茶叶样品中微量元素分析方法的研究

采用HNO3-H2O2混合酸,密闭微波消解制样,ICP—AES、ICP—MS测定茶叶中P、Zn、Fe、Mn、Mg、Ca、B、Cr、Co、Ni、Cu、Mo、Cd、Pb等14种微量元素含量,建立了对植物样品中多种元素进行同时测定的方法。经国家植物标准物质验证,方法具有较好的准确性和重现性,操作流程短,检出限低,基体干扰小,RSD≤10％,加标回收率在90％～110％之间。可用于植物样品的批量分析。     

N皇后问题的启发式算法探讨

搜索策略的选择与设计是人工智能领域问题求解的核心问题之一,直接影响到问题求解过程中存储空间的占用和计算的复杂性,影响到问题求解的效率。在给出N皇后问题形式化描述和现有搜索算法的基础上,设计了3种解决N皇后问题的启发式算法,并将其与深度优先和宽度优先等搜索策略进行了分析和比较,得出了几点关于设计启发式算法的启示。     

炸药乳化器安全监测方法及其发展

文章回顾了几种现行的乳化器安全运行检测方法,分析了各自的优缺点,介绍了一种使用现代振动监测诊断技术对乳化炸药生产线的关键设备乳化器进行在线安全监测的基本原理和功能,讨论了乳化器安全监测系统的发展方向.     

Fluorescence recovery assay for the detection of protein-DNA binding

We report a novel and straightforward fluorescence recovery assay which enables the detection of protein-DNA interactions and simultaneously determines relative binding affinities of sequence-specific DNA-binding proteins for a variety of DNA sequences in a multiplexed format. The detection of protein-DNA binding is accomplished by monitoring fluorescence recovery during exonuclease digestion of DNA sequences that are modified with fluorophore-quencher pairs. Retardation of fluorescence recovery occurs with binding of the protein to the putative DNA binding element, which arrests exonuclease digestion. The assay detects protein-DNA binding in a homogeneous solution simply, quickly, and reliably without using radioisotopes. Multiplexing is possible by labeling different DNA sequences with spectrally distinct dyes, allowing simultaneous analysis of experimental and control binding reactions in the same mixture.     

Structure factors of modified liquid Al–Si alloys

The structure factor and the coordination numbers of liquid Al–Si alloys with different Si content have been measured by a high temperature X-ray diffractometer. Radial distribution functions (RDFs), the nearest atomic distance and the coordination numbers of eutectic Al–Si alloys before and after being modified with Sr and Sb were studied. The RDFs of the liquid alloy were decomposed by five Gaussian peaks. The results show that a Si–Si covalent bond exists in the liquid of eutectic and hyper-eutectic alloys. Sr in the liquid Al–Si has a capability to weaken the covalent bonds of Si–Si, suppressing the nucleation of the eutectic silicon phase. On the other hand, Sb in the liquid Al–Si increases the order degree of Si atoms, decreasing the supercooling degree of the nucleation and promoting the nucleation of eutectic silicon.     

Orbitrap高分辨质谱法快速筛查上海市流通环节蔬菜的农药残留

摘 要：目的 建立蔬菜农药多残留的筛查方法。方法 使用国家标准方法规定的QuEChERS法净化蔬菜样品,结合静电场轨道阱高分辨质谱法(orbitrap high resolution mass spectrometry)开展809种农药的快速非靶向筛查。结果 62种阳性农药的筛查限(screening detection limit, SDL)为0.005~0.010 mg/kg,定量限(limits of quantitation, LOQ)为0.01 mg/kg,线性范围为0.01~0.20 mg/kg,线性相关系数(r2)为0.9917~0.9997,日内精密度为2.77%~6.98% (n=5),日间精密度为4.82%~10.8% (n=5)。200批次样品共检出62种农药,不合格样品28批次,不合格率为14.0%。豇豆的不合格情况比较突出,不合格样品22批次,主要的不合格项是灭蝇胺和噻虫胺。结论 方法快速、准确、分析通量高,在无标准品的情况下实现了蔬菜中809种农药的非靶向快速筛查,可为蔬菜农药残留风险监测和质量控制提供支撑。     

面向学生的高校智能管理系统的设计与实现

随着网络技术的快速发展,高校对学生信息化的建设和管理愈加重视。高校智能管理系统是高校信息化建设的重要展现。本文针对学生日常的生活轨迹和生活信息,进行相关的数据分析和挖掘,采用Python语言和MySQL数据库技术,在Flask框架上进行改进,实现了以学习管理、健康管理和生活管理三大功能模块的轻量级Web和安卓应用,将面向学生的高校智能管理系统打造成一个集成化的平台,以提升校园管理的工作效率和提供健康安全保障。     

反相液相色谱法测定复方泰妙菌素注射液中泰妙菌素、磺胺嘧啶钠的含量

目的：建立反相液相色谱法测定复方泰妙菌素注射液中泰妙菌素、磺胺嘧啶钠的含量的方法。方法：采用Shim—packCLC—ODS柱（150mm×6mm,5μm）,流动相为80％乙腈-0．7％磷酸氢二铵（体积比为0．3：0．7）并用氨试液调节pH至8．0±0．1。流速为1．0mL／min,检测波长为208nm,柱温为40℃。结果：在此色谱条件下两者能完全分离,泰妙菌素在5-40μg／mL和磺胺嘧啶钠在10—80μg／mL的范围内浓度与峰面积呈良好的线性关系,回归方程和相关系数分别为：A=47205．9p＋2003．72,R=0．9999;A=77050p＋5514．75,R=0．9999。泰妙菌素和磺胺嘧啶钠的平均回8：4分别为99．99％、99．7％;RSD分别为0．70％、0．95％。结论：方法简便、快速、准确,可用作复方泰妙菌素注射液的含量测定。