Filter matrix estimation in automated DNA sequencing

In four-color fluorescence-based automated DNA sequencing, a 4×4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. Here, the authors present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results     

氢压缩机管道强烈振动的消除

本文针对一往复式氢气压缩机管道振动十分强烈的情况，分析了管系的结构自振频率、气柱固有频率和气流脉动，采取了改变管系结构频率和气柱固有频率的减振措施，经实践证明获得了较为理想的减振效果。     

Identification of cysteine pairs within the amino-terminal 5% of apolipoprotein B essential for hepatic lipoprotein assembly and secretion

There is growing evidence that the amino-terminal globular domain of apolipoprotein B (apoB) is essential for lipoprotein particle formation in the hepatic endoplasmic reticulum. To identify the structural requirements for its function in lipoprotein assembly, cysteine (Cys) pairs required to form the seven disulfide bonds within the amino-terminal 21% of apoB were replaced in groups or individually by serine. Substitution of Cys pairs required for formation of disulfide bonds 1-3 or 4-7 (numbered from amino to carboxyl terminus) completely blocked the secretion of apoB28 in transfected HepG2 cells. To identify the specific disulfide bonds required for secretion, Cys pairs were mutated individually. Substitution of Cys pairs required for disulfide bonds 1, 3, 5, 6, or 7 had little or no impact on apoB28 secretion or buoyant density. In contrast, individual substitution of Cys pair 2 (amino acid residues 51 and 70) or 4 (218 and 234) severely inhibited apoB28 secretion and its capacity to undergo intracellular assembly with lipid. The same assembly and secretion defects were observed when these mutations were expressed as part of apoB50. These studies provide direct evidence that the ability of the internal lipophilic regions of apoB to engage in the recruitment and sequestration of lipid during translation is critically dependent upon a structural configuration contained within or affected by the amino-terminal 5% of the protein.     

模拟信号的恢复及其误差研究

数模混合仿真中模拟信号的恢复及其误差对整个仿真过程具有重要影响。本文探讨了用零阶保持和一阶保持进行信号恢复的方法，比较了它们的误差及工作特点。特别指出了一阶保持中，由于存在积分器复原时间及数字机操作时间的影响而会带来附加的误差，因此一阶保持方法较难达到其理论的精度。     

Magnetization and rotation of MTG HTSC ring in magnetic field

The magnetization of a melt-texture growth (MTG) HTSC ring has been studied. It is shown that the magnetic field inside the ring is larger than the external field under a certain range of external magnetic fields. We have also investigated the magnetic field dependence of the response of a detective coil near a rotating superconducting ring. The responses of the MTG sample are different for different cooling methods.     

A Linear Cross-Coupled Control System for High-Speed Machining

We present a linear cross-coupled controller to improve highspeed contouring accuracy independently of tracking accuracy in a biaxial machine tool feed drive servomechanism. Unlike conventional cross-coupled controllers, the cross-coupled controller presented here is a linear system, so it is very easy to perform the stability and steady-state error analysis, and to optimise the controller parameters. The proposed controller is evaluated experimentally on a CNC LOM machine and compared to an uncoupled controller and a conventional cross-coupled controller. Controller performance is evaluated for a circular contour at a feedrate of 30 m min _1 . The experimental results show that the proposed controller can greatly reduce the contour error at large feedrates. The linear cross-coupled controller is simple to implement and is practical.     

An experience of tunnelling in mudstone area in southwestern Taiwan

Many tunnels will be constructed in southwestern Taiwan in the upcoming decade to meet the huge demands of transportation, energy and water infrastructure projects. Mudstone strata cover more than a 1000 km² area, consisting mainly of massive mudstone or alternation of mudstone and sandy layer, exhibiting unfavorable geological and hydrological characteristics, making it extremely difficult for tunnelling. This paper presents the lessons learned from three tunnelling projects in the 1990s in a mudstone area. In addition to discussing the rock behavior in the vicinity of tunnel and its failure patterns, the monitoring data during tunnelling are presented as well. Finally, the effective method of design and construction are recommended for tunnelling in mudstone area.     

展成式精密电火花加工机床

研制了一种展成式精密电火花加工机床，它含有3个回转机构和4个直线移动机构，工件苡轴，工具电极苡轴和转台均可按需要回转，坐标工作台可作纵向和横向移动，工件头架可作轴向和竖向移动。可设置成多种复合运动方式和相应的结构布局，具有很丰富的加工功能，加工精度能达到微米级并实现电火花镜面加工。     

油田污水中咪唑啉缓蚀剂浓度检测技术研究

研究评价了油田污水中咪唑啉缓蚀剂浓度检测的七种方法 ,其中紫外光分析法和显色反应法适合于低浓度咪唑啉缓蚀剂检测 ,且方法简便、速度快、成本低 ,检测浓度范围 2～ 5 0mg/L。