Design, analysis, and implementation of an agent driven pull-based distributed video-on-demand system

The problem of employing multiple servers to serve a pool of clients on a network based multimedia service is addressed. We have designed and practically implemented a prototype system employing multiple servers to render a long duration movie to the customers. We have employed a multiple server retrieval strategy proposed in the literature [39] to realize this system. In the system, server coordination, client behavior and service facilities are completely controlled by an Agent based approach in which we have used the recent Jini technology. Several issues, ranging from data retrieval from individual server, behavior of the underlying network infrastructure, to client management and resource (client buffers) management, are considered in this implementation. We describe in detail our experiences in this complete design process of every module in the software architecture, its purpose, and working style. Further, the system is shown to be robust amidst unpredictable failures, i.e., in the event of server crashes. The load balancing capability is built-in as a safe guard measure to assure a continuous presentation. We present a comprehensive discussion on the software architecture to realize this working system and present our experiences. A system comprising a series of Pentium III PCs on a fast Ethernet network is built as a test-bed. Through this prototype, a wider scope of research challenges ahead are highlighted as possible extensions. Bharadwaj Veeravalli Member, IEEE & IEEE-CS, received his BSc in Physics, from Madurai-Kamaraj Uiversity, India in 1987, Master's in Electrical Communication Engineering from Indian Institute of Science, Bangalore, India in 1991 and PhD from Department of Aerospace Engineering, Indian Institute of Science, Bangalore, India in 1994. He did his post-doctoral research in the Department of Computer Science, Concordia University, Montreal, Canada, in 1996. He is currently with the Department of Electrical and Computer Engineering, Computer and Information Engineering (CIE) division, at The National University of Singapore, Singapore, as a tenured Associate Professor. His main stream research interests include, Multiprocessor systems, Cluster/Grid computing, Scheduling in parallel and distributed systems, Bioinformatics & Computational Biology, and Multimedia computing. He is one of the earliest researchers in the field of divisible load theory. He has published over 75 papers in high-quality International Journals and Conferences. He had secured several externally funded projects. He has co-authored three research monographs in the areas of Parallel and Distributed Systems, Distributed Databases, and Multimedia systems, in the years 1996, 2003, and 2005, respectively. He had guest edited a special issue on Cluster/Grid Computing for IJCA, USA journal in 2004. He has been recently invited to contribute to Multimedia Encyclopedia, Kluwer Academic Publishers, 2005. He is currently serving the Editorial Board of IEEE Transactions on Computers, IEEE Transactions on SMC-A and International Journal of Computers & Applications, USA, as an Associate Editor. He had served as a program committee member and as a session chair in several International Conferences. Long Chen received the B.E. degree in Electrical Engineering and M.E. degree in Electrical Engineering from the Northwestern Polytechnic University, P. R. China, in 1998 and 2001, respectively, and the M.E. degree in Computer Engineering from the National University of Singapore, Singapore, in 2004. He is currently a Ph.D. candidate at the Department of Electrical and Computer Engineering, the University of Delaware, United States. His research interests include multimedia systems, distributed system, network security, and computer architecture.     

A novel optimization-based bandwidth-aware minimum power multicast routing algorithm in green wireless networks

Multicast routing in wireless networks that possess the wireless multicast advantage could significantly reduce the power and energy consumption. However, this kind of multicast routing that only addresses the transmission radius coverage might not be able to meet the bandwidth requirement of the users. As a result, additional transmissions are required to incur more energy consumption and carbon dioxide emissions that make existing algorithms not applicable to bandwidth constrained applications. In this paper, for the first time, we address the bandwidth aware minimum power multicast routing problem in wireless networks where the objective function is to minimize the total power consumption subject to the users?? bandwidth requirements. This problem is a challenging cross-layer design problem that requires seamless and sophisticated integrated design in the network layer (multicast routing) and physical layer (bandwidth-aware wireless transmission and power control). We first formulate this problem as a mixed integer linear programming problem and then propose a Lagrangian relaxation based algorithm to solve this problem. Numerical results demonstrate that the proposed approach is a sound green networking algorithm that outperforms the existing power efficient multicast routing approaches under all tested cases, especially in large bandwidth request, fine radius granularity, large group size and sparse network.     

Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses

Asthma is a chronic respiratory disease with symptoms such as expiratory airflow narrowing and airway hyperresponsiveness (AHR). Millions of people suffer from asthma and are at risk of life-threatening conditions. Lactoferrin (LF) is a glycoprotein with multiple physiological functions, including antioxidant, anti-inflammatory, antimicrobial, and antitumoral activities. LF has been shown to function in immunoregulatory activities in ovalbumin (OVA)-induced delayed type hypersensitivity (DTH) in mice. Hence, the purpose of this study was to investigate the roles of LF in AHR and the functions of dendritic cells (DCs) and Th2-related responses in asthma. Twenty 8-week-old male BALB/c mice were divided into normal control (NC), ovalbumin (OVA)-sensitized, and OVA-sensitized with low dose of LF (100 mg/kg) or high dose of LF (300 mg/kg) treatment groups. The mice were challenged by intranasal instillation with 5% OVA on the 21st to 27th day after the start of the sensitization period. The AHR, cytokines in bronchoalveolar lavage fluid, and pulmonary histology of each mouse were measured. Serum OVA-specific IgE and IgG1 and OVA-specific splenocyte responses were further detected. The results showed that LF exhibited protective effects in ameliorating AHR, as well as lung inflammation and damage, in reducing the expression of Th2 cytokines and the secretion of allergen-specific antibodies, in influencing the functions of DCs, and in decreasing the level of Th2 immune responses in a BALB/c mouse model of OVA-induced allergic asthma. Importantly, we demonstrated that LF has practical application in reducing DC-induced Th2 cell responses in asthma. In conclusion, LF exhibits anti-inflammation and immunoregulation activities in OVA-induced allergic asthma. These results suggest that LF may act as a supplement to prevent asthma-induced lung injury and provide an additional agent for reducing asthma severity.     

Physapruin A Enhances DNA Damage and Inhibits DNA Repair to Suppress Oral Cancer Cell Proliferation

The selective antiproliferation to oral cancer cells of Physalis peruviana-derived physapruin A (PHA) is rarely reported. Either drug-induced apoptosis and DNA damage or DNA repair suppression may effectively inhibit cancer cell proliferation. This study examined the selective antiproliferation ability of PHA and explored detailed mechanisms of apoptosis, DNA damage, and repair. During an ATP assay, PHA provided high cytotoxicity to two oral cancer cell lines (CAL 27 and Ca9-22) but no cytotoxicity to two non-malignant oral cells (HGF-1 and SG). This selective antiproliferation of PHA was associated with the selective generation of reactive oxygen species (ROS) in oral cancer cells rather than in non-malignant oral cells, as detected by flow cytometry. Moreover, PHA induced other oxidative stresses in oral cancer cells, such as mitochondrial superoxide generation and mitochondrial membrane potential depletion. PHA also demonstrated selective apoptosis in oral cancer cells rather than non-malignant cells in annexin V/7-aminoactinmycin D and caspase 3/7 activity assays. In flow cytometry and immunofluorescence assays, PHA induced γH2AX expressions and increased the γH2AX foci number of DNA damages in oral cancer cells. In contrast, the mRNA expressions for DNA repair signaling, including homologous recombination (HR) and non-homologous end joining (NHEJ)-associated genes, were inhibited by PHA in oral cancer cells. Moreover, the PHA-induced changes were alleviated by the oxidative stress inhibitor N-acetylcysteine. Therefore, PHA generates selective antiproliferation, oxidative stress, and apoptosis associated with DNA damage induction and DNA repair suppression in oral cancer cells.     

Systematic reliability analysis of a class of application-specific embedded software framework

Dramatic advances in computer and communication technologies have made it economically feasible to extend the use of embedded computer systems to more and more critical applications. At the same time, these embedded computer systems are becoming more complex and distributed. As the bulk of the complex application-specific logic of these systems is realized by software, the need for certifying software systems has grown substantially. While relatively mature techniques exist for certifying hardware systems, methods of rigorously certifying software systems are still being actively researched. Possible certification methods for embedded software systems range from formal verification to statistical testing. These methods have different strengths and weaknesses and can be used to complement each other. One potentially useful approach is to decompose the specification into distinct aspects that can be independently certified using the method that is most effective for it. Even though substantial-research has been carried out to reduce the complexity of the software system through decomposition, one major hurdle is the need to certify the overall system on the basis of the aspect properties. One way to address this issue is to focus on architectures in which the aspects are relatively independent of each other. However, complex embedded systems are typically comprised of multiple architectures. We present an alternative approach based on the use of application-oriented-frameworks for implementing embedded systems. We show that it is possible to design such frameworks for embedded applications and derive expressions for determining the system reliability from the reliabilities of the framework and the aspects. The method is illustrated using a distributed multimedia collaboration system.     

Resolution enhanced light field near eye display using e‐shifting method with birefringent plate

Light field near eye displays (LFNED) can produce lightweight devices and address the accommodation–convergence conflict. However, low spatial resolution creates a poor immersive experience in LFNED. In addition, although many e‐shifting devices have been proposed to enhance resolution in projection system, yet those devices are too bulky to be applied in an LFNED to keep it lightweight. In this paper, a compact e‐shifting component is proposed to enhance image resolution in an LFNED by using a birefringent plate and twisted nematic switch cell. The proposed e‐shifting device is a flat and thin component with only 2.6 mm of thickness, which could be placed in the gap of an LFNED without increasing the thickness. The results show that the proposed components could be easily integrated in an LFNED with the result of resolution enhancement.     

Comparison of high pressure treatment and thermal pasteurization effects on the quality and shelf life of guava puree

The effects of high pressures and thermal pasteurization on the survival of microorganisms, enzyme inactivation and quality changes of guava puree during storage at 4°C were investigated and compared with untreated samples. After treatment at a pressure of 600MPa and 25°C for 15 min, the microorganisms in guava puree were inactivated to less than 10 cfu mL⁻¹ and the product exhibited no change in colour, pectin, cloud and ascorbic acid content as compared with fresh samples. The inactivation of enzymes in guava puree by thermal pasteurization was greater than by high pressures. The microbial count in guava puree reduced to 200 cfu mL⁻¹ and the product showed marked changes in viscosity, turbidity and colour when heated at 88–90°C for 24s. The content of pectin, cloud and ascorbic acid as well as colour in untreated and high pressurized (400MPa) guava puree gradually decreased, whereas these changes were not observed in pasteurized (88–90°C) and high pressurized (6000MPa) puree during storage at 4°C for 60 days. The guava puree treated at 600MPa and 25°C for 15 min retained good quality similar to the freshly extracted puree after storage at 4°C for 40 days.     

Growth inhibitory efficacy of lycopene and β-carotene against androgen-independent prostate tumor cells xenografted in nude mice

Scope : In this study, we evaluated the efficacy of lycopene against the growth of prostate cancer in vivo. Methods and results : Athymic nude mice were implanted subcutaneously with human androgen‐independent prostate carcinoma PC‐3 cells. They were supplemented with a low or a high dose of lycopene (4 and 16 mg/kg) and a single dose of β‐carotene (16 mg/kg) twice a week for 7 wk. At the end of the experiment, both lycopene and β‐carotene strongly inhibited the tumor growth, as evidenced by the decrease in tumor volume and tumor weight. High‐dosage lycopene and β‐carotene significantly decreased the expression of proliferating cell nuclear antigen in tumor tissues and increased the levels of insulin‐like growth factor‐binding protein‐3 in plasma. In addition, high‐dosage lycopene supplementation significantly decreased the vascular endothelial growth factor (VEGF) levels in plasma. In contrast, β‐carotene supplementation significantly increased the VEGF levels, as compared with tumor control group. Conclusion : Lycopene and β‐carotene supplementation suppressed the growth of prostate tumor cells, and the effects are likely associated with reduction of proliferation (attenuation of proliferating cell nuclear antigen expression) and with interference of the insulin‐like growth factor 1 signaling (increased plasma insulin‐like growth factor‐binding protein‐3 levels). Furthermore, the inhibition of VEGF by lycopene suggests that the antitumor mechanisms of lycopene also involve anti‐angiogenesis.     

Myrosinase activity and total glucosinolate content of cruciferous vegetables,and some properties of cabbage myrosinase in Taiwan

The myrosinase activity and total glucosinolates of 10 different cruciferous vegetables and some properties of cabbage (Brassica oleracea L var capitata L) myrosinase were investigated. Radish, cabbage and broccoli showed higher myrosinase activity than other samples. No correlation (P > 0–05) was found between myrosinase activity and total glucosinolates of cruciferous vegetables tested. Myrosinase activity for white cabbage was higher than red cabbage. An optimal pH of myrosinase activity was observed at pH 8–0 for both white and red cabbages. The myrosinase extracted from cabbage was more stable in neutral or alkaline pH. The optimal temperature of myrosinase activity for both cabbages was about 60°C, but the myrosinase activity was destroyed after heating at 70°C for 30 min. The myrosinase activities of both white and red cabbages were activated by 10 mM and 5 mM ascorbic acid, respectively.     

Using crude glycerol and thin stillage for the production of microbial lipids through the cultivation of Rhodotorula glutinis

Single cell oils (SCO) produced from oleaginous microorganisms are a potential alternative oil feedstock for biodiesel production. The worldwide production of glycerol, a 10% (w/w) byproduct produced in the transesterfication process of oils converted to biodiesel, is increasing as more biodiesel is being produced. For the purposes of cost reduction, crude glycerol was regarded as a suitable carbon source for the cultivation of Rhodotorula glutinis. In addition to using renewable crude glycerol, waste solution collected from the brewing company (called thin stillage) was adopted as a substitute to replace a costly nitrogen source used in the medium. The results of using mixture of crude glycerol and thin stillage indicated about a 27% increase in total biomass as compared to that of using crude glycerol with a standard medium. Using glycerol instead of glucose as the carbon source could also alter the lipid profile, resulting in an increase in linolenic acid (C18:2) to comprise over 20% of the total lipid. Successfully using renewable crude glycerol and thin stillage for the cultivation of oleaginous microorganisms could greatly enhance the economic competition of biodiesel produced from SCO.