Dysregulated CD38 Expression on Peripheral Blood Immune Cell Subsets in SLE

Given its uniformly high expression on plasma cells, CD38 has been considered as a therapeutic target in patients with systemic lupus erythematosus (SLE). Herein, we investigate the distribution of CD38 expression by peripheral blood leukocyte lineages to evaluate the potential therapeutic effect of CD38-targeting antibodies on these immune cell subsets and to delineate the use of CD38 as a biomarker in SLE. We analyzed the expression of CD38 on peripheral blood leukocyte subsets by flow and mass cytometry in two different cohorts, comprising a total of 56 SLE patients. The CD38 expression levels were subsequently correlated across immune cell lineages and subsets, and with clinical and serologic disease parameters of SLE. Compared to healthy controls (HC), CD38 expression levels in SLE were significantly increased on circulating plasmacytoid dendritic cells, CD14⁺⁺CD16⁺ monocytes, CD56⁺ CD16^dim natural killer cells, marginal zone-like IgD⁺CD27⁺ B cells, and on CD4⁺ and CD8⁺ memory T cells. Correlation analyses revealed coordinated CD38 expression between individual innate and memory T cell subsets in SLE but not HC. However, CD38 expression levels were heterogeneous across patients, and no correlation was found between CD38 expression on immune cell subsets and the disease activity index SLEDAI-2K or established serologic and immunological markers of disease activity. In conclusion, we identified widespread changes in CD38 expression on SLE immune cells that highly correlated over different leukocyte subsets within individual patients, but was heterogenous within the population of SLE patients, regardless of disease severity or clinical manifestations. As anti-CD38 treatment is being investigated in SLE, our results may have important implications for the personalized targeting of pathogenic leukocytes by anti-CD38 monoclonal antibodies.     

离子液体/NMP对双马萃余煤的结构和萃取性能影响

为研究离子液体/NMP对双马萃余煤的结构和萃取性能影响,首先分别用乙醇和丙酮对宁东双马煤样(SM)进行分步萃取,得到萃余煤(SMEC),然后将不同种类的离子液体(ILs)分别与N-甲基吡咯烷酮(NMP)混合后对SMEC进行萃取.用TG研究ILs/NMP萃取前后煤样热分解特性的变化,用FT-IR研究ILs/NMP萃取前后煤样官能团的变化,用GC/MS分析萃取物的种类.结果表明,ILs/NMP萃取后煤样的热失重增大,萃余煤中含氧官能团含量下降.另外,疏水型离子液体/NMP对SMEC煤的累积萃取率大于亲水型离子液体/NMP的累积萃取率.亲水型离子液体[Bmim]Cl/NMP和[Bmim]BF4/NMP分别萃取出51种和44种有机物质;疏水型离子液体[Bmim]NTf2/NMP和[Bmim]PF6/NMP分别萃取出28种和29种有机物质.疏水型离子液体/NMP对SMEC煤中含氧官能团化合物的萃取能力强于亲水型离子液体/NMP;疏水型离子液体/NMP对SMEC煤中含杂原子化合物的萃取能力小于亲水型离子液体/NMP.     

热压烧结Ti3AlC2材料的制备、结构与性能研究

采用热压工艺研究了不同工艺制度和原料中不同的Si含量对Ti3AlC2合成的影响.研究表明在1 300℃～1 500℃,30MPa压力和Ar气氛中热压摩尔比为n(TiC)n(Ti)n(Al)n(Si)=2110.2的混合粉末,可以得到纯度达98%(质量分数)以上的致密块体Ti3AlC2材料;添加的Si均匀分布在基体中,形成固溶体,当添加Si的摩尔比为0.2时,固溶体的化学式为Ti2 76Al0 78Si0.22C2.烧结试样的晶体为层片状结构,1 300℃和1 400℃时,烧结试样的晶粒尺寸分别为10μm～15μm和20μm～30μm.材料的维氏硬度为3.3 GPa～5.0 GPa,弹性模量为289 GPa,抗压强度为785 MPa,抗弯强度为375 MPa,断裂韧性为7.0 MPa·m1/2;25℃时,电导率为3.1×106 S·m-1,热容为125.4 J/mol·K,热导率为27.5 W/m·K;热膨胀系数为8.8×10-6 K-1.     

含硼金刚石的金属包膜成分及其物相的研究

本文采用铁基触媒加入硼铁的方法，在高温高压条件下合成了含硼金刚石，利用电子探针（EPMA），透射电子显微镜（TEM）两种检测手段，分析了含硼金刚石金属包膜的微观组织结构，化学组成成分，各组成成分的物相及其可能形成的原因。研究结果表明：金属包膜的成分及其物相由正交立方结构的（Fe,Ni）3C，面心立方的γ-（Fe,Ni），面心立方的Fe23（C，B）。及少量的FeB,Ni3B组成，这些成份主要来源于原材料，其含量在高温压过程中是瞬间变化的。     

工作流技术在ASP公共服务平台中的应用研究*

根据项目特点,建立了适合ASP平台的基于协调理论和反馈机制的扩展活动网络模型,采用XML模式的XPDL规范实现过程定义;然后通过DOM解析XML数据文档和J2EE的技术支持实现了系统的轻量级工作流引擎.详细介绍了系统实现中解决的两个关键问题:事务型操作的处理和并发控制,并提出了基于优先级的锁绑定机制.     

Coordinated Recovery of Middleware Services:A Framework and Experiments

Being the most popular runtime infrastructure for distributed systems, middleware can be viewed as a collection of common services. Since the development, deployment and maintenance of distributed systems rely largely on middleware services, the failure of middleware services puts a significant impact on the reliability and availability of the whole system. Though recovery-based fault tolerance is an effective way to improve the reliability of middleware services, it is far away from practice mainly because of the high complexity and cost of the recovery of correlated failures between interdependent services. In this paper,a framework for detecting and recovering the correlated failures of middleware services in an automated way is presented. First, the problem is investigated from two perspectives, i.e.,analyzing the role and impact of middleware services and illustrating a set of correlated failures in J2EE standard services as motivating examples. Then, a general coordinated recovery model is constructed with the elements necessary and su±cient for detecting and recovering correlated failures in middleware services. The supporting framework is demonstrated on three J2EE application servers, i.e., PKUAS, JBoss and JOnAS, one by one without fundamental modifications. Finally, based on the three enhanced application servers, many cases on J2EE common services, including the transaction service, database service, naming and directory service, security service and messaging service, are studied. The experiment results show the effectiveness and applicability of the framework presented in this paper.     

500kV输电线路运行稳定性评价模型研究

针对目前500 kV输电线路运维工作任务分配不合理,导致巡检效率低以及线路大修技改采取"差异化"改造方案如何选择规划的问题,建立一种输电线路多维度评价模型.该模型采用模糊综合评价方法,选择与输电线路巡检与大修技改密切相关的多个指标,通过层次分析法与熵权法确定各指标权重,计算各指标对应评语集的隶属度,最终得出评估结果.通过具体案例分析,该模型符合实际情况,能较好地对500 kV输电线路运行稳定性进行评估.     

Genome-Wide Analysis of the Late Embryogenesis Abundant (LEA) and Abscisic Acid-, Stress-, and Ripening-Induced (ASR) Gene Superfamily from Canavalia rosea and Their Roles in Salinity/Alkaline and Drought Tolerance

Canavalia rosea (bay bean), distributing in coastal areas or islands in tropical and subtropical regions, is an extremophile halophyte with good adaptability to seawater and drought. Late embryogenesis abundant (LEA) proteins typically accumulate in response to various abiotic stresses, including dehydration, salinity, high temperature, and cold, or during the late stage of seed development. Abscisic acid-, stress-, and ripening-induced (ASR) genes are stress and developmentally regulated plant-specific genes. In this study, we reported the first comprehensive survey of the LEA and ASR gene superfamily in C. rosea. A total of 84 CrLEAs and three CrASRs were identified in C. rosea and classified into nine groups. All CrLEAs and CrASRs harbored the conserved motif for their family proteins. Our results revealed that the CrLEA genes were widely distributed in different chromosomes, and all of the CrLEA/CrASR genes showed wide expression features in different tissues in C. rosea plants. Additionally, we introduced 10 genes from different groups into yeast to assess the functions of the CrLEAs/CrASRs. These results contribute to our understanding of LEA/ASR genes from halophytes and provide robust candidate genes for functional investigations in plant species adapted to extreme environments.     

氢化物发生-原子荧光光谱法测定铬矿中砷

铬矿中砷的测定方法对完善铬矿检测方法和质量评价手段有重要意义。在0.5 g样品中加入2 g过氧化钠,于650 ℃熔融20 min,熔融物经盐酸酸化溶解,以10%盐酸为载流,15 g/L硼氢化钾溶液为还原剂,采用氢化物发生-原子荧光光谱法(HG-AFS)进行测定,建立了铬矿中砷测定的新方法。实验表明,砷质量浓度在0.025～100 μg/L范围内与荧光强度呈线性关系,相关系数为1.000 0,方法检出限为0.05 μg/g,定量限为0.17 μg/g。对产地为南非、土耳其、巴基斯坦等19个国家共2 649个铬矿样品的成分进行统计,发现铬矿主要成分为铬、铁、镁、铝和硅;干扰试验表明,这些样品中的共存元素均不干扰测定。按照实验方法对3个不同砷含量的铬矿样品进行精密度和加标回收试验,结果的相对标准偏差(RSD,n=11)为1.4%～6.0%,回收率为96%~109%。将实验方法应用于与铬矿基体近似的铁矿标准物质中砷的测定,测定值与认定值基本一致。     

弹簧表层优化喷丸残余应力场的工程计算方法

以往多是依靠疲劳试验或经验来选择弹簧的喷丸强化工艺参数，这种传统作法不仅人力和物力耗费大，而且所确定的工艺参数也不一定具有最佳的强化效果。依据作者提出的并已获得试验验证的“内部疲劳极限理论”，提出一种通过工程计算获得达到预先规定疲劳强度应具备的“优化残余应力场”。这样便有可能把喷丸强化工艺技术在制造业中的应用由以往单纯依靠试验和经验而步入到以工程计算为基础与少量典型试验相结合的更科学的途径。