云南省烟草正番茄斑萎病毒属病毒的调查和鉴定

  背景和目的  烟草是云南省支柱产业,近年来烟草斑萎类病害发生严重。了解烟草斑萎类病害在云南的病原种类、病害发生、流行和分布势在必行。  方法  2012以来,本课题组从云南省10个地州市共采集疑似Orthotospovirus属病毒侵染的烟草样品38份,采用症状学观察、酶联免疫吸附反应以及RT-PCR鉴定。  结果  结果显示有20份烟草样品感染Orthotospovirus属病毒,其中番茄斑萎病毒单独侵染样品11份、朱顶红褪绿环斑病毒单独侵染样品4份、番茄环纹斑点病毒单独侵染样品2份、马蹄莲褪绿斑病毒单独侵染样品2份、番茄斑萎病毒和朱顶红褪绿环斑病毒复合侵染1份。  结论  本研究首次报道朱顶红褪绿环斑病毒侵染烟草,同时发现番茄斑萎病毒和朱顶红褪绿环斑病毒复合侵染烟草,鉴定结果对于科学制定烟草斑萎病害综合防治措施,保障云南省烟草种植业的持续健康发展具有重要意义。      

毛细管气相色谱法同时测定异丙甲草胺和莠去津

采用毛细管气相色谱法,在R tx-1701(30 m×0.32 mm×0.25μm)毛细管柱上以三唑酮为内标,氢火焰离子化检测器(FID)检测对异丙甲草胺和莠去津混剂,标准偏差分别为0.23%、0.20%,变异系数分别为0.919%、0.995%,线性相关系数分别为0.9987、0.9992,平均回收率为:99.7%、99.6%。     

Fast aging treatment for the synthesis of hydrocalumites using microwaves

Friedel's salt [Ca₂Al(OH)₆]Cl·2H₂O, commonly known as hydrocalumite, was synthesized by co-precipitation following different aging treatments, using microwave irradiation or conventional heating and by refluxing or in autoclave, with the aim of decreasing the aging times (24 h) employed when aging by conventional heating or at room temperature. Hydrocalumites were characterized by XRPD, FT-IR, N₂ physisorption, ICP-OES, TEM, TEM-electron diffraction and TGA techniques. The use of microwaves favors a faster crystalline growth in the stacking direction whereas the use of autoclave improves the lamellar crystallinity. The highest crystallinity was found for the sample aged with microwave irradiation by autoclaving at 453 K for 1 h. This sample presented the highest crystallite size in the stacking direction (76 nm), the largest lamellar crystals (4000 nm) with R3c symmetry, and the highest dehydration- and dehydroxylation temperatures.     

The spectral characteristics of dissolved organic matter from sediments in Lake Baiyangdian,North China

Dissolved organic matter (DOM) extracted from sediment samples in Lake Baiyangdian was investigated using UV -Vis absorption and fluorescence spectroscopy. Two parameters, the ratios of absorbance and dissolved organic carbon at 254° nm (SUVA₂₅₄) and the integral area from 240° nm to 400° nm (A_{240 -400}), were used to assess the molecular weight and aromaticity of DOM. The surface sediments showed a relatively low aromaticity of DOM due to the impact of environmental pollution. Synchronous fluorescence spectra showed an extremely high fraction of the protein-like region (PLR). Parallel factor analysis (PARAFAC) of excitation -emission matrix spectra revealed four fluorescent components occurring in sediment DOM: two humic-like (C1 and C2) components and two protein-like (C3 and C4) components. The PARAFAC-PCA displayed three PCA factors (factors 1, 2 and 3) which accounted for 48.250%, 25.927% and 25.391%, respectively, of the variance in fluorescent component. The humic-like components 1 and 2 concurrently showed positive factor 1 loadings. Factor 2 was mainly explained by the tryptophan-like component C3. A non-humic component 4, having a low factor loading in the other two factors, showed an extremely high factor 3 loading. The EEM -PARAFAC-PCA showed varying contributions of terrestrial versus autochthonous DOM sources in lake sediments.     

基于GIS的云南省土壤环境质量数据管理系统

为了揭示云南省1718个采样点的土壤环境质量统计、评价与对比分析结果数据之间的空间关系和空间分布模式,设计和实现了云南省土壤环境质量数据管理系统,该系统采用GIS进行基于GIS组件的二次开发,以C#作为开发语言,SQLServer作为数据库,来实现信息的输入、维护、查询、分析、专题制图、管理等基本功能,以空间可视化的方法实现了空间信息和属性信息的集成管理,并完善地建立了二者之间的联系。     

昆交会与南博会的比较研究及发展建议

作为与东盟国家合作的前沿省区,云南和广西存在着合作与竞争的双重关系,这种关系集中表现在昆交会和南博会上.两会不仅是云南和广西与东盟国家经贸合作的代表性展会,也是中国开展与东南亚、南亚国家经济合作的重要渠道.通过对昆交会与南博会的发展背景、会展特点、举办情况、前景等各方面的对比,提出展会能够结合自身区域特点向综合性博览会发展.     

药品包装材料和容器炽灼残渣测量不确定度评定

炽灼残渣是衡量一些药品包装材料和容器品质的重要指标,同时测量不确定度是评价结果的重要手段,本文以口服固体药用高密度聚乙烯瓶、聚丙烯输液瓶、注射液用卤化丁基橡胶塞3种具代表性的产品为例,以建立炽灼残渣测量不确定度的评定方法。依据YBB00122002等标准的要求对各产品的炽灼残渣进行检测并按照JJF1059的要求对其炽灼残渣测量结果不确定度进行分析评定。以口服固体药用高密度聚乙烯瓶为例,其炽灼残渣测量结果可以表示为皓（2．0±0．14）％,k=2,此测量不确定度评定方法具有实用价值。     

义齿的金属-陶瓷结合特性测量不确定度评定

目的 :建立义齿金属-陶瓷结合特性测定方法的不确定度评定方法。方法 :依据标准YY0621-2008测定义齿中金属-陶瓷结合特性的值,依据JF1059.1-2012确定测量不确定度的分量,合成不确定度。结果 :当样品的分离/断裂力起始强度为47 MPa时,合成不确定为1.7MPa,扩展不确定度为3.4MPa。不确定度报告表示为:τb=47 MPa,Uτb=3.4 MPa,k=2。结论:从金属基体厚度的测量、k值的读数以及万能材料试验机等方面对引起不确定性的因素进行详细讨论和计算,指出影响金属陶瓷结合特性准确性的主要因素是金属基体厚度测量误差和k值读数误差。     

Intercomparison of Humidity and Temperature Sensors:GTS1, Vaisala RS80, and CFH

GTS1 digital radiosonde, developed by the Shanghai Changwang Meteorological Science and Technology Company in 1998, is now widely used in operational radiosonde stations in China. A preliminary comparison of simultaneous humidity measurements by the GTS1 radiosonde, the Vaisala RS80 radiosonde, and the Cryogenic Frostpoint Hygrometer (CFH), launched at Kunming in August 2009, reveals a large dry bias produced by the GTS1 humidity sensor. The average relative dry bias is in the order of 10% below 500 hPa,increasing rapidly to 30% above 500 hPa, and up to 55% at 310 hPa. A much larger dry bias is observed in the daytime, and this daytime effect increases with altitude. The GTS1 radiosonde fails to respond to humidity changes in the upper troposphere, and sometimes even in the middle troposphere. The failure of GTS1 in the middle and upper troposphere will result in significant artificial humidity shifts in radiosonde climate records at stations in China where a transition from mechanical to digital radiosondes has occurred.A comparison of simultaneous temperature observations by the GTS1 radiosonde and the Vaisala RS80 radiosonde suggests that these two radiosondes provide highly reproducible temperature measurements in the troposphere, but produce opposite biases for daytime and nighttime measurements in the stratosphere.In the stratosphere, the GTS1 shows a warm bias (＜0.5 K) in the daytime and a relatively large cool bias (-0.2 K to -1.6 K) at nighttime.     

MSB780(X)型数字气压计示值误差不确定度评定

MSB780(X)型数字气压计是民航机场气象台站常见的一种高精度气压传感器,其在保证客机安全着陆过程中的作用十分关键.对其进行示值误差的测量不确定度分析与评定有着极其重要的意义.依据JJG 1084—2013《数字式气压计检定规程》,对MSB780(X)型数字气压计进行示值误差的重复性测量.通过建立数学模型,对示值误差...