鲜红斑痣光动力治疗的模型仿真初步研究

为了研究光敏剂特性对光动力疗法(PDT)治疗鲜红斑痣(PWS)疗效的影响，帮助临床采取有效的治疗方案，建立了光动力治疗鲜红斑痣的数学模型。建立了卟啉类光敏剂受光激发产生单线态氧的数学模型以及光敏剂自身漂白过程和组织中扩散过程的数学模型，以国产光敏剂血啉甲醚(HMME)的实验数据为例，用蒙特卡罗方法仿真组织中的光分布，应用建立的单线态氧产生过程的数学模型，仿真光动力治疗鲜红斑痣过程中，组织内单线态氧产量的分布。对比不同的光敏剂漂白速度对组织中单线态氧产量的影响，发现漂白速率越快，破坏血管的同时对表皮和真皮组织的保护作用越好。这些结果为光动力疗法治疗鲜红斑痣的临床应用提供了具有针对性的指导。     

先天性动静脉瘘的腔内治疗

目的探讨微创腔内治疗先天性动静脉瘘的有效性、安全性。方法回顾性分析我院2006年3月-2009年10月30例先天性动静脉瘘的临床资料,完善相关检查后行血管造影检查及腔内治疗术,术后每3个月进行随访。结果25例经腔内治疗后成功封堵或栓塞动静脉瘘,症状改善,无并发症和围手术期死亡。5例因术中无法配合、风险过大、血管过细微导管无法通过等原因未行治疗。平均随访6个月。随访期内5例症状复发,但较腔内治疗前轻。结论腔内治疗先天性动静脉瘘疗效明确、安全、微创,短期随访效果满意。     

Printing-Induced Alignment Network Design of Polymer Matrix for Stretchable Perovskite Solar Cells with Over 20% Efficiency

Polymer matrix is felicitously applied into the active layer and transporting layer of perovskite solar cells (PSCs) to enable a stretchable function. However, the chaotic deposition of polymer chains is the main cause for the inferior photoelectric performance. When the stretchable PSCs are in a working state, the stress cannot be removed effectively due to the random polymer chain deposition. The stress accumulation will cause irreversible damage to the stretchable PSCs. Herein, the structural bionics and patterned-meniscus coating technology are combined to print the polymer chain-oriented deposition in the stretchable PSCs. Based on this approach, the conducting polymer electrode is printed with both significant mechanical stability and conductivity. More importantly, the oriented polyurethane with self-healing property can enhance the crystal quality of perovskite films and repair perovskite cracks caused by stress destruction. Thus, the corresponding stretchable PSCs achieve a stabilized power conversion efficiency (PCE) of 20.04% (1.0 cm²) and 16.47% (9 cm²) with minor efficiency discrepancy. Notably, the stretchable PSCs can maintain 86% of the primitive PCE after 1000 cycles of bending with a stretch ratio of 30%. This directional growth of polymer chain strategy provides guidance for printing prominent-performance stretchable PSCs.     

ANGIO Mentor血管介入手术模拟器在进修医师教学中的应用

【摘要】 目的 探讨ANGIO Mentor血管介入手术模拟器在介入放射科进修医生教学中的应用价值。方法 将无介入手术经验的进修医师分为A组51名（接受传统模式培训）和B组38名（在进行传统模式培训前先接受模拟器基础培训1个月）。比较两组实际介入操作熟练程度、介入操作时间、失误发生等情况,判断培训效果。结果 B组实际介入操作熟练程度、介入操作时间、介入操作失误等明显优于A组。结论 该模拟器有助于全面提高介入放射科进修医师介入操作能力。
     

云南栽培玛咖芥子油苷提取工艺的优化

研究了云南栽培玛咖中芥子油苷的提取工艺。采用甲醇为溶剂,在单因素试验的基础上设计正交试验,得到云南栽培玛咖中芥子油苷的最优提取条件,并对超声辅助提取工艺进行研究。结果显示:水浴浸提最优提取条件为,提取温度40℃,料液比1∶50(g∶mL),提取12 h,在此条件下芥子油苷提取率为1.45%。采用超声辅助提取法,在室温下提取20 min,提取率为1.66%。超声辅助提取是一种有效的玛咖芥子油苷提取工艺。     

浅低温不停跳心内直视手术体外循环436例分析

目的分析浅低温不停跳下心内直视手术体外循环病例选择及临床意义。方法对1999年3月-2009年5月期间436例简单先心浅低温不停跳下心内直视手术体外循环灌注情况进行回顾性研究。年龄≤12岁小儿组263例,年龄>12岁成人组173例,对两组患者体外循环中流量、压力、温度等情况进行比较。结果与成人比较,小儿灌注流量明显高于成人(P<0.01),平均动脉压明显低于成人(P<0.01);术中平均温度和转机时间两组无统计学差异(P>0.05)。436例患者术后均痊愈出院。结论体外循环下心脏不停跳心内直视手术,对于简单先心病患者是安全可靠的。     

有效减少老年PCI术后穿刺部位出血量的护理

目的探讨老年PCI术后并发穿刺部位出血的观察要点及护理措施。方法将122例行PCI术的患者按手术的先后顺序随机分为实验组及对照组,实验组患者术后根据详细护理路径及决策系统进行护理,对照组按PCI术后护理常规进行护理,比较两组患者术后穿刺部位出血量的多少及预后。结果实验组患者穿刺部位出血量少于对照组(P<0.05)。结论按照预先设计的老年PCI术后穿刺部位出血量观察表进行病情观察,并采取相应护理措施,可有效防止穿刺部位过多出血及意外的发生。     

全反式维甲酸对恶性黑素瘤A375细胞VEGF表达的影响

目的研究全反式维甲酸(ATRA)对体外培养恶性黑素瘤A375细胞VEGF表达影响。方法应用Real-time PCR检测A375细胞VEGF-B在基因水平的表达。应用ELISA法检测A375细胞上清中VEGF蛋白水平的表达。结果ATRA可降低A375细胞培养上清的VEGF的mRNA表达和血清浓度,与对照组相比有统计学意义(P<0.05)。结论ATRA可在mRNA和蛋白水平下调A375细胞VEGF的表达,且这种抑制作用呈时间剂量依赖关系。     

Cobweb-Inspired Microenvironment-Targeting Nanosystem with Sequential Multiple-Stage Stimulus-Response Capacity for Ischaemic Tissue Repair

Myocardial ischaemia is pathologically complicated; various changes in intracellular and extracellular microenvironments make it essential to develop a smart drug system with multiple stimulus responses to adapt to the complex process. Inspired by the cobweb, this study designs a microreticular nanosystem that adheres to tissue and is sequentially responsive to multiple stimuli in the ischaemic microenvironment. The nanosystem is fabricated from hyaluronic acid (HA), ROS-responsive B-PDEA, and hypoxia-sensitive VEGF-expressing plasmids (EPODNA) through electrostatic interactions. After intramyocardial injection, the tissue-adhesive property of the nanosystem will significantly decrease its acute loss from the injection site. Extracellularly, the microreticular nanosystem first responds to activated hyaluronidase (hyal), releasing HA for microenvironment regulation and B-PDEA/DNA nanoparticles (NP) with high transfection efficiency for cardiac cells. Intracellularly, ROS sequentially induced B-PDEA/DNA NP dissociation, consuming some ROS to attenuate oxidative stress and releasing DNA to promote its expression. Meanwhile, local hypoxia significantly activates VEGF expression in plasmids for myocardial revascularization and repair. The function of the microreticular nanosystem is systematically evaluated in vitro. In a rat model of myocardial infarction, treatment with the microreticular nanosystem significantly promotes functional and structural improvements. Collectively, the study provides a promising smart nanosystem to promote tissue repair after complex damage.