Neuronal ApoE Regulates the Cell-to-Cell Transmission of α-Synuclein

The presence of protein inclusions, called Lewy bodies (LBs) and Lewy neurites (LNs), in the brain is the main feature of Parkinson’s disease (PD). Recent evidence that the prion-like propagation of α-synuclein (α-syn), as a major component of LBs and LNs, plays an important role in the progression of PD has gained much attention, although the molecular mechanism remains unclear. In this study, we evaluated whether neuronal ApoE regulates the cell-to-cell transmission of α-syn and explored its molecular mechanism using in vitro and in vivo model systems. We demonstrate that neuronal ApoE deficiency attenuates both α-syn uptake and release by downregulating LRP-1 and LDLR expression and enhancing chaperone-mediated autophagy activity, respectively, thereby contributing to α-syn propagation. In addition, we observed that α-syn propagation was attenuated in ApoE knockout mice injected with pre-formed mouse α-syn fibrils. This study will help our understanding of the molecular mechanisms underlying α-syn propagation.     

氢氧发动机多余物防控设计

由于多余物的危害及航天产品高精尖的发展需要,多余物防控设计已成为产品设计不可或缺的设计准则.通过对氢氧发动机多余物的特点进行分析,发动机明确了介质精度控制要求并进行了过滤器的设计,可有效对多余物进行防控.针对氢氧发动机的低温特点,还进行了发动机"潮气及未置换干净的空气"的防控设计以防"冰堵".     

Isolation and Characterization of Urinary Extracellular Vesicles from Healthy Donors and Patients with Castration-Resistant Prostate Cancer

Prostate cancer (PCa) is the most commonly diagnosed malignancy among men in developed countries. The five-year survival rate for men diagnosed with early-stage PCa is approximately 100%, while it is less than 30% for castration-resistant PCa (CRPC). Currently, the detection of prostate-specific antigens as biomarkers for the prognosis of CRPC is criticized because of its low accuracy, high invasiveness, and high false-positive rate. Therefore, it is important to identify new biomarkers for prediction of CRPC progression. Extracellular vesicles (EVs) derived from tumors have been highlighted as potential markers for cancer diagnosis and prognosis. Specifically, urinary EVs directly reflect changes in the pathophysiological conditions of the urogenital system because it is exposed to prostatic secretions. Thus, detecting biomarkers in urinary EVs provides a promising approach for performing an accurate and non-invasive liquid biopsy for CPRC. In this study, we effectively isolated urinary EVs with low protein impurities using size-exclusion chromatography combined with ultrafiltration. After EV isolation and characterization, we evaluated the miRNAs in urinary EVs from healthy donors and patients with CRPC. The results indicated that miRNAs (miR-21-5p, miR-574-3p, and miR-6880-5p) could be used as potential biomarkers for the prognosis of CRPC. This analysis of urinary EVs contributes to the fast and convenient prognosis of diseases, including CRPC, in the clinical setting.     

Investigation on the Transient Thermal Performance of a Mini-Channel Cold Plate for Battery Thermal Management

Cold plate is an important component for a liquid battery thermal management system.In order to study the transient thermal performance of the cold plate under ...     

Effects of Optimized Operating Parameters on Combustion Characteristics and NO_x Emissions of a Burner based on Orthogonal Analysis

To optimize the structure of the burner,improve the combustion performance,and reduce the emission of NO_x,a self-circulating low NO_x combustion technology was used to design a new type of flue gas self-circulating low NO_x burner.Based on previous research on the numerical model of combustion and the composition of mixed gas on combustion and NO_x emissions,the effect of various factors on the ejection coefficient of the flue gas self-circulating structure was analyzed using the orthogonal test method,and the burner operating parameters,such as preheating temperature and excess air coefficient,were deeply studied through the three-dimensional finite element numerical model in this paper.The results show that the diameter ratio of the nozzle and the length of the cylindrical section of the flue gas self-circulating structure have great influence on its ejection and mixing ability.The optimal ejection coefficient was 0.4829.Overall,the amount of NO_x emissions greatly increased from 6.23×10~(-6) (volume fraction) at the preheating temperature 973 K to 3.5×10~(-3) at preheating temperature 1573 K.When the excess air coefficient decreased from 1.2 to 1,the maximum combustion temperature decreased from 2036.3 K to 1954.22 K,and the NO_x emissions decreased from 352.29×10~(-6) to 159.73×10~(-6).     

新型矿用LPTC陶瓷温度传感器

利用复合方法研制出一种ＬＰＴＣ电子陶瓷材料,并经作温度敏感元件,研制出适合井下恶劣环境,精度高、寿命长的温度传感器。并对探头包封和响应时间以及传感器的动、静态特性进行了测量。     

Photoreceptor Cells Constitutively Express IL-35 and Promote Ocular Immune Privilege

Interleukin-27 is constitutively secreted by microglia in the retina or brain, and upregulation of IL-27 during neuroinflammation suppresses encephalomyelitis and autoimmune uveitis. However, while IL-35 is structurally and functionally similar to IL-27, the intrinsic roles of IL-35 in CNS tissues are unknown. Thus, we generated IL-35/YFP-knock-in reporter mice (p35-KI) and demonstrated that photoreceptor neurons constitutively secrete IL-35, which might protect the retina from persistent low-grade inflammation that can impair photoreceptor functions. Furthermore, the p35-KI mouse, which is hemizygous at the il12a locus, develops more severe uveitis because of reduced IL-35 expression. Interestingly, onset and exacerbation of uveitis in p35-KI mice caused by extravasation of proinflammatory Th1/Th17 lymphocytes into the retina were preceded by a dramatic decrease of IL-35, attributable to massive death of photoreceptor cells. Thus, while inflammation-induced death of photoreceptors and loss of protective effects of IL-35 exacerbated uveitis, our data also suggest that constitutive production of IL-35 in the retina might have housekeeping functions that promote sterilization immunity in the neuroretina and maintain ocular immune privilege.     

Experimental investigation on the CO2 laser cutting of soda-lime glass

Journal of Mechanical Science and Technology - This study reports on complete glass cutting using a single CO2 laser beam with a low power of several tens of watts. In this study, the morphological...     

光栅衍射缝间干涉因子次极大强度对称性的菲涅耳半波带法证明

本文用菲涅耳半波带法推导出了一个非常有用的次极大位置公式,将该位置公式和振幅矢量法求出的次极大位置结合起来,方便地求出了缝间干涉因子的次极大的强度,证明了缝向干涉因子的次极大强度具有对称性。避免了对振幅矢量法求次极大位置这个方程的直接求解,因为用该方法求出的次极大强度与理论值完全符合,说明菲涅耳半波带法不但可以用来分析单缝衍射,同时还完全能够用来分析光栅衍射。     

Preparation of Mo2C by MPCVD and Its Photocatalytic Properties

Mo2C was prepared by microwave plasma chemical vapor deposition(MPCVD)technique with the power of 800 W and pressure of 18 kPa. Compared with traditional prepar...