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61.
Y Sheng A Sali H Herzog J Lahnstein SA Krilis 《Canadian Metallurgical Quarterly》1996,157(8):3744-3751
beta2-Glycoprotein I (beta2GPI) is a phospholipid-binding serum protein with anticoagulant properties. It plays a vital role in the binding of anti-cardiolipin Abs purified from patients with autoimmune disease when assayed in a cardiolipin (CL) ELISA. Based on a three-dimensional model of beta2GPI, electrostatic calculations, and earlier peptide studies, a highly positively charged amino acid sequence, Lys282-Asn-Lys-Glu-Lys-Lys287, located in the fifth domain of beta2GPI, has been predicted to be the phospholipid binding site. We tested this hypothesis by site-directed mutagenesis of residues in the predicted phospholipid binding site and by assessing the mutants for phospholipid binding and anti-beta2GPI activity. A single amino acid change from Lys286 to Glu significantly decreased the binding of beta2GPI to CL. Double and triple mutants 2k (from Lys286, 287 to Glu286, 287), 2ka (from Lys284, 287 to Glu284, 287), and 3k (from Lys284, 286, 287 to Glu284, 286, 287) possessed no binding of Ab to beta2GPI in a CL ELISA, as well as no inhibitory activity on the binding of iodinated native beta2GPI to CL. These results indicate that the residues Lys284, Lys286, and Lys287 in the fifth domain of beta2GPI are critical for its binding to anionic phospholipids and its subsequent capture for binding of anti-beta2GPI Abs. 相似文献
62.
本文提出一种“利用原色漂移量调整法”确定当物体光谱反射率未知时光源与物体色显色关系。 利用该关系,通过微机真彩色数字图象技术对光环境进行仿真模拟。 相似文献
63.
管线用高强韧性宽厚钢板的开发 总被引:1,自引:0,他引:1
本文回顾了舞钢高强韧性管线用宽厚钢板的研制开发过程,并结合生产工艺,对钢板性能作了全面分析,指导了钢管线钢的生产。舞钢X70级管线钢有较高的断裂韧性、良好的可焊性以及抗SSCC、HIC性能。舞钢正在进行技术改造,以进一步改善组织,提高性能、满足西气东输要求。 相似文献
64.
热处理工艺对冲击曲线的影响 总被引:1,自引:0,他引:1
通过SA515Gr.70钢的不同温度正火和13MnNiMoNbR钢正火不同温度回火试样的示波冲击试验,研究了不同热处理工艺对冲击曲线的影响。 相似文献
65.
66.
A new time finite-element method based on the extrapolation technique and the Galerkin time finite-element method is presented. In this method, the second-order governing differential equations of motion for dynamic problems are rewritten as a set of first order differential equations in state space. The standard Galerkin method is then employed for the temporal discretization. The algorithm is first-order accurate only. Based on the first-order Galerkin time finite-element formulation, the extrapolation technique is introduced to improve the order of accuracy. It is achieved by expressing the numerical amplification matrix of higher-order algorithm as a linear combination of the basic amplification matrices evaluated at selected instances of time. The matrices are combined with different weighting factors. The pairs of the selected instance of time and the corresponding weighting factors are free parameters. Unconditionally stable higher-order accurate formulations can be derived by properly choosing the free parameters. Algorithms up to fourth-order accurate are presented in this paper. Detailed analyses on stability, numerical dissipation and numerical dispersion are also given. Comparisons of the present algorithms with some well-known time-integration methods are presented to demonstrate the versatility of the present method, in particular its accuracy in the higher-order formulations. 相似文献
67.
This paper explores different means of representation for algebraic transductions, i.e., word relations realized by pushdown transducers. The relevance of this work lies more in its point of view rather than any particular result. We are aiming at giving specific techniques for obtaining, or perhaps explaining, decompositions of algebraic (and incidentally, rational) relations, relying solely on their “machine” definition rather than some complex algebraic apparatus. From this point of view, we are hoping to have demystified the heavy formalism employed in the present literature. Some of the novelties of our work are: the use of “stack languages” and “embeddings,” which eliminate the need of arbitrary context-free languages in our characterizations, the study of uniformizations for algebraic transductions and the use of the so-called stack transductions for exposing the anatomy of pushdown transducers.This work was supported by the Natural Science and Engineering Research Council of Canada grants R220259 and OGP0041630. 相似文献
68.
介绍了宝鸡民营钛工业在新材料、新技术、新设备、新应用方面的进展,提供了市场需求与发展前景方面的相关信息,并对中小型钛加工企业的发展提出建议。 相似文献
69.
重点介绍了蓄电池电压陡降与容量之间的关系及各操作因素对电压陡降的影响。由此引出一种新的蓄电池容量预测思路,并就如何实现它进行了初步探讨,最后对该思路进行了简要的展望。 相似文献
70.
张蕴生 《消防技术与产品信息》2005,(2):45-46
从高层医院所应具备自动消防系统和在高层医院日常管理中 ,加强消防安全教育 ,建立管理制度 ,主动发挥“人”主观能动性的两个方面 ,具体阐述了高层医院的消防安全工作 相似文献