微型芦荟保健美容酒的研制

本文主要论述了以芦荟和泰米为主要原料利用微型啤酒生产设备生产保健美容米酒的生产过程和工艺参数。从而很好地解决了微型啤酒生产线利用率低的问题，并取得了很好的经济效益。     

南极磷虾油对小鼠肠黏膜屏障的保护作用及机制

为探究南极磷虾油对脂多糖所致肠黏膜屏障损伤的预防作用及其机制,选取32只雄性小鼠随机分为4组:正常对照组、模型组(脂多糖组)、南极磷虾油干预组和鱼油干预组.前两组灌胃橄榄油,后两组分别灌胃400 mg/kg(以体重计)以橄榄油为溶剂稀释的南极磷虾油或鱼油,每日1次.连续干预4周后,正常组小鼠经腹腔注射生理盐水,其余3组注射10 mg/kg(以体重计)脂多糖,6h后处死小鼠.苏木精-伊红染色后,观察小鼠小肠组织病理学变化;测定小鼠血清和小肠中二胺氧化酶活性,蛋白免疫印迹法分析紧密连接蛋白和诱导型一氧化氮合酶蛋白表达;检测髓过氧化物酶和一氧化氮含量,实时荧光定量PCR法检测促炎细胞因子mRNA表达;蛋白免疫印迹法分析TLR4/NF-κB信号通路相关蛋白表达水平.实验结果显示,南极磷虾油预防性干预能显著抑制脂多糖所致小鼠小肠绒毛长度与隐窝深度比值的降低;下调血清二胺氧化酶水平,提高小肠二胺氧化酶活性,增加肠道紧密连接蛋白Claudin-1、Occludin和ZO-1蛋白表达水平;抑制髓过氧化物酶活性、诱导性一氧化氮合酶蛋白表达和一氧化氮含量升高,降低TNF-α、IL-1β和IL-6的mRNA表达以及TLR4/NF-κB信号通路相关蛋白表达水平.研究结果表明,南极磷虾油可通过抑制TLR4/NF-κB信号通路预防脂多糖所致肠黏膜屏障损伤,且其效果优于传统鱼油.     

微米级薄膜的动态力学性能测试

利用动态热机械分析仪对微米级薄膜的动态力学性能进行了拉伸模式和剪切模式测试,得到了玻璃化转变温度、损耗因子、储能模量、损耗模量等参数.实验结果表明,DMA的剪切模式是一种理想、可靠的测试模式,可以提供更多的信息.     

Aberrant Sphingolipid Metabolism in the Human Fallopian Tube with Ectopic Pregnancy

Sphingosine 1-phosphate (S1P), a product of sphingomyelin metabolism, is generated via phosphorylation of sphingosine by sphingosine kinases (SphK). It acts via a family of G protein-coupled receptors or as an intracellular second messenger for agonists acting through the S1P receptors (S1P1–5). In our study, the expression of SphK1 and S1P1 was identified by immunohistochemistry and immunoblot. The concentration of S1P was measured using ELISA. The spontaneous contraction of isolated fallopian tube strips was determined by tension recording. Our results showed that SphK1 and S1P1 were localized in the fallopian tube epithelial cells. In addition, smooth muscle cells also contained S1P1. Compared with the intrauterine pregnancy group, SPHK1 and S1P1 were overexpressed in ectopic pregnancy. However, the S1P concentration within the human oviduct from ectopic pregnancy subjects was largely reduced than that from normal pregnancy subject. The results from tension recording indicated that exogenous and intracellularly generated S1P can regulate the spontaneous contraction of oviduct isolated from rats and human. In conclusion, the sphingolipid metabolism signal pathway functionally existed in the human fallopian tube. Aberrant sphingolipid metabolism in the human fallopian tube may be involved in ectopic pregnancy.     

盐渍海带加工过程中质量安全问题的研究

盐渍海带加工是沿海一带非常重要的产业之一。本文针对目前盐渍海带加工业的现状及存在问题,提出了切实可行的质量安全控制措施,具有较强的实践指导意义。     

Garlic Oil Suppressed the Hematological Disorders Induced by Chemotherapy and Radiotherapy in Tumor‐Bearing Mice

Although the anticancer effects of garlic and its products have been demonstrated by a variety of studies; however, few studies were conducted to investigate the effects of garlic on the adverse effects of chemo/radiotherapy. In order to clarify the above question and make a more comprehensive understanding of the anticancer effects of garlic, tumor xenograft mice model was established by subcutaneous injection of H22 tumor cells, and was used for the investigation of effects of garlic oil (GO) on the chemo/radiotherapy. In the chemotherapy test, tumor‐bearing mice were treated with cyclophosphamide (CTX) or CTX plus GO (25 or 50 mg/kg bw) for 14 d, while the mice received a single 5 Gy total body radiation or radiation plus GO (25 or 50 mg/kg bw) in radiotherapy test. The results showed that GO did not increase the tumor inhibitory rate of CTX/radiation, which indicated that GO could not enhance the chemo/radiosensitivity of cancer cells. However, the decrease of the peripheral total white blood cells (WBCs) count induced by CTX/radiation was significantly suppressed by GO cotreatment. Furthermore, GO cotreatment significantly inhibited the decrease of the DNA contents and the micronuclei ratio of the bone marrow. Lastly, the reduction of the endogenous spleen colonies induced by CTX/radiation was significantly suppressed by GO cotreatment. These findings support the idea that GO consumption may benefit for the cancer patients receiving chemotherapy or radiotherapy.     

青岛崂山百合科药用植物的资源多样性调查

目的 调查青岛崂山百合科药用植物资源的多样性,为崂山百合科植物资源保护及合理利用提供依据.方法 采用野外考察、标本采集与鉴定、资料收集等方法,在崂山进行为期2年的实地调查研究.结果 青岛崂山有百合科植物26属63种(含3变种),分别占山东省百合科植物资源总属数87％,总种数78％;其中药用植物62种,占比达98％.崂山百合科药用植物药用部位以根茎最多,其次为鳞茎,也有以花及全草入药;药用功能主要有解表、泻下、补虚;药用成分包括多糖、生物碱、甾体皂苷、黄酮等.结论 崂山百合科药用植物资源种类丰富,应在保护的前提下进行合理的开发利用.     

顶空气相色谱法测定左乙拉西坦中有机溶剂残留量

目的：建立顶空气相色谱法测定左乙拉西坦中有机溶剂残留量。方法：采用AgilentDB-624毛细管柱（30mX0．53mm,3．07m）;氮气为载气;进样口温度为220℃;检测器温度为250℃;毛细管柱温度为40℃。顶空进样,平衡温度为85℃,平衡时间为20min;以DMF为溶剂。结果：在本文色谱条件下,各残留溶剂均能得到良好的分离度,检测浓度在所考察的范围内与峰面积具有良好的线性,r=0．9990～0．9999;平均回收率为97．1％-102．5％,精密度试验及准确度试验的R．SD均小于3．5％。结论：本试验建立的色谱方法简便、准确,灵敏度高,适合左乙拉西坦有机溶剂残留量的检测。     

HPLC法测定甲磺酸伊马替尼片的有关物质

目的：建立高效液相色谱法测定甲磺酸伊马替尼片有关物质的方法。方法：色谱柱为KromasilCl8（250mm×4．6mm,5肛m）,以含0．04％辛烷磺酸钠的20mmol／L磷酸二氢钠溶液（磷酸调至PH2．5）-甲醇为流动相,梯度洗脱,流速为1ml．min-1,检测波长为268nm,柱温为30℃。结果：在本方法中,甲磺酸伊马替尼的线性关系良好,r为0．9999;专属性强,甲磺酸伊马替尼与各中间体及各降解产物之间都能完全分离;精密度良好,RSD为1％。结论：该方法准确、简便、灵敏,可用于甲磺酸伊马替尼片有关物质测定。     

利用行波电压分布特征的柔性直流输电线路单端故障定位

提出了一种基于分布参数模型的柔性直流输电线路单端故障定位原理。由于柔性直流输电线路两端并联有大电容,直流线路发生单极接地故障后,1模故障分量网络中的电压行波具有以下2个特点:在两端直流母线处全反射,故障点处主要为折射;反射改变极性,折射不改变极性。据此可以在1模故障分量网络中计算本端第1次电压反射波及其前行路径上的电压分布,找出该电压分布的最强正跳变点,该点到对端的距离即为故障距离。该方法的故障定位精度高,理论上不受过渡电阻的影响,无需人工识别行波波头,易于实现故障定位的自动化,仿真结果表明该方法具有全线的适用性。