Boosting the Microbial Electrosynthesis of Acetate from CO2 by Hydrogen Evolution Catalysts of Pt Nanoparticles/rGO

Catalysis Letters - Microbial electrosynthesis (MES) is an effective approach to driving the CO2 reduction to multi-carbon organic products using renewable energy. In this work, the MES of acetate...     

Local Epidermal Endocrine Estrogen Protects Human Melanocytes against Oxidative Stress,a Novel Insight into Vitiligo Pathology

As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H₂O₂ in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H₂O₂-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo.     

Cytological Observations and Bulked-Segregant Analysis Coupled Global Genome Sequencing Reveal Two Genes Associated with Pollen Fertility in Tetraploid Rice

Neo-tetraploid rice with high fertility is a useful germplasm for polyploid rice breeding, which was developed from the crossing of different autotetraploid rice lines. However, little information is available on the molecular mechanism underlying the fertility of neo-tetraploid rice. Here, two contrasting populations of tetraploid rice, including one with high fertility (hereafter referred to as JG) and another with low fertility (hereafter referred to as JD), were generated by crossing Huaduo 3 (H3), a high fertility neo-tetraploid rice that was developed by crossing Jackson-4x with 96025-4x, and Huajingxian74-4x (T452), a low fertility autotetraploid rice parent. Cytological, global genome sequencing-based bulked-segregant (BSA-seq) and CRISPR/Cas9 technology were employed to study the genes associated with pollen fertility in neo-tetraploid rice. The embryo sacs of JG and JD lines were normal; however, pollen fertility was low in JD, which led to scarce fertilization and low seed setting. Cytological observations displayed low pollen fertility (25.1%) and approximately 31.3 and 27.2% chromosome lagging at metaphase I and II, and 28.8 and 24.8% chromosome straggling at anaphase I and II in JD, respectively. BSA-seq of F_2–3 generations and RNA-seq of F₄ generation detected a common fragment, i.e., 18,915,234–19,500,000, at chromosome 7, which was comprised of 78 genes associated with fertility. Among 78 genes, 9 genes had been known to be involved in meiosis and pollen development. Two mutants ny1 (LOC_Os07g32406) and ny2 (LOC_Os07g32040) were generated by CRISPR/Cas9 knockout in neo-tetraploid rice, and which exhibited low pollen fertility and abnormal chromosome behavior. Our study revealed that two unknown genes, LOC_Os07g32406 (NY1) and LOC_Os07g32040 (NY2) play an important role in pollen development of neo-tetraploid rice and provides a new perspective about the genetic mechanisms of fertility in polyploid rice.     

X射线衍射-拉伸法测薄膜应力

薄膜应力的测量一直是一个困难的问题。本文尝试用一种新的方法——X射线衍射-拉伸法,对薄膜的应力进行检测,与侧倾法实验结果相比较,X射线衍射-拉伸法更为准确与可靠。     

Experimental investigation of liquid water in flow field of proton exchange membrane fuel cell by combining X-ray with EIS technologies

The flow field is a pivotal part to manage the transport of water and gas in proton exchange membrane fuel cell. However, the reported water measurement methods (e.g., X-ray and electrochemical impedance spectroscopy (EIS)) cannot give a comprehensive understanding water distribution in the flow field, resulting in challenges in optimizing the channel design and enhancing fuel cell performance. Therefore, we propose a water measurement method combining the X-ray radiography with EIS to investigate the effect of different operating conditions on the growth law and distribution of liquid water in parallel and serpentine flow fields. The attenuation coefficient of liquid water to X-ray is calibrated with constant tube-current and tube-voltage of X-ray generator. Besides, the parallel flow field with hydrophobic treatment is studied. The results show that the water accumulation of the parallel flow field is far more than the serpentine flow field, and the water content of the middle region is higher than that of other regions in the parallel flow field. Furthermore, operating conditions (cathode inlet gas flow rate, inlet gas humidity, and back pressure) have little effect on the liquid water content of the middle region in the parallel flow field. The polarization curve, EIS result, and X-ray radiography show that the performance and water drainage capacity of the hydrophobic parallel flow field are better than the normal one.

     

电子束辐照预硫化技术在轮胎轻量化中的应用

研究电子束辐照预硫化技术在轮胎轻量化中的应用。采用经过电子束辐照预硫化的胎体,可以减薄、减窄过渡层,从而减小轮胎质量,降低生产成本,并且轮胎不会出现胎里露线现象,同时不会影响成品轮胎的充气外缘尺寸、高速性能和耐久性能。     

Supplementation with Docosahexaenoic Acid and Vitamin E Improves Hepatic Triglyceride Accumulation Induced by High-Fat Diet in Mice

The study aims to investigate the effect of combined supplementation with docosahexaenoic acid (C22:6 n-3, DHA) and vitamin E (VE) on the oxidative stress and liver triglycerides (TG) accumulation induced by high-fat diet (HFD) in mice. C57BL/6J mice are fed either a control diet or an HFD for 8 weeks. Animals are supplemented with DHA, VE, or DHA + VE, respectively. Supplementation with DHA alone shows significant improvement in oxidative stress and hepatic steatosis in mice. Supplementation with DHA significantly reduces the liver TG and total cholesterol contents, and the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, compared with the HFD. Supplementation with DHA also significantly decreases the mRNA expression level of sterol regulatory element-binding protein 1C. However, supplementation with VE alone does not show improvement in oxidative stress and hepatic steatosis. DHA + VE supply obtains a superior effect in alleviation of hepatic steatosis than DHA supplementation alone in mice fed by HFD. The efficacy of DHA potentiated by VE can be due to that VE enhances the effect of DHA in decrease of ALT and AST levels and increase of antioxidant enzyme activity and glutathione level in mice fed by HFD. Practical Applications: Supplementation with DHA significantly improves the oxidative stress and hepatic steatosis induced by HFD in mice. The efficacy of DHA in the alleviation of hepatic steatosis induced by HFD is potentiated by VE. These findings may provide a rational basis for the use of DHA and VE co-supplementation in patients with liver steatosis.     

Correction to: Bismuth Yttrium Oxide (Bi3YO6), A New Electrode Material For Asymmetric Aqueous Supercapacitors

Journal of Inorganic and Organometallic Polymers and Materials - The original version of this article unfortunately contained mistakes. In line 9 of the abstract, 5% should read as 2%. The...     

CO2/H2S对油气管材的腐蚀规律

本文综述了CO2、H2S对油气管材的腐蚀机理及影响因素,提出了开发经济型油管的设想.     

Windows NT/2000/XP环境下访问底层I/O端口的方法

不同于Windows 9x/ME操作系统,Windows NT/2000/XP对硬件实现了屏蔽,对I/O端口实行了严格控制,用户不能对底层I/O端口进行直接访问.论文基于利用PortTalk驱动,开发了对I/O端口直接访问的动态链接库,从而成功解决应用软件访问底层物理端口的问题,并用实例说明了该动态库的调用方法.