抗真菌蛋白Rs-AFP2基因定点突变及其对大肠杆菌中表达的影响

为了研究遗传密码子对表达调控的影响,利用PCR重叠延伸法,对萝卜抗真菌蛋白Rs-AFP2基因编码序列区的部分核苷酸进行沉默突变,构建突变体Rs-AFPm.序列分析表明,PCR产物全长240bp,有一个阅读框,编码的蛋白由29个氨基酸的信号肽和51个氨基酸的抗真菌蛋白组成.突变体与突变前的Rs-AFP2基因相比,在编码区第3号氨基酸Lys相差一个碱基(TTG→TTA),第5号氨基酸Gln相差一个碱基(CAG→CAA),第6号稀有密码子Arg相差两个碱基(CAG→CGA).重新合成引物,将切除信号肽的Rs-AFP2基因和Rs-AFPm基因与原核表达载体pET-21b(+)分别重组到大肠杆菌BL21菌株.IPTG诱导后,二者均得到了表达.软件分析显示,突变前pETAFPo表达产物占全菌蛋白的3%,突变后pETAFPm的表达产物占全菌蛋白含量的8%;表达蛋白主要以包涵体的形式存在,包涵体经超声波破碎后,蛋白质复性,抑菌结果表明,pETAFPm表达产物的抑菌半径大于pETAFP2表达产物的抑菌半径.这些都说明改造后的Rs-AFPm基因与Rs-AFP2基因相比,已有效地提高表达量.     

堆石料与基岩面直剪试验

介绍了堆石料与基岩面的现场直剪试验方法，6个组次的直剪试验结果表明，堆石料与基岩面的抗剪强度指标随基岩面坚硬度的增大而增加，并随基岩面粗糙度的加大而加大。     

The Two-Dimensional Clifford-Fourier Transform

Recently several generalizations to higher dimension of the Fourier transform using Clifford algebra have been introduced, including the Clifford-Fourier transform by the authors, defined as an operator exponential with a Clifford algebra-valued kernel. In this paper an overview is given of all these generalizations and an in depth study of the two-dimensional Clifford-Fourier transform of the authors is presented. In this special two-dimensional case a closed form for the integral kernel may be obtained, leading to further properties, both in the L ₁ and in the L ₂ context. Furthermore, based on this Clifford-Fourier transform Clifford-Gabor filters are introduced. AMS subject classification numbers: 42B10, 30G35 Fred Brackx received a diploma degree in mathematics from Ghent University, Belgium, in 1970 and a Ph.D. degree in mathematics from the same university in 1973. Since 1984 he is professor for mathematical analysis at Ghent University and currently he is leading the Clifford Research Group. His main interests are function theory and functional analysis for functions with values in quaternion and Clifford algebras. The research covers Clifford distributions, generalized Fourier, Radon and Hilbert transforms, orthogonal polynomials and multi-dimensional wavelets. Nele De Schepper received a diploma degree in mathematics from Ghent University, Belgium, in 2001. Since then she holds an assistantship at the Department of Mathematical Analysis of Ghent University and is a member of the Clifford Research Group. Her main interests are function theory and functional analysis for functions with values in Clifford algebras. The research covers generalized Fourier transforms, orthogonal polynomials and multi-dimensional wavelets. Frank Sommen received a diploma degree in mathematics from Ghent University, Belgium, in 1978, a Ph.D. degree in mathematics from the same university in 1980, and a habilitation degree in mathematical analysis in 1984. From 1978 until 1999 he was at the National Fund for Scientific Research (Flanders). Since 2000 he holds a Research professorship at Ghent University. His main interests are function theory and functional analysis for functions with values in quaternion and Clifford algebras. The research covers Clifford distributions, generalized Fourier, Radon and Hilbert transforms, orthogonal polynomials and multi-dimensional wavelets, algebraic analysis, hyperfunctions and radial algebra.     

地震数据采集系统延迟时间研究与分析

文章分析了影响地震采集系统延迟时间的因素,并根据系统延迟的理论对产生的原因进行了论证.同时对实际生产中的测试方法进行了阐述研究和分析,提出了新的测试方法,并结合实际生产提出了合理性建议.     

微表处技术在高速公路养护中的应用

结合某高速公路专项治理工程,采用施工用原材料研究了微表处混合料的设计,结合实体工程研究了微表处技术的施工工艺。对微表处的应用效果进行了跟踪观测,研究结论表明沥青路面微表处技术是一种经济、性能优良的高速公路养护技术。     

基于对地观测激光回波特征的目标特性研究

本文研究了对地观测激光回波特征与地面目标特征之间的关系模型，分析了分类地面目标的激光回波特征，提出一种基于查表法的目标特性计算方法并给出了仿真结果。     

不同注F剂量与CMOS运放电路辐照损伤的相关性

在不同注F剂量条件下,对P沟和N沟两种不同差分对输入CMOS运放电路的电离辐照响应进行了研究.分析比较了注F和未注F运放电路电离辐照响应之间的差异.结果表明,在栅场介质注入适量的F,可有效抑制辐照感生的氧化物电荷尤其是界面态的增长,从而提高CMOS运放电路的抗辐照特性.     

ＣＲＳ叠加技术及其应用

地震资料常规处理中的速度分析、动校正、剩余静校正等均是以反射点为基本反射单元进行研究，对于复杂地质界面来说，地下反射点的信息分布在临近多个CDP点上，用常规方法处理无疑会损失许多有用信息。CRS叠加技术是在地震资料处理中利用邻近多个反射点组成的共反射面信息进行动校叠加。介绍了利用CRS叠加技术进行叠加成像及剩余静校正的基本原理及应用。     

魏氏组织对20#碳钢H2S应力腐蚀开裂性能的影响

文章对H₂S-CO₂-H₂O腐蚀环境下发生H₂S应力腐蚀的20^＃碳钢弯管（无缝钢管）进行了成分、组织、拉伸、硬度和应力腐蚀开裂等性能的试验研究，并与同材质、同时投产、腐蚀环境基本相似而未发生应力腐蚀开裂的在役弯管及未使用的新弯管作对比，结果表明魏氏组织是引起20^＃钢发生应力腐蚀开裂的主要原因；正火能消除魏氏组织，降低钢的应力腐蚀开裂敏感性。     

抗乙肝病毒表面抗原PreS1(20-47)的单链抗体在转基因烟草根分泌物中的初步表达

为探索利用植物根分泌表达重组蛋白的可行性，构建了含有抗乙肝病毒表面抗原PreS1(20—47)单链抗体(ScFv)基因的表达载体。该ScFv基因转化烟草后在烟草根部细胞的细胞质和内质网中获得表达。实验结果表明，5’端融合ER导向信号肽的重组ScFv可通过根分泌表达。