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211.
Takayuki Ueki Akifumi Yoshihara Yuji Teramura 《Science and Technology of Advanced Materials》2016,17(1):807-815
Since circulating tumor cells (CTCs) are tumor cells which are found in the blood of cancer patients, CTCs are potential tumor markers, so a rapid isolation of CTCs is desirable for clinical applications. In this paper, a three-dimensional polystyrene (PS) microfiber fabric with vacuum aspiration system was developed for capturing CTCs within a short time. Various microfiber fabrics with different diameters were prepared by the electrospinning method and optimized for contact frequency with cells. Vacuum aspiration utilizing these microfiber fabrics could filter all cells within seconds without mechanical damage. The microfiber fabric with immobilized anti-EpCAM antibodies was able to specifically capture MCF-7 cells that express EpCAM on their surfaces. The specificity of the system was confirmed by monitoring the ability to isolate MCF-7 cells from a mixture containing CCRF-CEM cells that do not express EpCAM. Furthermore, the selective capture ability of the microfiber was retained even when the microfiber was exposed to the whole blood of pigs spiked with MCF-7 cells. The specific cell capture ratio of the vacuum aspiration system utilizing microfiber fabric could be improved by increasing the thickness of the microfiber fabric through electrospinning time. 相似文献
212.
Shuang Bai Tetsuya Matsumoto Yoshinori Takeuchi Hiroaki Kudo Noboru Ohnishi 《Machine Vision and Applications》2013,24(5):959-970
In image classification based on bag of visual words framework, image patches used for creating image representations affect the classification performance significantly. However, currently, patches are sampled mainly based on processing low-level image information or just extracted regularly or randomly. These methods are not effective, because patches extracted through these approaches are not necessarily discriminative for image categorization. In this paper, we propose to utilize both bottom-up information through processing low-level image information and top-down information through exploring statistical properties of training image grids to extract image patches. In the proposed work, an input image is divided into regular grids, each of which is evaluated based on its bottom-up information and/or top-down information. Subsequently, every grid is assigned a saliency value based on its evaluation result, so that a saliency map can be created for the image. Finally, patch sampling from the input image is performed on the basis of the obtained saliency map. Furthermore, we propose a method to fuse these two kinds of information. The proposed methods are evaluated on both object categories and scene categories. Experiment results demonstrate their effectiveness. 相似文献
213.
为了解决海洋数据的多源异构问题,使用户对大量复杂的海洋数据进行有效管理,设计并实现一套海洋信息管理系统。首先介绍系统的体系结构,然后对各模块进行详细的阐述,并对海洋数据进行了详细的分析。针对海洋数据存在数据不一致、数据空缺和数据冗余等问题,使用基于累积变化量的时间序列异常检测方法,有效检测出海洋数据中的异常点,再对异常点进行修正,保证了海洋数据的质量,从而建立一套完善的海洋信息管理系统,使得海洋相关部门的业务流程更加科学化和规范化。 相似文献
214.
Q. J. Liu T. Takamura N. Takeuchi G. Shao 《International journal of remote sensing》2013,34(17):3385-3405
The Changbai Mountain Natural Reserve (2000 km 2 ), north-east China, is a very important ecosystem representing the temperate biosphere. The cover types were derived by using multitemporal Landsat TM imagery, which was modified with DEM data on the relationship between vegetation distribution and elevation. It was classified into 20 groups by supervised classification. By comparing the results of the classification of different band combinations, bands 4 and 5 of an image from 18 July 1997 and band 3 of an image from 22 October 1997 were used to make a false colour image for the final output, a vegetation map, which showed the best in terms of classification accuracy. The overall accuracy by individual images was less than 70%, while that of the multitemporal classification was higher than 80%. Further, on the basis of the relationship of vegetation distribution and elevation, the accuracy of multitemporal classification was raised from 85.8 to 89.5% by using DEM. Bands 4 and 5 showed a high ability for discriminating cover types. Images acquired in late spring and mid-summer were recognized better than other seasons for cover type identification. NDVI and band ratio of B4/B3 proved useful for cover type discrimination, but were not superior to the original spectral bands. Other band ratios like B5/B4 and B7/B5 were less important for improving classification accuracy. The changes of spectral reflectance and NDVI with season were also analysed with 10 images ranging from 1984 to 1997. Seperability of images in terms of classification accuracy was high in late spring and summer, and decreased towards winter. There were five vegetation zones on the mountain, from the base to the peak: deciduous forest zone, mixed forest zone, conifer forest zone, birch forest zone and tundra zone. Spruce-fir conifer dominated forest was the most dominant vegetation (33%), followed by mixed forest (26%), Korean pine forest (8%) and mountain birch forest (5%). 相似文献
215.
Large part of urban area of around Kobe city were damaged by the 1995 Hyogoken-nanbu earthquake. For detecting the damaged area, the authors computed correlation coefficients from single-look complex ERS-1/SAR data. Two types of correlations, intensity correlation and coherence were computed. Decorrelation was found in the damaged urban built-up area in the data pair of which interval included the earthquake occurrence. The similarity of the decorrelation between the intensity correlation and the coherence indicated that major factor of the decorrelation is closely related to interferometric processes, which results in the change of speckle patterns in the single-look intensity images. A normalized difference was calculated from the correlation coefficients between the data pair including the earthquake occurrence and the pair before the earthquake. The distribution patterns of the pixels for which normalized difference was higher than a threshold showed good correspondence with the result of the ground survey. The result of this study indicates the possibility of detecting urban disasters using the decorrelation of SAR data. 相似文献
216.
217.
Hirotaka SoHirofumi Watanabe Masayuki YahiroYu Yang Yuji OkiChihaya Adachi 《Optical Materials》2011,33(6):755-758
In this study, we synthesized the new spirobifluorene derivatives; hexylbiphenyl-spirobifluorene (HBP-Spiro) and triphenylamine-spirobifluorene (TPA-Spiro) and demonstrated the operation of a distributed-feedback polymer waveguide blue laser. In addition, we investigated the optical and lasing properties of spirobifluorene derivatives including photostability. The experimental slope efficiency and the energy threshold of a HBP-Spiro-doped waveguide laser were 3.6% and 1.9 μJ, respectively. An operation lifetime of 27,000 shots was obtained for a pump-pulse duration of 1.2 ns and energy of 3.0 μJ at a wavelength of 355 nm. 相似文献
218.
Naka R. Watanabe K. Kawarabayashi J. Uritani A. Iguchi T. Hayashi N. Kojima N. Yoshida T. Kaneko J. Takeuchi H. Kakuta T. 《IEEE transactions on nuclear science》2001,48(6):2348-2351
The purpose of this study is to develop a radiation distribution monitor using a normal plastic optical fiber. The monitor has a long operating length and can obtain continuous radiation distributions. A principle of the position sensing is based on a time-of-flight technique. The monitor is sensitive to beta rays or charged particles, gamma rays, and fast neutrons. The spatial resolutions for beta-rays ( 90Sr-90Y), gamma-rays (137Cs), and D-T neutrons are 30, 37, and 13 cm, respectively. The detection efficiencies for the beta-rays, gamma-rays, and D-T neutrons are 0.11%, 1.6×10 -5% and 1.2×10-4%, respectively. The effective attenuation length of the detection efficiency is 18 m. In this paper, we describe the basic characteristics of this monitor 相似文献
219.
Takeuchi K. Satoh S. Tanaka T. Imamiya K. Sakui K. 《Solid-State Circuits, IEEE Journal of》1999,34(5):675-684
A new, negative Vth cell architecture is proposed where both the erased and the programmed state have negative Vth. This architecture realizes highly scalable, excellently noise-immune, and highly reliable NAND flash memories. The program disturbance that limits the scaling of a local oxidation of silicon (LOCOS) width in a conventional NAND-type cell is drastically reduced. As a result, the scaling limit of the LOCOS width decreases from 0.56 to 0.45 μm, which leads to 20% isolation width reduction. The proposed cell is essential for the future scaled shallow trench isolated cells because improved program disturb characteristics can be obtained irrespective of the process technology or feature size. New circuit techniques, such as a PMOS drive column latch and a Vcc-bit-line shield sensing method are also utilized to realize the proposed cell operation. By using these novel circuit technologies, array noise, such as a source-line noise and an inter bit line capacitive coupling noise, are eliminated. Consequently, the Vth fluctuation due to array noise is reduced from 0.7 to 0.1 V, and the Vth distribution width decreases from 1.2 to 0.6 V. In addition to the smaller cell size and the high noise immunity, the proposed cell improves device reliability. The read disturb time increases by more than three orders of magnitude, and a highly reliable operation can be realized 相似文献
220.
Sugita Y. Suzuki R. Ikeda T. Takeuchi T. Kodama N. Takeshita M. Imura R. Satoh T. Umezaki H. Koyama N. 《IEEE transactions on magnetics》1986,22(4):239-246
Hybrid bubble memory devices have been proposed and operated with the memory density of 4 Mbit/cm2. In the hybrid bubble memory devices, minor loops are composed of ion-implanted tracks with 4-µm period, and major lines and functional parts including block-replicate and swap gates are composed of Permalloy tracks with a longer period of 12 µm. Passive junctions between ion-implanted and Permalloy tracks have been developed, introducing the tapered ion-implantation technique. Improving the characteristics of the functional parts composed of Permalloy tracks, the hybrid bubble memory devices with block-replicate and swap gates have been operated, and the feasibility of the devices has been confirmed. In addition, the possibility of higher memory density has been shown. 相似文献