Immobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers

Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Cross-linking of amino groups of enzyme with 5% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3% of activity yield while percent retention of PPO activity was found to be 83.5%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability.     

Mapping streaming applications on multiprocessors with time-division-multiplexed network-on-chip

This paper addresses mapping of streaming applications (such as MPEG) on multiprocessor platforms with time-division-multiplexed network-on-chip. In particular, we solve processor selection, path selection and router configuration problems. Given the complexity of these problems, state of the art approaches in this area largely rely on greedy heuristics, which do not guarantee optimality. Our approach is based on a constraint programming formulation that merges a number of steps, usually tackled in sequence in classic approaches. Thus, our method has the potential of finding optimal solutions with respect to resource usage under throughput constraints. The experimental evaluation presented in here shows that our approach is capable of exploring a range of solutions while giving the designer the opportunity to emphasize the importance of various design metrics.     

Hierarchical representation of video sequences for annotation

Video annotation is an important issue in video content management systems. Rapid growth of the digital video data has created a need for efficient and reasonable mechanisms that can ease the annotation process. In this paper, we propose a novel hierarchical clustering based system for video annotation. The proposed system generates a top-down hierarchy of the video streams using hierarchical k-means clustering. A tree-based structure is produced by dividing the video recursively into sub-groups, each of which consists of similar content. Based on the visual features, each node of the tree is partitioned into its children using k-means clustering. Each sub-group is then represented by its key frame, which is selected as the closest frame to the centroids of the corresponding cluster, and then can be displayed at the higher level of the hierarchy. The experiments show that very good hierarchical view of the video sequences can be created for annotation in terms of efficiency.     

Mixed monolayer and mixed micelle formation in binary mixture of surfactants—Systems for trioxyethylenated dodecyl sulfonate and some polyoxyethylenated fatty alcohol ether nonionic surfactants

The interaction and synergism of some polyoxyethylenated fatty alcohol ether (POE) nonionic surfactants (C₁₂E₂, C₁₂E₃, C₁₀E₅, C₁₀E₇, where C_x indicates number of carbon atoms in the chain and E_y indicates number of oxyethylene glycol ethers) with trioxyethylenated dodecyl sulfonate (C₁₂E₃S) in mixed monolayer formation at the surface and in mixed micelle formation in aqueous solutions were studied at 25 and 40°C by calculating interaction parameters (β_α, β_M) from surface tension-concentration data by use of Rosen's equations based on the nonideal solution theory. All the systems investigated adapt reasonably well to the nonideal model, with negative values of β_σ and β_M (where M means micelle and σ refers to the air-liquid interface) indicating a favorable interaction between the mixed surfactants. Either at a monolayer or in a mixed micelle, the attractive interaction becomes stronger when the alkyl chain in the POE surfactant is longer, i.e., when the POE becomes more hydrophobic. The interaction increases in the order C₁₀E₇<C₁₀E₅<C₁₂E₃, C₁₂E₂. For the two C₁₀E _n (n= 5,7)/C₁₂E₃S systems, as temperature increases from 25 to 40°C, the interaction increases in a mixed micelle, but it decreases in a mixed monolayer. Synergism in mixed micelle formation exists for C₁₂E₃S/C₁₀E _n mixtures when X₁ ^M , the mole fraction of POE in a mixed micelle, is ≈0.4–0.8, whereas synergism does not occur in the systems of C₁₂E₃S/C₁₂E _m due to the large difference between CMC₁ and CMC₂, i.e., large |In(C ₁ ^M /C ₂ ^M )| value (where CMC=critical micelle concentration). The degree of synergism in mixed micelle formation is temperature independent and is 0.23, 0.18, and close to zero for C₁₀E₅/C₁₂E₃S, C₁₀E₇/C₁₂E₃S, and C₁₂E _m (m=2,3)/C₁₂E₃S systems, respectively. Synergism in surface tension reduction effectiveness occurs in C₁₂E₃S/C₁₂E₂ and C₁₂E₃S/C₁₂E₃ systems. The mole fractions of POE in the solution phase are 0.302 and 0.333 for the two mixtures at the point of maximum synergism.     

大规模认知无线电网络的时延分析

时延作为无线网络的最基本的性能之一,对网络信息分发、路由协议设计、节点部署等都具有重要意义。与传统的无线网络不同,认知无线电网络的频谱资源具有动态变化性,该特性会对网络时延产生极大的影响。因此,如何对动态频谱环境下的大规模认知无线电网络进行时延分析,是一项很具挑战性的课题。为此,首先对动态频谱环境进行建模,将认知用户的频谱接入过程建模为一个连续时间的马尔可夫链,并建立认知用户的生存函数来量化授权用户活动以及信道数量对频谱环境的影响;其次,将上述模型与首次通过渗流理论结合起来,研究了大规模认知无线电网络时延的伸缩规律,并获取了更为精确的时延与距离比的上限值。理论分析及仿真结果表明,动态频谱环境与密度一样会对时延产生极大影响。研究结论对认知无线电网络的设计具有重要的指导意义。     

环-皮特森簇网格的资源搜索机制

设计了一种以环 皮特森图(RP(k))结构为骨干拓扑,以簇为资源节点的文件资源系统环 皮特森簇网格(RP(k)C grid),讨论了RP(k)C grid系统的消息路由算法. 提出了簇头探察、簇头泛洪和环 皮特森簇(RP(k)C)轮询相结合的probe flooding and scan搜索机制. 对成功跨簇查询的文件在RP(k)C管理节点上建立副本,通过查询登记机制避免簇内泛洪. 实验结果表明,RP(k)C grid有效降低了网络负载和查询延迟,具有良好的可扩展性.     

Development of an index based on fish,macroinvertebrates, phytoplankton,and physicochemical properties to assess urban aquatic ecosystems in Jinan,China

Because of the rapid growth in urbanization over recent decades, urban aquatic ecosystems have been subjected to unprecedented threats and challenges. In this study, we visited 48 sites, of which 14 were reservoir sites, in water bodies in Jinan, China, on six occasions in May, August, and October of 2014 and 2015. We sampled fish, macroinvertebrates, and phytoplankton assemblages and physicochemical factors at each site. We used the richness and diversity of fish, macroinvertebrates, and phytoplankton to evaluate the condition of the aquatic ecosystems of the river sites. We also evaluated the water quality of the reservoir sites using the physicochemical factors of the State Environmental Protection Administration of China. We classified the results into five levels—excellent, good, moderate, poor, and bad—with the comprehensive evaluation index (CEI). More than 40% of the sampling sites were in either poor or bad condition. Nearly half of the excellent or good sites were in the south of Jinan. The CEI was significantly different between the reference and impaired sites; it was significantly and positively correlated with the qualitative habitat evaluation index and was significantly and negatively correlated with conductivity, total alkalinity, and ammonia nitrogen.     

Supervised discrete hashing through similarity learning

Multimedia Tools and Applications - Supervised hashing has achieved better accuracy than unsupervised hashing in many practical applications owing to its use of semantic label information. However,...