Microscale epitope mapping by affinity capillary electrophoresis-mass spectrometry

Using beta-endorphin as a model system, a new microscale solution-based approach for linear epitope mapping based on affinity capillary electrophoresis-mass spectrometry (ACE-MS) is demonstrated. Tryptic peptides are separated in a neutral coated capillary and monitored by ultraviolet absorbance and electrospray mass spectrometry. Then, following injection of the peptide digest mixture, anti-beta-endorphin antibody is injected. The peptide, which binds to the antibody, is captured and disappears from its migration time. Following this subtraction-screening procedure, the binding of the individually synthesized or isolated immunoreactive peptide is examined by the ACE-MS procedure to confirm that the epitope resides on the peptide. A series of truncated peptides can then be made and the precise epitope determined by ACE-MS. The method utilizes low femtomole amounts of antibody and peptide digest per run and is rapid and easily automatable.     

Simultaneous phenytoin and warfarin toxicity on chronic concomitant therapy

Pneumoperitoneum in diagnostic or therapeutic laparoscopy can be achieved by an open or a closed technique. The authors, on the basis of their experience, compare both techniques, concluding that open method is safer, because of the lack of major complications observed in the closed method and with only few minor complications.     

PHC预应力管桩在我省高速公路桥梁基础上的首次应用

PHC预应力管桩具有混凝土强度高、单桩竖向承载力高、施工进度快等优点,目前在我国高速铁路、高速公路的桥梁桥涵及软地基处理中得到广泛采用。文章主要介绍了PHC预应力管桩在安徽省高速公路桥梁桥墩基础中应用的一个具体工程案例,经过实践证明,PHC预应力管桩在立交桥桥墩基础中具有很好的应用效果。