Production of horse radish peroxidase by plant cell culture

Calluses were induced from the stem and the root tissues of horse radish plantlets. Cell aggregate selections were carried out on them to obtain a cell line with higher peroxidase activity, and the isozyme patterns of the peroxidases from the cultured cells and their utility values as a clinical diagnostic reagent were investigated. The repetition of the cell aggregate selection resulted in increases in peroxidase activities in each generation of selection; about eleven fold in the 13th generation of selection from the stem tissues and about nine fold in the 13th generation from the root tissues. One of the cell lines (N5K6-S), originally derived from the stem tissues, after selectin showed a tenfold increase in peroxidase activity within two weeks of culture in a liquid medium. The value as a clinical diagnostic reagent of peroxidases extracted from the N5K6-S cells was estimated to be equivalent to those already commercially available for the same purpose. There were significant differences in the isoenzyme patterns of the peroxidases from root tissues and those from cultured cells. From these results, the possibility of commercial production of peroxidase utilizing plant tissue and cell culture techniques was discussed.     

Prognosis of occlusive cerbrovascular diseases in normotensive and hypertensive subjects

Comparison of the clinical features, especially prognosis, in cerebral infarction was made between nine normotensive subjects and 16 hypertensive patients with an 80% stenosis or occlusion of the intracranial or extracranial arteries. Our own criteria for evaluating hypertension were employed on the basis of the following items: a past history of hypertension, blood pressure levels on admission and during hospitalization, degree of retinopathy, and ECG changes. In 17 of 25 cases, brain circulation was measured by the intravenous RISA technique. Abnormalities of the EEG and reduction of cranial blood flow were greater, and an early prognosis for neurological deficits in the first two months after the onset of stroke was poorer in the hypertensive group than inthe normotensive group. These results are contradictory to the observations of others.     

Experimental study on house cooling and heating with solar energy using flat plate collector

The OSE Project aims at developing the technology to utilize solar energy for heating, cooling and hot water supply on the basis of various technology for energy conservation in buildings. For the first step of this project a solar heating and cooling system with flat plate collectors and absorption refrigeration machine was installed in a house in 1974. This paper reports outlines of the system and operating results.     

Contact force detection on a minute aperture mounted optical contact slider using an acoustic emission sensor

Advances in the digital network society require both higher density and higher transfer rates in all sorts of storage systems. Especially in optical recording, the trend toward higher density and larger capacity requires novel surface-recording technologies that can drastically diminish head-to-medium spacing, resulting in an improvement in spatial resolution and, finally, a higher recording density. To this end, we have already proposed a novel contact optical head slider that is able to almost cancel the suspension load by generating hydrodynamic pressure, thus realizing a lower net contact force. Evaluating the dynamic contact force is requisite in realizing its stable sliding operation and higher signal readout performance. In this study, a continuous acoustic emission (AE) signal was utilized to detect the dynamic contact force. AE signal modulation was compared with the applied out-of-plane acceleration on a medium generated by a spindle combined piezo-electric actuator. It was clarified that the detected AE modulation amplitude was approximately proportional to the applied acceleration, and that utilizing AE modulation will be a useful method for evaluating dynamic contact force.     

Interactive and Probabilistic Proof of Mobile Code Safety

This paper proposes a new proof-based approach to safe evolution of distributed software systems. Specifically, it extends the simple certification mechanism of proof-carrying code (PCC) to make it interactive and probabilistic, thereby devising interactive proof-carrying code (iPCC). With iPCC, a code consumer is convinced, with overwhelming probability, of the existence and validity of a safety proof of a transmitted code through interaction with a code producer. The iPCC mechanism theoretically solves the problem of proof explosion with PCC and can be used to efficiently prove a greater variety of safety properties that may require longer proofs. Technically, the class (PSPACE) of safety properties that are efficiently provable by iPCC is larger than the class (NP) efficiently provable by PCC. To illustrate the power of iPCC, this paper demonstrates that the verification of certain basic safety properties of typical machine instruction codes needs co-NP-complete computation, and shows how these safety properties can be efficiently verified by the iPCC mechanism.This is an extended and revised version of Tsukada (2001a), which appeared in the Proceedings of the 2000 International Symposium on Principles of Software Evolution. A preliminary version was also presented at the International Conference on Advances in Infrastructure for Electronic Business, Science, and Education on the Internet (Tsukada, 2001b).     

In-line type micropulse lidar with an annular beam: experiment

An in-line type compact micropulse lidar (MPL) with an annular beam was developed for low-altitude cloud measurement. An optical circulator and a couple of axicon prisms for an annular beam were installed on the lidar optics. The advantage of using the in-line MPL is its ability to obtain a near-range measurement with a narrow field of view of 0.1 mrad and to obtain a depolarization measurement of the orthogonally polarized echoes caused by ice crystals of a low-altitude cloud. The total insertion loss of the lidar optics was 3 dB. Detectors such as avalance photodiode detectors can be operated in an analog mode near the breakdown voltage because of the high isolation of the optical circulator. The ideal lidar echo variation from the nearest distance was verified by measuring the mountain echoes at various distances. The depolarization measurement of a low-altitude ice cloud was also demonstrated.     

Syntheses of functional condensation polymers. II. A polyamide having epoxy groups and its ring-opening derivatives

Polyamides having epoxy groups and their ring-opening derivatives were prepared and characterized, and some of their properties were investigated. Reaction conditions for the low-temperature polycondensation of cis-2,3-epoxysuccinyl chloride (ESC) with aromatic diamines and the interfacial copolycondensation of ESC and adipyl chloride (AC) with aromatic diamines were established to obtain a high molecular weight polyamide having epoxy groups. In addition, the ring-opening reactions of the epoxy groups in the polyamides were carried out with various amines in order to obtain polyamides having hydrophilic pendent groups such as amino and hydroxyl. It was found that the polyamides prepared by ring-opening reaction of the epoxy group with ethylenediamine (EDA) or ethanolamine (EA) had a higher affinity for moisture than those with hexamethylenediamine (HMD) or n-butylamine (n-BA), which might be ascribed to the distance of hydrophilic groups from the polyamide chain. Those polyamides having hydrophilic pendent groups decomposed upon heating at less than 200°C.     

Determination of sequence length distribution in SBR by ozonolysis-g.p.c. method

Sequence distribution of styrene-butadiene copolymer (SBR) was investigated by ozonolysis-g.p.c. All the double bonds of butadiene units in SBR were cleaved by ozonolysis in methylene chloride at ?30°C, followed by reduction degradation to alcohol with lithium aluminium hydride. The g.p.c. of the ozonolysis products observed with a u.v.-detector showed the peaks corresponding to styrene sequences and styrene-1,2 sequences. Hot- and cold-emulsion SBR samples contained short sequences consisting of 1 to 4 styrene units and 1,2 styrene units respectively. The average sequence length of cold-emulsion SBR was slightly longer than that of hot-emulsion SBR. A partial block SBR prepared by solution polymerization contained short styrene sequences of 1 to 5 units and long sequences having a broad distribution of $\text{M}{}_{\mathrm{w}}\text{M}{}_{\mathrm{n}}\text{= 1.6}$ with the maximum around 35 units. The styrene sequences containing the 1,2 unit was scarcely observed in this sample.