PURIFICATION AND PROPERTIES OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn²⁺ and NEM, and not affected obviously by Ba²⁺, Ca²⁺, Co²⁺, Cu²⁺, Fe²⁺, Fe³⁺, Mg²⁺, Mn²⁺, Na⁺ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases.     

基于FPGA乘法器架构的RNS与有符号二进制量转换

RNS(余数数制系统)是一种整数运算系统,在粒度精确性,能源损耗和响应速度上有很大的优势.从RNS到二进制数的输入输出转换是基于余数算法的专用架构实现的关键.本文提出了一个基于N类模的RNS与有符号二进制量的通用转换算法在FPGAs的乘法器上的实现过程.该算法能更有效地进行有符号数与RNS的转换.基于该算法类型乘法器在同类型乘法器中显示出了速度优势.文章中该架构被映射到Altera的10K系列的FPGA上.     

Calculations of optical properties in p-doped nitrides quaternary alloys multiple quantum wells

We present theoretical photoluminescence (PL) and absorption spectra of p-doped InGaN/AlInGaN and AlInGaN/InGaN multiple quantum wells (MQWs). The calculations were performed within the k.p method by means of solving a full eight-band Kane Hamiltonian together with the Poisson equation in a plane wave representation, including exchange–correlation effects within the local density approximation. Strain effects due to the lattice mismatch and an internal electric field are also taken into account. We show that by changing the In and Al composition we can reach short and long emission wavelengths. The trends in the calculated Stokes shift, due to many-body effects within the quasi-two-dimensional hole gas (2DHG), are analyzed as a function of the acceptor doping concentration. Since the studies of optical properties of quantum wells based on nitrides quaternary alloys are at an early stage, the results reported here will provide guidelines for the interpretation of forthcoming experiments.     

Alleviation Effect of Lanthanum on Cadmium Stress in Seedling Hydroponic Culture of Kidney Bean and Corn

Since1970s,rare earths(RE)have been exten-sively used as micro-fertilizers for crops in China.Ithas already been proved that REcani mprove the pro-duction and quality of crops.However,there havebeen a fewinvestigations about using RE as regulatorin pollution ecology.For example,An et al[1]studiedthe effects of O3on wheat growth and the protectiveeffect of RE.Yan and Zhou et al[2,3]discussed theprotective effect of RE on plants under acid rainstress.Jia et al[4]and Hu et al[5]reported th…     

COMPARATIVE STUDY OF CHEMICAL COMPOSITION AND PHYSICOCHEMICAL PROPERTIES OF DEFATTED WHEAT GERM FLOUR AND ITS PROTEIN ISOLATE

Chemical composition and physicochemical properties of defatted wheat germ (WG) flour (DWGF) and protein isolate (DWGPI) were evaluated to determine their potential as food ingredients. Protein content of DWGF was 31.42% while that of DWGPI was 84.18%. Comparison of the amino acid composition to the Food and Agriculture Organization/World Health Organization reference pattern indicated that DWGF and DWGPI had well‐balanced amino acid profiles. Both of them were relatively rich in lysine, whereas cystine was lacking. The main mineral constituents of DWGF were potassium, magnesium and calcium, while sodium was the dominating element in DWGPI as a result of salt extraction. Concentrations of potassium, magnesium, calcium, manganese and zinc in DWGPI were significantly decreased because of alkaline extraction compared to those in DWGF. The elements copper and iron were higher in DWGPI than in DWGF. Total flavonoid content of DWGF was about 0.35 g rutin equiv/100 g, whereas that of DWGPI was lower (about 0.24 g rutin equiv/100 g). Compared to soybean protein isolate, DWGPI was easily digested by pepsin in vitro, while DWGF was comparatively indigestible. Nonreduced and reduced sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analyses seem to show that interpolypeptide S‐S bonds were absent in the structure of WG proteins. DWGF and DWGPI shared four groups of bands: (1) 85–70 kDa; (2) 55–50 kDa; (3) 47–40 kDa; and (4) below 20 kDa.     

QPSO算法在非线性观测器设计中的应用*

具有量子行为的粒子群优化(Quantum-behaved Particle Swarm Optimization,QPSO)算法是继粒子群优化算法(Particle Swarm Optimization,PSO)后,最新提出的一种新型、高效的进化算法.提出了运用QPSO算法设计的非线性观测器方法.该方法属于滚动时域估计方法,利用具有量子行为的粒子群算法优化获得系统状态的最优估计.仿真结果显示该方法对初始条件不敏感,具有很强的跟踪能力.     

《无机及分析化学》在多媒体教学的应用及思考

对多媒体技术在无机及分析化学教学的应用进行了，阐述和分析，指出使用多媒体教学应注意的问题。     

Preparations and properties of thermosensitive terpolymers of N‐isopropylacrylamide,sodium 2‐acrylamido‐2‐methyl‐propanesuphonate,and N‐tert‐butylacrylamide

Terpolymers based on N‐isopropylacrylamide, sodium 2‐acrylamido‐2‐methyl‐propanesulfonate, and N‐tert‐butylacrylamide were synthesized by free‐radical copolymerization with 2,2′‐azobisisobutyronitrile as an initiator. The lower critical solution temperatures (LCSTs) of the linear polymer aqueous solutions were determined by the measurement of the transmittance on UV at different temperatures. The influence of the polymer concentration, polymer composition, and ionic strength on the LCSTs of the linear polymers was investigated. The LCST decreased with increases in the hydrophobic monomer N‐tert‐butylacrylamide, polymer concentration, and ionic strength. The phase transition became sharp when the polymer concentration and ionic strength increased. Meanwhile, the crosslinked hydrogels were prepared with the same recipe used for the linear terpolymers, but a crosslinker was added to the reaction system. The swelling ratios of the hydrogels at various temperatures and salt solutions were determined. The hydrogels possessed both high swelling ratios and thermosensitivity. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007     

微波真空干燥高粘度的灵芝浓缩液

热敏性、高粘度物料的干燥仍是现代干燥技术中的一个难题。灵芝水提后，由薄膜蒸发器浓缩至含水量70％左右，然后采用微波真空干燥（真空度3000Pa）至水分含量10％左右，改用传统的电热真空干燥（55～60℃）至含水量6％～7％。对干燥产品的主要生物活性成分——灵芝多糖和三萜酸进行了分析检测并与其它干燥方法进行了比较。结果表明：利用微波真空干燥的灵芝产品其灵芝多糖和三萜酸的保留率与冷冻干燥产品十分接近。而比传统真空干燥（60—65℃）的产品要高得多，此外采用微波真空干燥的干燥时间要短得多。     

DTPA-双(3,5-二甲氧基-4''''-氨基二苯乙烯)的合成

以3,5-二甲氧基苄溴为起始原料,通过Witting-Hornor反应,再经Fe/HCl还原,合成了3,5-二甲氧基-4'-氨基二苯乙烯.再与DTPAA反应得到标题化合物,其结构经IR、MS、1HNMR、元素分析确证.