Fluorescence Anisotropy of Cellular NADH as a Tool to Study Different Metabolic Properties of Human Melanocytes and Melanoma Cells

In this study, we wanted to see if fluorescence anisotropy could be used to detect changes in metabolism in cells with significant light scattering and absorption properties. Fluorescence anisotropy measurements of nicotinamide adenine dinucleotide (NADH) were performed with human melanocytes and melanoma cell lines. To demonstrate the feasibility of using fluorescence anisotropy for detecting metabolic changes, the electron transport chain was blocked using rotenone, inducing an accumulation of intracellular NADH. Total fluorescence increased in all cells as a result of rotenone treatment. Fluorescence anisotropy decreased in the rotenone-treated cells relative to the controls, suggesting an increased ratio of free to protein-bound NADH in the treated cells. In general, the fluorescence anisotropy of the melanocytes was significantly higher than that of the melanoma cell lines. Reflectance spectroscopy showed that the differences in fluorescence anisotropy between the cell types were not due to differences in scattering and absorption properties. Intrinsic cellular NADH fluorescence was experimentally extracted by ratioing polarized fluorescence to polarized reflectance. NADH binding, measured as the ratio of fluorescence intensity at 430 and 465 nm, showed more protein-bound NADH in the melanocytes than in the melanoma cells, consistent with the fluorescence anisotropy measurements.     

皂河闸小水电存在问题及改造探讨

针对皂河闸水电站因长期运行造成的机电设备老化、机组效率较低等问题,对3号机进行技术改造。经过调研分析,提出改造方案,并对改造前后的经济效益进行对比分析。表3个。     

水利产业化研究

运用经济管理理论,论述了水利产业的特征和水利产业的类属;从水利产业的性质和地位论述水利作为基础产业的属性;阐明了水利产业化的概念和目标。提出水利应建立产业化的指标体系,并走产业化发展的路子。     

Silicon power MOSFET at low temperatures: A two-dimensional computer simulation study

Understanding how the structure of the unit-cell affects the cryogenic performance of a Si power Metal Oxide Semiconductor Field Effect Transistor (MOSFET) is an important step toward optimizing of the device for cryogenic operations. In this paper, numerical simulations of the Si power Double Diffused MOSFET’ (DMOS) are performed at room temperature and cryogenic temperatures. Physically based models for temperature dependent silicon properties are employed in the simulations. The performances of power DMOS’ with various unit-cell structures are compared at both room temperature and low temperatures. The effect of the cell structure on the on-resistance and breakdown voltage of the device are analyzed. The simulation results suggest that the device optimized for room temperature operation can be further optimized at cryogenic temperatures.     

Direct evaluation of singular integrals in elastoplastic analysis by the boundary element method

This paper presents a new method for the direct and accurate evaluation of strongly singular integrals in the sense of Cauchy principal values and weakly singular integrals over quadratic boundary elements in three-dimensional stress analysis and quadratic internal cells in two-dimensional elastoplastic analysis by the boundary element method. A quadratic triangle polar co-ordinate transformation technique is applied to reduce the order of singularity of the singular integrals. Next, a form of Stokes' theorem is introduced in order to remove the singularity in the Cauchy principal value integrals; therefore, the evaluation of these integrals can be carried out by standard Gaussian quadrature. Numerical examples of 2-D elastoplastic problems and a 3-D elastic problem show the effectiveness and efficiency of the method.     

β放射性气体浓度计的新分度方法

本文介绍β放射性气体浓度计的一种新的分度方法——泄漏元件排气法。其特点是一只元件可分度放射性气体浓度计的许多读出数(甚至是几个量程),操作方便。     

Improved coupled-mode theory of anisotropic optical waveguides

A coupled-mode equation for anisotropic waveguide systems of arbitrary cross section and general dielectric distribution is derived. Numerical results comparing the exact calculations to those of the method of Hardy et al. (Opt. Lett., vol.11, 742-4, 1986) show that the same accuracy can be obtained not only for TE, but also for TM mode coupling in the case of anisotropic waveguides, and the improved coupled-mode theory is applicable to the situation when moderately strong coupling occurs under the condition where the edge-to-edge separation of two coupled guides D₂ is about 0.1 μm     

Iterative receivers for space-time block-coded OFDM systems indispersive fading channels

We consider the design of iterative receivers for space-time block-coded orthogonal frequency-division multiplexing (STBC-OFDM) systems in unknown wireless dispersive fading channels, with or without outer channel coding. First, we propose a maximum-likelihood (ML) receiver for STBC-OFDM systems based on the expectation-maximization (EM) algorithm. By assuming that the fading processes remain constant over the duration of one STBC code word and by exploiting the orthogonality property of the STBC as well as the OFDM modulation, we show that the EM-based receiver has a very low computational complexity and that the initialization of the EM receiver is based on the linear minimum mean square error (MMSE) channel estimate for both the pilot and the data transmission. Since the actual fading processes may vary within one STBC code word, we also analyze the effect of a modeling mismatch on the receiver performance and show both analytically and through simulations that the performance degradation due to such a mismatch is negligible for practical Doppler frequencies. We further propose a turbo receiver based on the maximum a posteriori-EM algorithm for STBC-OFDM systems with outer channel coding. Compared with the previous noniterative receiver employing a decision-directed linear channel estimator, the iterative receivers proposed here significantly improve the receiver performance and can approach the ML performance in typical wireless channels with very fast fading, at a reasonable computational complexity well suited for real-time implementations     

一种简易可靠的射频激光调制器

采用ＰＩＮ电调衰减器、ＴＬ４９４等元件组成了射频（ＲＦ）大功率ＣＯ２激光调制器，调制频率、占空比和调制度单独可调。给出了电路的工作原理和测试结果。