Molecular Identification of the G-Protein Genes and Their Expression Profiles in Response to Nitrogen Deprivation in Brassica napus

Heterotrimeric guanine nucleotide binding protein (G-protein) consisting of Gα, Gβ, and Gγ subunits is one of the key signal transducers in plants. Recent studies indicated that G-protein has been proposed as an important mediator of nitrogen responses in rice, wheat, and Arabidopsis. However, little is known about these G-proteins in Brassica napus (B. napus), except for three identified G-proteins, BnGA1, BnGB1, and BnGG2. Therefore, the aim of the present study is to characterize the members of the G-protein gene family in allotetraploid B. napus and to analyze their expression profiles in response to nitrogen deprivation. In total, 21 G-protein family members were identified in B. napus, encoding two Gα, six Gβ, and 13 Gγ. Sequence and phylogenetic analyses showed that although genome-wide triploid events increased the number of genes encoding Gα, Gβ, and Gγ subunits, the gene structure and protein properties of the genes encoding each G-protein subunit were extremely conserved. Collinearity analysis showed that most G-protein genes in B. napus had syntenic relationships with G-protein members of Arabidopsis, Brassica rape (B. rapa), and Brassica oleracea (B. oleracea). Expression profile analysis indicated that Gα and C-type Gγ genes (except BnGG10 and BnGG12 were highly expressed in flower and ovule) were barely expressed in most organs, whereas most Gβ and A-type Gγ genes tended to be highly expressed in most organs. G-protein genes also showed various expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, Gα and five C-type Gγ genes were upregulated initially in roots, while in leaves, Gα was downregulated initially and five C-type Gγ genes were highly expressed in different times. These results provide a complex genetic dissection of G-protein genes in B. napus, and insight into the biological functions of G-protein genes in response to nitrogen deficiency.     

服务计算中避免死锁和活锁的资源分配算法

服务计算系统资源层的目标是满足服务实例的资源分配需求,保证其成功执行而不陷入死锁和活锁.首先将服务资源分配行为形式化为有限状态机;其次提出一种避免死锁和活锁的资源分配算法,它采用并发请求资源的方式,且不需要并发服务实例之间交换消息.仿真实验结果表明,该算法能够避免服务资源分配过程中的死锁和活锁,表现出较高资源分配性能.     

多传感器联合测向定位算法

本文针对传统测向定位算法的性能受限于测向精度这一问题,提出一种基于粒子滤波的多传感器联合测向定位算法。首先,推导出联合测向定位的理论定位误差,进而提出加权平均方法对初步测向定位结果进行处理得到粒子的先验分布。其次,提出测向数据融合的粒子滤波算法用于对发射源进行定位,并对该算法进行定位误差分析。理论推导和仿真结果表明本文算法能够大幅度降低定位误差,提高定位精确度,与其他测向定位算法相比体现了其先进性。      

传爆序列飞片起爆理论研究及应用

在考虑受主炸药盖片对撞击入射冲击波衰减作用的基础上，得到了改进的飞片起爆计算模型，经与实验结果对比表明这一改进模型更符合实际系统。     

CAD在爆炸网络设计中的应用

从爆炸网络的设计方法和设计手段出发,采用计算机辅助设计（CAD）实现爆炸网络的设计,提出了研究内容和实施方案。     

基于GOOSE的变电站10kV母线保护研究

文章阐述了基于GOOSE的10 kV母线保护可解决10kV母线故障问题,能满足未来数字化变电站的互操作和功能自由分布的要求。     

Discrete Element Modeling for fouled railroad ballast

Fouling refers to the condition of railroad ballast when voids in this unbound aggregate layer are filled with relatively finer materials or fouling agents commonly from the ballast aggregate breakdown, outside contamination such as coal dust from coal trains, or from subgrade soil intrusion. This paper aims to investigate the effect of fouling on ballast strength and stability. Fouling mechanism was first explained followed by investigation of mechanical properties of fouling agent. Coal dust was chosen as the fouling agent due to its poor mechanical properties and it had been reported to mostly decrease aggregate assembly strength comparing to other fouling agents. An image-aided Discrete Element Modeling (DEM) approach was introduced to simulate the coal dust fouled track field settlement performance. To that end, DEM model parameters for clean and coal dust fouled ballast were validated by matching the DEM direct shear box simulation results to the laboratory shear box testing results for both samples. By assigning laboratory calibrated model parameters to the “half-track” ballast sample generated in DEM, effects of different fouling percentage as well as different fouling locations on ballast settlement performance are studied. Results from the “half-track” DEM simulation revealed that fouling could lead to unfavorable track distresses such as “hanging tie”. Further, shoulder fouling scenario was proven to be critical in track maintenance.     

Finite Element Method Analysis of the Cutoff Wavelength of Dominant Mode in Unilateral Finline

The cutoff wavelength of dominant mode in unilateral finline is analyzed by finite element method. The cutoff wavelength can be changed by adjusting the thickness of metallic fins and dielectric constant of dielectric substrate. Numerical results provide in this paper have important values in practical applications.     

A 3D multilevel model of damage and strength of wood: Analysis of microstructural effects

A 3D hierarchical computational model of damage and strength of wood is developed. The model takes into account the four scale microstructures of wood, including the microfibril reinforced structure at nanoscale, multilayered cell walls at microscale, hexagon-shape-tube cellular structure at mesoscale and annual rings at the macroscale. With the use of the developed hierarchical model, the influence of the microstructure, including microfibril angle (MFA), the cell shape and the wood density (annual ring structure), differences between earlywood and latewood as well as microstructural arrangements and cellulose strength distributions on the tensile strength of wood is studied numerically. Good agreement of the theoretical results with experimental data has been obtained.