温州市单核细胞增生李斯特菌的毒力基因及血清学分型和分子分型研究

目的了解温州市近十年单核细胞增生李斯特菌分离株的血清型、毒力基因及分子分型特征。方法用聚合酶链式反应(PCR)方法对单核细胞增生李斯特菌进行血清型及毒力基因检测;用多位点序列分型(MLST)方法对单核细胞增生李斯特菌进行分子分型,并绘制MLST数据的最小生成树。结果 97株单核细胞增生李斯特菌分离株分为4种血清型,以血清型1/2b、1/2a为优势血清型,占比分别为48.45%(47/97)、35.05%(34/97);而毒力基因iap、prfA基因阳性率均为100.00%(97/97),hlyA、inlA基因阳性率均为97.94%(95/97),plcB基因阳性率为96.91%(94/97)。其中患者分离株5种毒力基因阳性率均为100.00%(6/6)。97株单核细胞增生李斯特菌分离株得到20个MLST型别,其中ST87型是优势型别,其次为ST121和ST9,ST1和ST779型是患者特有的,ST2、ST3、ST5型分布于食品和患者分离株。结论温州市不同来源的单核细胞增生李斯特菌分离株分子型别呈多态性,食品和患者分离株存在相同的ST型,且这些菌株大部分携带毒力基因,具有潜在的致病性,因此食品中单核细胞增生李斯特菌污染的潜在风险不容忽视。     

Correlation between clothing air gap space and fabric mechanical properties

To investigate the distribution of clothing air gap layers, clothes with the same pattern but different fabrics were put on a standard scan model. The [TC]² NX-16 three-dimensional (3D) body scanning technique was used to collect point cloud data and the overall 3D visualization model of clothing air gaps was represented in a novel way through computer graphics processing. The volumes of air gap space were calculated. The main fabric mechanical properties were investigated and the relationship among them was analyzed by stepwise regression. The results demonstrated a close relationship between clothing air gap space and fabric mechanical properties. The volumes of air gap space were significantly positively correlated with stiffness-bending length and warp density and significantly negatively correlated with drape-crest angle uniformity. The conclusion could play a role in predicting and controlling the clothing design process, especially the material design process of clothing.     

油罐清洁连体作业服的开发和工效学评价

根据油罐清洁环境和工作要求,以满足防护性为前提,通过功能性结构设计达到服装的合体性与舒适性.利用现有面料设计制作新型油罐清洁连体作业服,特别是腰部、膝部等大幅活动部位以及衣袖裤脚的收口处.通过人体工效学实验检验,5 cm最大打开量的腰部弹性折裥可使新型服装在直立或直身下蹲时保持合体性,轻度弯腰达2.5 cm平均打开量,弯腰抱膝等大幅动作时拉伸服装达4.6 cm的平均量.脚口和袖口的三角插片与拉链收口设计同常规橡筋收口服装在该处的皮肤覆盖程度无显著差异,并可提高舒适性.新型连体服装在所有的实验设计部位的人体活动限制均小于现有连体服,使人体肢体活动更加灵活.为提供集功能性、防护性与合体性、舒适性为一体的油罐清洁连体作业服提供参考.     

Evaluation on an ergonomic design of functional clothing for wheelchair users

Researchers have pointed out that people with physical disabilities find it difficult to obtain suitable clothing. In this study a set of wheelchair user oriented functional clothing was designed. Attention was paid to the wheelchair users' daily living activities related with clothing. An evaluating system combined with sports tournament and rehabilitation medicine was introduced to assess the new designed clothing. Six wheelchair users (3 males and 3 females) were invited to wear the clothing. A set of normal functional clothing was employed as a comparison (Control). The time required to complete three different daily living activities, i.e. dressing and undressing, going to toilet and bathing were recorded. Results showed that with the new clothing wheelchair users' competence of managing toilet was increased by 52.9%. The time needed for toilet was reduced by 45.7%. Their capability of managing dressing and undressing was improved by 24.6%. The study indicated that the newly designed clothing could facilitate wheelchair users' daily living activities related with clothing.     

智能电话查询系统

介绍了一个用于交通信息查询的交互式对话系统-ShanghaiQuest。该系统集成了语音识别、文语转换和自然语言理解等技术，用户可以同这样一个自动的交通代理对话以获取所需的交通信息。     

浅谈工程监理是控制工程造价的有效措施

本文以工程监理控制工程造价的角度 ,较为详尽的阐述了工程监理及控制工程造价在设计、施工竣工结算阶段的质量与价格控制要点     

细水雾灭火技术在厨房设备火灾中的应用和推广

文章介绍了国内外厨房设备食用油引发火灾的几率及火灾对人们生产生活影响和危害,概述了细水雾技术的发展历程,介绍了厨房设备火灾特点及细水雾灭火机理,阐述了厨房设备细水雾灭火技术的特点及具有良好的技术优势,是清洁、无毒、无污染高效的灭火技术,具有良好的应用和推广价值.     

基于弹塑性限位装置的基础隔震建筑地震碰撞行为

在近场速度脉冲型地震作用下,基础隔震建筑可能产生过大的支座变形,为了防止结构和隔震沟边缘产生刚性碰撞并对上部结构造成损伤,建议在隔震层高度用弹塑性限位装置来防止结构产生过大位移并实现一定程度的消能减震。提出了一组动力学方程,将建筑结构模型和Bouc-Wen单元串联,可以考虑隔震间隙的非线性动力响应。考虑了速度脉冲周期对结构响应的放大作用,根据弹塑性动力碰撞分析方程,以3组不同高度的钢框架结构基准模型为算例,计算了限位装置的弹性刚度和屈服力等因素对不同结构的弹塑性响应。结果表明：恰当选取用于碰撞限位的弹塑性限位装置的碰撞刚度与屈服力,既能有效限制隔震层的水平位移,上部结构也不会产生过大的反向动力响应,能够充分保护结构;在较易激发类共振效应的速度脉冲型地震动作用下,上部结构最大响应同弹塑性限位装置的弹性刚度与屈服力呈正相关性。     

中心城区绿道智能选线规划研究——以宿迁为例

【目的】当前在绿道系统规划分析中较少应用自下而上的人本分析视角和时空间行为分析方法,缺乏对居民在绿道中行为方式的研究,在方法上对于机器学习的应用仍然处于探索阶段。【方法】以经典设计理论作为支撑,选取宿迁市中心城区作为案例地,整合手机信令数据、居民活动数据、街景数据等多源数据,借助人工智能、LSTM神经网络、时空行为分析等量化分析方法,分析居民真实出行的时空特征,提出绿道智能选线分析框架。【结果】结合LSTM神经网络、类A*算法等构建的轨迹模拟模型可有效开展绿道选线评估,模拟居民真实出行行为,提取潜在绿道网络。【结论】构建了中心城区绿道智能选线方法,将居民真实活动轨迹融入空间要素分析中,融合居民出行行为特征与街道环境要素,通过量化评估方法为绿道选线规划和建设提供指导。