Identification and Functional Analysis of MicroRNAs and Their Targets in Platanus acerifolia under Lead (Pb) Stress

MicroRNAs (miRNAs) play important regulatory roles in development and stress responses in plants. Lead (Pb) is a non-essential element that is highly toxic to living organisms. Platanus acerifolia is grown as a street tree in cities throughout temperate regions for its importance in improving the urban ecological environment. MiRNAs that respond to abiotic stresses have been identified in plants; however, until now, the influence of Pb stress on P. acerifolia miRNAs has not been reported. To identify miRNAs and predict their target genes under Pb stress, two small RNA and two degradome libraries were constructed from Pb-treated and Pb-free leaves of P. acerifolia seedlings. After sequencing, 55 known miRNAs and 129 novel miRNAs were obtained, and 104 target genes for the miRNAs were identified by degradome sequencing. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to predict the functions of the targets. The expressions of eight differentially expressed miRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). This is the first report about P. acerifolia miRNAs and their target genes under Pb stress. This study has provided data for further research into molecular mechanisms involved in resistance of P. acerifolia to Pb stress.     

Synthesis of a Temperature-Sensitive Matrine-Imprinted Polymer and Its Potential Application for the Selective Extraction of Matrine from Radix Sophorae Tonkinensis

A temperature-sensitive matrine-imprinted polymer was prepared in chloroform by free-radical cross-linking copolymerization of methacrylic acid at 60 °C in the presence of ethylene glycol dimethacrylate as the cross-linker, N-isopropyl acrylamide as the temperature-responsive monomer and matrine as the template molecule. Binding experiments and Scatchard analyses revealed that two classes of binding sites were formed on molecular imprinted polymer (MIP) at 50 °C. Additionally, the thermoresponsive MIP was tested for its application as a sorbent material for the selective separation of matrine from Chinese medicinal plant radix Sophorae tonkinensis. It was shown that the thermoresponsive MIP displayed different efficiency in clean-up and enrichments using the SPE protocol at different temperatures.     

基于GIS的城市工程地质信息系统发展现状与展望

基于GIS的城市工程地质信息系统为"数字地质"的提供了重要基本数据。本文对目前基于GIS的城市工程地质信息系统的国内发展现状进行了分析与研究,总结了目前系统数据库建立过程中存在的问题以及与当前GIS技术嵌合的不足,并提出了今后发展趋势和研究方向,为城市工程地质信息系统的发展,搭建真正意义的"数字地质"提供了借鉴和参考。     

无压三产品重介旋流器的应用与研究进展

主要介绍了新型无压三产品重介旋流器的结构、原理、分类和优缺点及适用场合,并总结了该设备的应用情况和研究进展。指出该设备具有广阔的发展前景,将朝着大型化、自动化、新材料方向发展。     

十二烷基苯磺酸钠改性牡蛎壳粉对铅离子的吸附性能研究

本实验为经十二烷基苯磺酸钠改性的牡蛎壳粉对重金属铅离子吸附性能的研究,采用二甲酚橙显色剂法、紫外分光光度法测定重金属铅离子的浓度,考察了pH值、吸附时间、吸附温度、改性牡蛎壳粉添加量与铅离子初始浓度这五个因素对实验的影响.并在单因素实验的基础上,通过正交实验,结果表明,当50 mL铅离子初始浓度为100 mg/L时,吸...     

浅议高层建筑竖向测量

通过工程实例,介绍高层建筑竖向测量的原理和方法,以资借鉴。     

城市综合体建设中能效管控技术的探索与应用

探讨了城市综合体中能效管控技术的应用理念,分析了建筑中能源消耗的来源。揭示了应用能效管控技术辅助城市综合体的建设和管理,对构建城市综合体的高效运行,降低能耗具有重要意义。     

魔芋胶对南美白对虾肌原纤维蛋白凝胶特性的影响

为探究魔芋胶（konjac glucomannan,KGM）对南美白对虾肌原纤维蛋白（shrimp myofibrillar protein,SMP）凝胶性能的影响,将KGM与SMP按不同比例（1∶50、1∶20、1∶10）进行复配制备复合凝胶体系SK50、SK20、SK10,通过测定表面疏水性、内源性荧光、浊度及粒径、流变学、红外光谱、蛋白变化及凝胶微观结构,研究复合体系的凝胶特性变化。结果表明,SMP及SMP-KGM复合体系的表面疏水性、浊度及粒径随着KGM添加量的增大而增大,流变学分析表明SMP及SMP-KGM复合体系均出现剪切稀化现象,且SK20的G’值最高,其凝胶的结构稳定性最好。红外光谱分析表明SMP及SMP-KGM复合体系组的光谱特征带相似,表明无明显的基团生成。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明KGM的加入并没有引起蛋白条带的明显变化,相较于未加热组,加热后样品中肌球蛋白重链、副肌球蛋白以及肌动蛋白的条带明显减弱,说明加热能促使三者发生热聚合反应。扫描电镜与分形维数分析表明,随着KGM添加量的增加,形成了致密有序的凝胶。结果表明,一定量的KGM添加能有效提升虾糜的凝胶特性,提高虾糜类产品品质。     

40CrNiMoA钢制圆锥滚子轴承保持架兜孔加工工艺

分析圆锥滚子轴承冲压保持架的缺点,介绍了合金钢40CrNiMoA保持架材料的加工难点,改进保持架兜孔原拉削工艺为钻、铣加工,并进行了工艺对比。     

基于巡检机器人的电缆卷筒减速器故障诊断技术

针对传统电缆卷筒减速器故障诊断技术存在不同状态下的电缆卷筒减速器故障诊断误判率高的问题,提出基于巡检机器人的电缆卷筒减速器故障诊断技术。基于巡检机器人构建一个数据采集系统,利用该系统采集电缆卷筒减速器发出的振动信号数据;采用 KPCA 方法提取采集数据的特征,获得电缆卷筒减速器特征向量;将特征向量用作输入量输入到故障诊断模型中进行训练,训练期间对特征向量数据进行简化,以此提升故障诊断效率,依据输出的故障类型实现电缆卷筒减速器的故障诊断。实验结果表明,通过对该方法进行不同状态下的故障诊断误判率测试,验证了该方法的准确率高、实用性强。