Genetic and physical mapping of the WBP1 locus close to CENV.

The WBP1 locus, encoding an essential component of the N-oligosaccharyl transferase, was mapped both genetically and physically. The gene is located on chromosome V between CENV and gcn4. The distance from CENV sequences is 2 kb.     

Comparison of six methylation methods for analysis of the fatty acid composition of albacore lipid

Six methods widely used to produce methyl esters for the gas chromatographic determination of the fatty acid composition of a marine lipid were compared. Four acid-catalyzed methods (1% H₂SO₄: CH₃OH; 5% HCl: CH₃OH; 7% and 14% BF₃: CH₃OH) and two base-catalyzed methods [0.5M NaOCH₃: CH₃OH; (1:4) tetramethylguanidine: CH₃OH] were used.
The use of BF₃: CH₃OH (7% and 14%) gave a lower content of 18:1 n9 than the other methods and produced an artefact (2.7–3.2% of total fatty acid content) eluting between the 20:5 and 24:1 fatty acid methyl esters. No significant differences were obtained between the other four methods.
Accordingly the use of BF₃: CH₃OH for transmethylation of marine lipids is not recommended. Results obtained in the other four methods showed that all are comparable.     

Dough mixing and breadmaking properties of quinoa-wheat flour blends

Flour blends of quinoa-wheat containing 0, 5, 10, 15, and 20% of manually dehulled quinoa meal or flour were evaluated for dough mixing and breadmaking properties and liking of the bread by a small panel. Increasing amounts of quinoa meal or flour increased farinograph absorption and dislike of bread for most of the panel. Dough development time increased with increase in meal but decreased with increase in quinoa flour. At 10% inclusion levels differences in liking from control 100% wheat flour bread were smaller for flour or water extracted meal to most of the panel, than with 10% non-extracted meal. This suggests that 10% inclusion of flour or water extracted meal may have potential for further investigation.     

Electrical conductivity in iodine-doped ethyl cellulose

The electrical conductivity of solution-grown ethyl cellulose (EC) films, 5–30 μm thick, has been studied in the sandwich configuration (metal–EC–metal) as a function of iodine concentration from 0.5 to 5.0 wt% ratio. The studies were conducted in the temperature range 333–383 K, while the field was varied over the range (3.0–5.5) × 10⁴V/cm. Aluminium was used as the lower electrode, while the upper electrode was of Al, Ag, Cu, Au or Sn. Certain transient effects such as a large burst of current immediately after the application of field were observed. An attempt was made to identify the nature of the current by comparing the observed dependence on electric field, electrode material and temperature with the respective characteristic features of the existing theories of electrical conduction. The results show that the electrical conduction follows Ohm's law at lower fields, while at higher fields, space-charge limited current (SCLC) was observed. It was also found that Richardson–Schottky emission was responsible, to some extent, for the transport of charge carriers in the polymer. The conductivity of the films increased on doping with iodine. The dopant molecules are considered to act as additional trapping centes and provide links between the polymer molecules in the amorphous region, thus resulting in the formation of charge transfer complexes.     

Maintaining quality lead-free [circuit board inspection]

The change to lead-free solders means that circuit board inspection will become more important than ever. The author investigates differences between lead and lead-free solders.     

Modification of the method of simulation of truncated random variables

A new method for simulation of truncated random variables is described. Several similar methods are considered. It is shown that a substantial reduction in computation time is achieved by utilizing the proposed method.Translated from Izmeritel'naya Tekhnika, No. 4, pp. 12–13, April, 1995.     

Effect of thermal load on heat-transfer coefficient in porous-sublimation cooling

It was shown that, under thermal loads that are sufficiently larger (smaller) than a certain value, the heat transfer coefficient for porous-sublimation cooling increases (decreases) with an increase in the thermal load.Physico-Technical Low Temperatures Institute, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Inzlieneron-Fizicheskii Zhurnal, Vol. 62, No. 6, pp. 783–785, June, 1992.     

CONSTRUCTION OF ADMISSIBLE OPERATION CONDITION DOMAIN FOR WELL-STIRRED REACTORS DURING THEIR DESIGN AND CONTROL STAGES

The work examines the problem of constructing an admissible operation condition domain for a well-stirred reactor which would satisfy restrictions in conversion, selectivity, heat removal and stability. The domain is very important and must be known during the reactor design and control stages because it yields visual information for analyzing the reactor operations and makes it possible to find the variation intervals of decision variables, necessary to calculate and optimize the reactor. Being known, the admissible operation condition domain makes it possible to estimate the limiting restrictions and to choose the necessary reserves. The domain has completely been studied analytically for chemical processes with arbitrary kinetics.     

Determining the reliability of concrete dams through diagnosis of their bedrock

Power Technology and Engineering -