.0) through shed snake skin as a model membrane was studied. The pH was adjusted to several values, and the solubility of the drugs in each donor was measured. Flux rates and permeability coefficients were calculated from the steady-state penetration portions. The flux rates of p-toluidine decreased as the pH value in the donor solution increased. On the other hand, the flux rates of aminopyrine were constant at any pH value. The permeability coefficients of each drug increased as the pH value in the donor solution increased. The partition coefficients (octanol/buffer) of each drug were dependent on the molecular fraction of un-ionized species. From these results, it is suggested that ionized species of p-toluidine transports through shed snake skin, but the ionized species of aminopyrine does not. 相似文献
58.
Inductive coupling among stacked chips in a package enables 1 Gb/s/channel data communications. Array arrangement of the channel increases data bandwidth, while signal may be degraded by crosstalk. In this paper, crosstalk is measured and analyzed, and crosstalk countermeasures are discussed. Received signal waveforms through the inductive coupling are measured by embedded voltage detectors on a test chip. Interference-to-signal ratio (ISR) has good agreement between the measurements and calculations. It is found that crosstalk is reduced negligibly at a certain distance. If the channels are arranged at intervals of this distance, ISR is minimized. A technique based on time interleaving is also proposed to further reduce crosstalk. A 3times17 channel array is implemented with the crosstalk countermeasures. The channel pitch is taken down to 50 mum. Inter-chip communication with data rate of 1 Gb/s/channel and bit error rate (BER) lower than 10-9 is demonstrated 相似文献
59.
A hybrid tabletop (2 m x 3 m) terawatt chirped-pulse amplification Ti:sapphire-Nd:glass laser (1054 nm, 475 fs, 500 mJ, 9 x 10(17) W cm(-2)) has been developed for laser-matter experiments. An overall gain factor of 10(10) was achieved for the laser. The results of laser applications in the studies of soft-x-ray Ni-like Mo 18.9-nm lasing and of harmonic generation from solid surfaces are presented. 相似文献
60.
Kiyonobu Ida Masanori TomonariYasuyuki Sugiyama Yuki ChujyoTomoharu Tokunaga Tetsu YonezawaKotaro Kuroda Katsuhiro Sasaki 《Thin solid films》2012,520(7):2789-2793
Cu nanoparticle ink was prepared from Cu nanoparticles that were coated with a gelatin layer at an average diameter of 46 nm. The Cu nanoparticle ink was applied on the polyimide substrate. Conductive films were fabricated using the Cu nanoparticle ink with a two-step annealing process consisting of oxidative pre-heating at 200 °C under 10 ppm O2-N2 mixed gas flow and reductive calcination at 250 °C under 3 vol.% H2-N2 mixed gas flow showed a low resistivity of 5 μΩ cm. The hydrolysis of the remaining gelatin layer by H2O vapor, which was formed during the reduction of the Cu oxide by 3 vol.% H2-N2 mixed gas, was suggested. The results suggest the possibility of the removal of the gelatin layer without oxidative pre-heating and simultaneous sintering of Cu nanoparticles in reductive calcination. 相似文献