MicroRNAs as Potential Biomarkers in the Differential Diagnosis of Lipomatous Tumors and Their Mimics

Adipocytic tumors are the most common subtype of soft tissue tumors. In current clinical practice, distinguishing benign lipomas from well-differentiated liposarcomas (WDLPS), as well as dedifferentiated liposarcomas (DDLPS) from their morphologic mimics, remains a significant diagnostic challenge. This is especially so when examining small biopsy samples and without the aid of additional ancillary tests. Recognizing the important role that microRNAs (miRNAs) play in tumorigenesis and their potential utility in tumor classification, we analyzed routine clinical tissue samples of benign and malignant lipomatous tumors, as well as other sarcoma mimics, to identify distinguishing miRNA-based signatures that can aid in the differential diagnosis of these entities. We discovered a 6-miRNA signature that separated lipomas from WDLPS with high confidence (AUC of 0.963), as well as a separate 6-miRNA signature that distinguished DDLPS from their more aggressive histologic mimics (AUC of 0.740). Functional enrichment analysis unveiled possible mechanistic involvement of these predictive miRNAs in adipocytic cancer-related biological processes and pathways such as PI3K/AKT/mTOR and MAPK signaling, further supporting the relevance of these miRNAs as biomarkers for adipocytic tumors. Our results demonstrate that miRNA expression profiling may potentially be used as an adjunctive tool for the diagnosis of benign and malignant adipocytic tumors. Further validation studies are warranted.     

Mutated KIT Tyrosine Kinase as a Novel Molecular Target in Acute Myeloid Leukemia

KIT is a type-III receptor tyrosine kinase that contributes to cell signaling in various cells. Since KIT is activated by overexpression or mutation and plays an important role in the development of some cancers, such as gastrointestinal stromal tumors and mast cell disease, molecular therapies targeting KIT mutations are being developed. In acute myeloid leukemia (AML), genome profiling via next-generation sequencing has shown that several genes that are mutated in patients with AML impact patients’ prognosis. Moreover, it was suggested that precision-medicine-based treatment using genomic data will improve treatment outcomes for AML patients. This paper presents (1) previous studies regarding the role of KIT mutations in AML, (2) the data in AML with KIT mutations from the HM-SCREEN-Japan-01 study, a genome profiling study for patients newly diagnosed with AML who are unsuitable for the standard first-line treatment (unfit) or have relapsed/refractory AML, and (3) new therapies targeting KIT mutations, such as tyrosine kinase inhibitors and heat shock protein 90 inhibitors. In this era when genome profiling via next-generation sequencing is becoming more common, KIT mutations are attractive novel molecular targets in AML.     

大型公路桥梁施工监理探讨

根据广深高速公路等施工实践经验,对公路桥梁水下混凝土灌注桩、主体钢筋混凝土、后张法预应力箱梁制作、桥梁架设的施工与监理过程中控制关键点进行探讨,以期加强施工与监理的工作质量。     

用于直角电桥的数字信号发生器的设计

根据直角电桥的比例臂要求,设计并实现了一种基于FPGA和DDS技术的直角电压源,所设计直角电压源可用于直角阻抗的比较,并通过实验的方法给出了作为比例臂时的相对稳定性等指标.     

基于AHM和ABC分类法的多元过程能力指数

简要介绍了多元质量特性的过程能力指数(MPCI)的研究现状,并提出基于AHM(属性层次模型)和ABC分类法的多元过程能力指数。该方法充分考虑了各个质量特性的重要性差异和不同等级的质量特性的控制目标的不同。具体介绍了该方法的步骤,并通过实例分析证明了方法的有效性和实际性。     

氯化聚乙烯/AO 2246复合材料的阻尼性能和微观形态

将氯化聚乙烯(CPE)和有机小分子2,2-亚甲基-双(4-甲基-6-叔丁基苯酚)(AO2246)混合制备了有机杂化阻尼材料,并通过动态力学分析、差示扫描量热法和扫描电子显微镜研究了阻尼材料的动态力学性能和微观结构。结果表明,在复合材料中CPE和AO 2246是相容体系;随着AO 2246含量的增加,复合材料的损耗因子(tanδ)峰值及其对应的温度逐渐增大;但当AO 2246质量分数达到50%时,复合材料的tanδ值减小而其对应的温度却继续向高温方向偏移,同时峰形变宽;复合材料中AO 2246以杂化态、微相分离态和微晶态共存,当AO 2246质量分数小于30%时,AO 2246主要以杂化态分布于基体中,复合材料中几乎没有微结晶;当AO 2246质量分数大于30%时,复合材料中AO 2246主要以微相分离态分布于基体;过量的AO 2246以微晶形式析出,形成结晶态;随着AO 2246含量的增加,复合材料中结晶数量越多。     

Materials applications of photoelectron emission microscopy

Photoelectron emission microscopy (PEEM) is a versatile technique that can image a variety of materials including metals, semiconductors and even insulators. Under favorable conditions the most advanced aberration corrected instruments have a spatial resolution approaching 2 nm. Although PEEM cannot compete with transmission or scanning electron microscopies for ultimate resolution, the technique is much gentler and has the unique advantage of imaging structure as well as electronic and magnetic states on the nanoscale. Since the image contrast is derived from spatial variations in electron photoemission intensity, PEEM is ideal for interrogating both static and dynamic electronic properties of complex nanostructured materials. Here, we review the key principles and contrast mechanisms of PEEM and briefly summarize materials applications of PEEM.     

某超高层建筑剪力墙及柱子的加固与改造设计

在某超高层建筑改造设计中,对原有结构进行了检测;根据框支柱的实际受力情况和抗震性能要求,采用了加大截面和外贴型钢相结合的方式对其进行加固,以确保延性,满足现行规范的要求。重点介绍了与基础连接以及梁柱节点处框支柱加固的处理办法,并对剪力墙上增设洞口、新旧剪力墙的连接以及边缘构件的处理方式进行了介绍,可为类似项目的结构加固设计提供一定的参考。     

A physics-based simulation approach for cooperative erection activities

Cooperative erection activities are critical to projects which involve the erection of heavy loads or the installation of special equipment. Detailed simulation on computer prior to construction can identify constructability problems, and subsequently avoided during actual erections. This paper describes an integrated approach for simulating the detailed motions of cranes. This research develops a physics-based model that follows the principle of closed-form forward kinematics and constraint-based dynamics to present the dual-crane mechanism mathematically — a non-trivial task. This model can be used to analyze the inputs from the users (i.e. virtual crane operators) and simultaneously compute the cables sway and reaction of collisions. We also implemented the model on computer and developed a simulation system, Erection Director, to render realistic cooperative erection activities. A demonstration of simulating two-crane lift has been built and three performance tests including a small building (840 elements), a medium building (1937 elements) and a large building (2682 elements) validate the feasibility of the proposed approach. The test results indicate that Erection Director can support real-time and physics-based visualization of cooperative erections.